HAMAP rule MF_00174
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_00174 |
| Accession | MF_00174 |
| Dates | 1-JUN-2001 (Created)
5-NOV-2024 (Last updated, Version 28) |
| Name | EF_P_modif_A |
| Scope(s) |
Bacteria Gammaproteobacteria |
| Template(s) | P0A8N7 (EPMA_ECOLI); Q9ZJ12 (EPMA_SALTY); [ Recover all ] |
| Triggered by |
HAMAP; MF_00174 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | EPMA |
| Protein name | RecName: Full=Elongation factor P--(R)-beta-lysine ligase; Short=EF-P--(R)-beta-lysine ligase; EC=6.3.1.-; AltName: Full=EF-P-lysine lysyltransferase; AltName: Full=EF-P post-translational modification enzyme A; |
| Gene name | Name=epmA; |
Comments
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| case <OC:Escherichia> or <OC:Shigella> or <OC:Salmonella> | |
| FUNCTION | With EpmB is involved in the beta-lysylation step of the post-translational modification of translation elongation factor P (EF- P) on 'Lys-34'. Catalyzes the ATP-dependent activation of (R)-beta- lysine produced by EpmB, forming a lysyl-adenylate, from which the beta-lysyl moiety is then transferred to the epsilon-amino group of EF- P 'Lys-34'. |
| else | |
| FUNCTION | With EpmB is involved in the beta-lysylation step of the post-translational modification of translation elongation factor P (EF- P). Catalyzes the ATP-dependent activation of (R)-beta-lysine produced by EpmB, forming a lysyl-adenylate, from which the beta-lysyl moiety is then transferred to the epsilon-amino group of a conserved specific lysine residue in EF-P. |
| end case | |
| SUBUNIT | Homodimer. |
| SIMILARITY | Belongs to the class-II aminoacyl-tRNA synthetase family. EpmA subfamily. |
Keywords
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Gene Ontology
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| GO:0005524; Molecular function:ATP binding |
| GO:0016880; Molecular function:acid-ammonia (or amide) ligase activity |
Cross-references
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| Pfam | PF00152; tRNA-synt_2; 1; |
| PRINTS | PR00982; TRNASYNTHLYS; 1; |
| NCBIfam | TIGR00462; GenX; 1; |
| PROSITE | PS50862; AA_TRNA_LIGASE_II; 1; |
Features
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| From: EPMA_ECOLI (P0A8N7) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 100 | 102 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
R-x-[EQKN] | ||||||||
| BINDING | 244 | 245 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
E-L | ||||||||
| BINDING | 76 | 78 | /ligand="substrate" | S-x-E | ||||||||
| BINDING | 109 | 109 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
N | ||||||||
| BINDING | 118 | 118 | /ligand="substrate" | Y | ||||||||
| BINDING | 251 | 251 | /ligand="substrate" | E | ||||||||
| BINDING | 300 | 300 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" |
G | ||||||||
Additional information
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| Size range | 293-327 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |