[1]	NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA AND BETA). TISSUE=Brain; Zhu X., Boulay G., Jiang M., Birnbaumer L.; "Trp4 is involved in capacitative calcium entry in murine cells."; Submitted (AUG-1997) to the EMBL/GenBank/DDBJ databases.
[2]	NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA AND BETA). Qian F., Philipson L.H.; Submitted (MAR-1996) to the EMBL/GenBank/DDBJ databases.
[3]	NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA AND BETA). TISSUE=Brain; PubMed=9512398 [NCBI, ExPASy, EBI, Israel, Japan] Mori Y., Takada N., Okada T., Wakamori M., Imoto K., Wanifuchi H., Oka H., Oba A., Ikenaka K., Kurosaki T.; "Differential distribution of TRP Ca2+ channel isoforms in mouse brain."; NeuroReport 9:507-515(1998).
[4]	NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS ALPHA AND BETA). DOI=10.1074/jbc.M006635200; PubMed=10980202 [NCBI, ExPASy, EBI, Israel, Japan] Tang Y., Tang J., Chen Z., Trost C., Flockerzi V., Li M., Ramesh V., Zhu M.X.; "Association of mammalian trp4 and phospholipase C isozymes with a PDZ domain-containing protein, NHERF."; J. Biol. Chem. 275:37559-37564(2000).
[5]	NUCLEOTIDE SEQUENCE [MRNA] OF 505-642. TISSUE=Brain; PubMed=7575478 [NCBI, ExPASy, EBI, Israel, Japan] Petersen C.C.H., Berridge M.J., Borgese M.F., Bennett D.L.; "Putative capacitative calcium entry channels: expression of Drosophila trp and evidence for the existence of vertebrate homologues."; Biochem. J. 311:41-44(1995).
[6]	FUNCTION. DOI=10.1038/35055019; PubMed=11175743 [NCBI, ExPASy, EBI, Israel, Japan] Freichel M., Suh S.H., Pfeifer A., Schweig U., Trost C., Weissgerber P., Biel M., Philipp S., Freise D., Droogmans G., Hofmann F., Flockerzi V., Nilius B.; "Lack of an endothelial store-operated Ca2+ current impairs agonist-dependent vasorelaxation in TRP4-/- mice."; Nat. Cell Biol. 3:121-127(2001).

Comments

FUNCTION: Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Has also been shown to be calcium-selective (By similarity). May also be activated by intracellular calcium store depletion. TRPC4 deficient mice lack a store-operated calcium entry in endothelial cells.
SUBUNIT: Isoform alpha but not isoform beta associates with inositol-1,4,5-triphosphate receptor (ITPR) (By similarity). Interacts with NHERF. Interacts with MX1 and RNF24 (By similarity).
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein (Probable).
ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative splicing.

Name Alpha

Isoform ID Q9QUQ5-1

This is the isoform sequence displayed in this entry.

Name Beta

Isoform ID Q9QUQ5-2

Features which should be applied to build the isoform sequence: VSP_006570.
TISSUE SPECIFICITY: Abundantly expressed in brain (hippocampal CA1 pyramidal neurons, dentate gyrus granule cells, and cerebral cortical neurons, and in the septal nuclei and the mitral layer of olfactory bulb). Lower levels are detected in other tissues.
SIMILARITY: Belongs to the transient receptor family. STrpC subfamily.
SIMILARITY: Contains 2 ANK repeats.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AF011543; AAD10167.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U50922; AAC05179.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF190646; AAF01469.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
U50921; AAC05178.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF019663; AAD10168.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
X90697; CAA62230.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

IPI

IPI00132045; -.
IPI00229745; -.

S59128; S59128.

NP_058680.1; -.

3D structure databases

ModBase

Q9QUQ5.

PTM databases

PhosphoSite

Q9QUQ5; -.

Organism-specific databases

MGI

MGI:109525; Trpc4.

Gene expression databases

ArrayExpress

Q9QUQ5; -.

Bgee

Q9QUQ5; -.

GermOnline

ENSMUSG00000027748; Mus musculus.

Ontologies

GO:0005901;	Cellular component: caveola (inferred from direct assay from MGI).
GO:0016021;	Cellular component: integral to membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0043234;	Cellular component: protein complex (inferred from physical interaction from MGI).
GO:0005509;	Molecular function: calcium ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0005515;	Molecular function: protein binding (inferred from physical interaction from MGI).
GO:0015279;	Molecular function: store-operated calcium channel activity (inferred from mutant phenotype from MGI).
GO:0006816;	Biological process: calcium ion transport (inferred from electronic annotation from UniProtKB-KW).
GO:0014051;	Biological process: gamma-aminobutyric acid secretion (inferred from mutant phenotype from MGI).
QuickGo view.

Family and domain databases

InterPro

IPR002110; ANK.
IPR005821; Ion_trans.
IPR002153; Trans_rcpt.
IPR013555; TRP_2.
IPR004729; TRP_channel.
IPR005460; TRP_channel4.
Graphical view of domain structure.

Gene3D

G3DSA:1.25.40.20; ANK; 1.

Pfam

PF00023; Ank; 2.
PF00520; Ion_trans; 1.
PF08344; TRP_2; 1.
Pfam graphical view of domain structure.

PRINTS

PR01097; TRNSRECEPTRP.
PR01645; TRPCHANNEL4.

SMART

SM00248; ANK; 2.
SMART graphical view of domain structure.

TIGRFAMs

TIGR00870; trp; 1.

PROSITE

PS50297; ANK_REP_REGION; 1.
PS50088; ANK_REPEAT; 1.
PROSITE graphical view of domain structure (profiles).

