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UniProtKB/Swiss-Prot entry Q9JM54


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name APR_MOUSE
Primary accession number Q9JM54
Secondary accession numbers None
Integrated into Swiss-Prot on May 20, 2008
Sequence was last modified on October 1, 2000 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 53)
Name and origin of the protein
Protein name Phorbol-12-myristate-13-acetate-induced protein 1
Synonym Noxa
Gene name
Name: Pmaip1
Synonyms: Noxa
From
Mus musculus (Mouse) [TaxID: 10090] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA], INTERACTION WITH MCL1, SUBCELLULAR LOCATION, FUNCTION, AND MUTAGENESIS OF LEU-27 AND LEU-78.
DOI=10.1126/science.288.5468.1053; PubMed=10807576 [NCBI, ExPASy, EBI, Israel, Japan]
Oda E., Ohki R., Murasawa H., Nemoto J., Shibue T., Yamashita T., Tokino T., Taniguchi T., Tanaka N.;
"Noxa, a BH3-only member of the Bcl-2 family and candidate mediator of p53-induced apoptosis.";
Science 288:1053-1058(2000).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=C57BL/6J, and NOD;
TISSUE=Brain cortex, Dendritic cell, Kidney, and Thymus;
DOI=10.1126/science.1112014; PubMed=16141072 [NCBI, ExPASy, EBI, Israel, Japan]
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J., Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
"The transcriptional landscape of the mammalian genome.";
Science 309:1559-1563(2005).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Limb;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[4]
FUNCTION, AND INTERACTION WITH MCL1.
DOI=10.1101/gad.1304105; PubMed=15901672 [NCBI, ExPASy, EBI, Israel, Japan]
Willis S.N., Chen L., Dewson G., Wei A., Naik E., Fletcher J.I., Adams J.M., Huang D.C.S.;
"Proapoptotic Bak is sequestered by Mcl-1 and Bcl-xL, but not Bcl-2, until displaced by BH3-only proteins.";
Genes Dev. 19:1294-1305(2005).
[5]
FUNCTION, AND INTERACTION WITH MCL1.
DOI=10.1016/j.molcel.2004.12.030; PubMed=15694340 [NCBI, ExPASy, EBI, Israel, Japan]
Chen L., Willis S.N., Wei A., Smith B.J., Fletcher J.I., Hinds M.G., Colman P.M., Day C.L., Adams J.M., Huang D.C.S.;
"Differential targeting of prosurvival Bcl-2 proteins by their BH3-only ligands allows complementary apoptotic function.";
Mol. Cell 17:393-403(2005).
[6]
FUNCTION, INDUCTION, AND TISSUE SPECIFICITY.
DOI=10.1523/JNEUROSCI.0196-06.2006; PubMed=16822983 [NCBI, ExPASy, EBI, Israel, Japan]
Akhtar R.S., Geng Y., Klocke B.J., Latham C.B., Villunger A., Michalak E.M., Strasser A., Carroll S.L., Roth K.A.;
"BH3-only proapoptotic Bcl-2 family members Noxa and Puma mediate neural precursor cell death.";
J. Neurosci. 26:7257-7264(2006).
[7]
X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 68-93 IN COMPLEX WITH MCL1, STRUCTURE BY NMR OF 68-94 IN COMPLEX WITH MCL1, FUNCTION, MASS SPECTROMETRY, AND INTERACTION WITH MCL1.
DOI=10.1073/pnas.0701297104; PubMed=17389404 [NCBI, ExPASy, EBI, Israel, Japan]
Czabotar P.E., Lee E.F., van Delft M.F., Day C.L., Smith B.J., Huang D.C.S., Fairlie W.D., Hinds M.G., Colman P.M.;
"Structural insights into the degradation of Mcl-1 induced by BH3 domains.";
Proc. Natl. Acad. Sci. U.S.A. 104:6217-6222(2007).
Comments
  • FUNCTION: Promotes activation of caspases and apoptosis. Promotes mitochondrial membrane changes and efflux of apoptogenic proteins from the mitochondria. Contributes to p53-dependent apoptosis after radiation exposure. Promotes proteasomal degradation of MCL1. Competes with BIM/BCL2L11 for binding to MCL1 and can displace BIM/BCL2L11 from its binding site on MCL1 (By similarity). Competes with BAK1 for binding to MCL1 and can displace BAK1 from its binding site on MCL1.
  • SUBUNIT: Interacts with MCL1 and BAX (By similarity).
  • INTERACTION:
    Q07820:MCL1 (xeno); NbExp=1; IntAct=EBI-709183, EBI-1003422;
    P97287:Mcl1; NbExp=1; IntAct=EBI-709183, EBI-707292;
  • SUBCELLULAR LOCATION: Mitochondrion.
  • TISSUE SPECIFICITY: Detected in thymocytes after irradiation with X-rays. Not detectable in untreated thymocytes (at protein level). Detected in embryonic neural precursor cells of the telencephalon Constitutively expressed at low levels in adult brain, testis, thymus, spleen, lung and kidney.
  • INDUCTION: Up-regulated after exposure to ionizing radiation and other genotoxic agents. Up-regulation is mediated by p53.
  • DOMAIN: The BH3 motif is essential for pro-apoptotic activity.
  • SIMILARITY: Belongs to the PMAIP1 family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AB041230; BAA95781.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK043856; BAC31682.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK088556; BAC40421.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK143990; BAE25650.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK169914; BAE41454.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC050821; AAH50821.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_067426.1; -.
UniGene Mm.271878
3D structure databases
PDB
2JM6; NMR; -; A=68-94.[ExPASy / RCSB / EBI]
2NLA; X-ray; 2.80 A; B=68-93.[ExPASy / RCSB / EBI]
Detailed list of linked structures.
PDBsum 2JM6; -.
2NLA; -.
ModBase Q9JM54.
Protein-protein interaction databases
IntAct Q9JM54; -.
Organism-specific databases
MGI MGI:1930146; Pmaip1.
Gene expression databases
ArrayExpress Q9JM54; -.
Ontologies
GO
GO:0005741; Cellular component: mitochondrial outer membrane (inferred from experiment from Reactome).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
GO:0008629; Biological process: induction of apoptosis by intracellular signals (inferred from mutant phenotype from MGI).
GO:0048147; Biological process: negative regulation of fibroblast proliferation (inferred from mutant phenotype from MGI).
GO:0043525; Biological process: positive regulation of neuron apoptosis (inferred from mutant phenotype from MGI).
GO:0001844; Biological process: protein insertion into mitochondrial membrane during induction of apoptosis (inferred from mutant phenotype from MGI).
GO:0001836; Biological process: release of cytochrome c from mitochondria (inferred from mutant phenotype from MGI).
GO:0006974; Biological process: response to DNA damage stimulus (inferred from mutant phenotype from MGI).
GO:0009411; Biological process: response to UV (inferred from mutant phenotype from MGI).
GO:0010165; Biological process: response to X-ray (inferred from mutant phenotype from MGI).
QuickGo view.
Family and domain databases
PROSITE PS01259; BH3; FALSE_NEG.
BLOCKS Q9JM54.
Genome annotation databases
Ensembl ENSMUSG00000024521; Mus musculus. [Contig view]
GeneID 58801; -.
KEGG mmu:58801; -.
Phylogenomic databases
HOGENOM Q9JM54; -.
HOVERGEN Q9JM54; -.
Other
SOURCE Pmaip1; Mus musculus.
ProtoNet Q9JM54.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Apoptosis; Mitochondrion; Repeat.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom  To Length Description FTId
CHAIN   1   103  103     Phorbol-12-myristate-13-acetate-induced protein 1. PRO_0000333230
REGION   90    99  10     Required for mitochondrial location (By similarity). 
MOTIF   27    35  9     BH3 1. 
MOTIF   78    86  9     BH3 2. 
MUTAGEN   27    27        L->A: Loss of pro-apoptotic activity and of targeting to mitochondria; when associated with A-78. 
MUTAGEN   78    78        L->A: Loss of pro-apoptotic activity and of targeting to mitochondria; when associated with A-27. 
Sequence information
Length: 103 AA [This is the length of the unprocessed precursor] Molecular weight: 11566 Da [This is the MW of the unprocessed precursor] CRC64: 9B9A5B04D5535E30 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MPGRKARRNA PVNPTRAELP PEFAAQLRKI GDKVYCTWSA PDITVVLAQM PGKSQKSRMR 

        70         80         90        100 
SPSPTRVPAD LKDECAQLRR IGDKVNLRQK LLNLISKLFN LVT 

Q9JM54 in FASTA format

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