Genome annotation databases

Ensembl

ENSMUSG00000027748; Mus musculus. [Contig view]

GeneID

22066; -.

KEGG

mmu:22066; -.

Phylogenomic databases

HOGENOM

Q9QUQ5; -.

HOVERGEN

Q9QUQ5; -.

OMA

Q9QUQ5; DGGFQDY.

Other

301884; -.

Trpc4; Mus musculus.

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Alternative splicing; ANK repeat; Calcium; Calcium channel; Calcium transport; Ion transport; Ionic channel; Membrane; Phosphoprotein; Repeat; Transmembrane; Transport.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 974`	`974`	`Short transient receptor potential channel 4.`	`PRO_0000215315`
`TOPO_DOM`	`1 329`	`329`	`Cytoplasmic (Potential).`
`TRANSMEM`	`330 350`	`21`	`Potential.`
`TOPO_DOM`	`351 362`	`12`	`Extracellular (Potential).`
`TRANSMEM`	`363 383`	`21`	`Potential.`
`TOPO_DOM`	`384 436`	`53`	`Cytoplasmic (Potential).`
`TRANSMEM`	`437 457`	`21`	`Potential.`
`TOPO_DOM`	`458 469`	`12`	`Extracellular (Potential).`
`TRANSMEM`	`470 490`	`21`	`Potential.`
`TOPO_DOM`	`491 511`	`21`	`Cytoplasmic (Potential).`
`TRANSMEM`	`512 532`	`21`	`Potential.`
`TOPO_DOM`	`533 599`	`67`	`Extracellular (Potential).`
`TRANSMEM`	`600 620`	`21`	`Potential.`
`TOPO_DOM`	`621 974`	`354`	`Cytoplasmic (Potential).`
`REPEAT`	`69 98`	`30`	`ANK 1.`
`REPEAT`	`141 170`	`30`	`ANK 2.`
`REGION`	`615 974`	`360`	`Binds to ITPR1, ITPR2 and ITPR3 (By similarity).`
`REGION`	`972 974`	`3`	`Essential for binding to NHERF PDZ domain.`
`MOD_RES`	`193 193`		`Phosphoserine (By similarity).`
`MOD_RES`	`195 195`		`Phosphoserine (By similarity).`
`VAR_SEQ`	`781 864`		`Missing (in isoform Beta).`	`VSP_006570`
`CONFLICT`	`780 780`		`E -> K (in Ref. 3; AAF01469).`
`CONFLICT`	`890 890`		`R -> L (in Ref. 3; AAF01469).`

Sequence information

Length: 974 AA [This is the length of the unprocessed precursor]

Molecular weight: 111575 Da [This is the MW of the unprocessed precursor]

CRC64: 2D0BB2F235F5F8D1 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAQFYYKRNV NAPYRDRIPL RIVRAESELS PSEKAYLNAV EKGDYASVKK SLEEAEIYFK 

        70         80         90        100        110        120 
ININCIDPLG RTALLIAIEN ENLELIELLL SFNVYVGDAL LHAIRKEVVG AVELLLNHKK 

       130        140        150        160        170        180 
PSGEKQVPPI LLDKQFSEFT PDITPIILAA HTNNYEIIKL LVQKGVSVPR PHEVRCNCVE 

       190        200        210        220        230        240 
CVSSSDVDSL RHSRSRLNIY KALASPSLIA LSSEDPFLTA FQLSWELQEL SKVENEFKSE 

       250        260        270        280        290        300 
YEELSRQCKQ FAKDLLDQTR SSRELEIILN YRDDNSLIEE QSGNDLARLK LAIKYRQKEF 

       310        320        330        340        350        360 
VAQPNCQQLL ASRWYDEFPG WRRRHWAVKM VTCFIIGLLF PVFSVCYLIA PKSPLGLFIR 

       370        380        390        400        410        420 
KPFIKFICHT ASYLTFLFLL LLASQHIDRS DLNRQGPPPT IVEWMILPWV LGFIWGEIKQ 

       430        440        450        460        470        480 
MWDGGLQDYI HDWWNLMDFV MNSLYLATIS LKIVAFVKYS ALNPRESWDM WHPTLVAEAL 

       490        500        510        520        530        540 
FAIANIFSSL RLISLFTANS HLGPLQISLG RMLLDILKFL FIYCLVLLAF ANGLNQLYFY 

       550        560        570        580        590        600 
YEETKGLSCK GIRCEKQNNA FSTLFETLQS LFWSIFGLIN LYVTNVKAQH EFTEFVGATM 

       610        620        630        640        650        660 
FGTYNVISLV VLLNMLIAMM NNSYQLIADH ADIEWKFART KLWMSYFEEG GTLPTPFNVI 

       670        680        690        700        710        720 
PSPKSLWYLV KWIWTHLCKK KMRRKPESFG TIGRRAADNL RRHHQYQEVM RNLVKRYVAA 

       730        740        750        760        770        780 
MIREAKTEEG LTEENVKELK QDISSFRFEV LGLLRGSKLS TIQSANAASS ADSDEKSQSE 

       790        800        810        820        830        840 
GNGKDKRKNL SLFDLTTLIH PRSAAIASER HNLSNGSALV VQEPPREKQR KVNFVADIKN 

       850        860        870        880        890        900 
FGLFHRRSKQ NAAEQNANQI FSVSEEITRQ QAAGALERNI ELESKGLASR GDRSIPGLNE 

       910        920        930        940        950        960 
QCVLVDHRER NTDTLGLQVG KRVCSTFKSE KVVVEDTVPI IPKEKHAHEE DSSIDYDLSP 

       970 
TDTAAHEDYV TTRL

Q9QUQ5 in FASTA format

View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools