ID   MLP3B_HUMAN             Reviewed;         125 AA.
AC   Q9GZQ8; Q6NW02;
DT   05-DEC-2001, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   22-JUL-2008, entry version 72.
DE   RecName: Full=Microtubule-associated proteins 1A/1B light chain 3B;
DE   AltName: Full=Microtubule-associated protein 1 light chain 3 beta;
DE   AltName: Full=MAP1A/1B light chain 3 B;
DE   AltName: Full=MAP1A/MAP1B LC3 B;
DE   AltName: Full=MAP1 light chain 3-like protein 2;
DE   AltName: Full=Autophagy-related protein LC3 B;
DE   AltName: Full=Autophagy-related ubiquitin-like modifier LC3 B;
DE   Flags: Precursor;
GN   Name=MAP1LC3B; Synonyms=MAP1ALC3;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Lung carcinoma;
RX   PubMed=12932674; DOI=10.1016/S0959-8049(03)00419-2;
RA   Difilippantonio S., Chen Y., Pietas A., Schluens K.,
RA   Pacyna-Gengelbach M., Deutschmann N., Padilla-Nash H.M., Ried T.,
RA   Petersen I.;
RT   "Gene expression profiles in human non-small and small-cell lung
RT   cancers.";
RL   Eur. J. Cancer 39:1936-1947(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Pheochromocytoma;
RX   PubMed=14713272; DOI=10.1677/erc.0.0100621;
RA   Yang Y.-S., Song H.-D., Peng Y.-D., Huang Q.-H., Li R.-Y., Zhu Z.-D.,
RA   Hu R.-M., Han Z.-G., Chen J.-L.;
RT   "The gene expression profiling of sporadic pheochromocytoma and novel
RT   full-length cDNAs cloning.";
RL   Endocr. Relat. Cancer 10:621-627(2003).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, AND TISSUE
RP   SPECIFICITY.
RX   MEDLINE=22765575; PubMed=12740394; DOI=10.1074/jbc.M303800200;
RA   He H., Dang Y., Dai F., Guo Z., Wu J., She X., Pei Y., Chen Y.,
RA   Ling W., Wu C., Zhao S., Liu J.O., Yu L.;
RT   "Post-translational modifications of three members of the human
RT   MAP1LC3 family and detection of a novel type of modification for
RT   MAP1LC3B.";
RL   J. Biol. Chem. 278:29278-29287(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Hepatoma;
RX   PubMed=14702039; DOI=10.1038/ng1285;
RA   Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA   Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA   Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA   Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA   Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A.,
RA   Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M.,
RA   Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y.,
RA   Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M.,
RA   Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K.,
RA   Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S.,
RA   Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J.,
RA   Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y.,
RA   Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N.,
RA   Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S.,
RA   Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA   Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA   Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA   Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA   Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y.,
RA   Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T.,
RA   Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y.,
RA   Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S.,
RA   Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T.,
RA   Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M.,
RA   Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T.,
RA   Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K.,
RA   Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R.,
RA   Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
RT   "Complete sequencing and characterization of 21,243 full-length human
RT   cDNAs.";
RL   Nat. Genet. 36:40-45(2004).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain, Pancreas, and Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [6]
RP   LIPIDATION, AND CLEAVAGE BY APG4B.
RX   PubMed=15187094; DOI=10.1074/jbc.M401461200;
RA   Tanida I., Sou Y.-S., Ezaki J., Minematsu-Ikeguchi N., Ueno T.,
RA   Kominami E.;
RT   "HsAtg4B/HsApg4B/autophagin-1 cleaves the carboxyl termini of three
RT   human Atg8 homologues and delipidates microtubule-associated protein
RT   light chain 3- and GABAA receptor-associated protein-phospholipid
RT   conjugates.";
RL   J. Biol. Chem. 279:36268-36276(2004).
RN   [7]
RP   CLEAVAGE, AND MUTAGENESIS OF GLY-120 AND LYS-122.
RX   PubMed=15355958; DOI=10.1074/jbc.M407016200;
RA   Tanida I., Ueno T., Kominami E.;
RT   "Human light chain 3/MAP1LC3B is cleaved at its carboxyl-terminal
RT   Met121 to expose Gly120 for lipidation and targeting to autophagosomal
RT   membranes.";
RL   J. Biol. Chem. 279:47704-47710(2004).
RN   [8]
RP   REVIEW.
RX   PubMed=15325588; DOI=10.1016/j.biocel.2004.05.009;
RA   Tanida I., Ueno T., Kominami E.;
RT   "LC3 conjugation system in mammalian autophagy.";
RL   Int. J. Biochem. Cell Biol. 36:2503-2518(2004).
CC   -!- FUNCTION: Probably involved in formation of autophagosomal
CC       vacuoles (autophagosomes).
CC   -!- SUBUNIT: 3 different light chains, LC1, LC2 and LC3, can associate
CC       with MAP1A and MAP1B proteins (By similarity).
CC   -!- INTERACTION:
CC       Q13501:SQSTM1; NbExp=1; IntAct=EBI-373144, EBI-307104;
CC       Q64337:Sqstm1 (xeno); NbExp=2; IntAct=EBI-373144, EBI-645025;
CC       Q9HAU5:UPF2; NbExp=1; IntAct=EBI-373144, EBI-372073;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm. Intracytoplasmic membrane; Lipid-
CC       anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor.
CC       Note=LC3-II binds to the autophagic membranes.
CC   -!- TISSUE SPECIFICITY: Most abundant in heart, brain, skeletal muscle
CC       and testis. Little expression observed in liver.
CC   -!- PTM: The precursor molecule is cleaved by APG4B/ATG4B to form LC3-
CC       I. This is activated by APG7L/ATG7, transferred to ATG3 and
CC       conjugated to phospholipid to form LC3-II.
CC   -!- SIMILARITY: Belongs to the MAP1 LC3 family.
CC   -!- CAUTION: PubMed:12740394 has shown that the protein is cleaved at
CC       Lys-122 but PubMed:15355958 has shown that the cleavage site is at
CC       Gly-120 as in other mammalian orthologs.
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DR   EMBL; AF303888; AAG23182.1; -; mRNA.
DR   EMBL; AF183417; AAG09686.1; -; mRNA.
DR   EMBL; AF087871; AAM10499.1; -; mRNA.
DR   EMBL; AK025556; BAB15169.1; -; mRNA.
DR   EMBL; BC018634; AAH18634.1; -; mRNA.
DR   EMBL; BC041874; AAH41874.1; -; mRNA.
DR   EMBL; BC067797; AAH67797.1; -; mRNA.
DR   RefSeq; NP_073729.1; -.
DR   UniGene; Hs.356061; -.
DR   PDB; 1V49; NMR; -; A=1-120.
DR   PDBsum; 1V49; -.
DR   IntAct; Q9GZQ8; -.
DR   Ensembl; ENSG00000140941; Homo sapiens.
DR   GeneID; 81631; -.
DR   KEGG; hsa:81631; -.
DR   H-InvDB; HIX0013321; -.
DR   H-InvDB; HIX0035198; -.
DR   H-InvDB; HIX0059104; -.
DR   HGNC; HGNC:13352; MAP1LC3B.
DR   MIM; 609604; gene.
DR   PharmGKB; PA134923100; -.
DR   HOGENOM; Q9GZQ8; -.
DR   HOVERGEN; Q9GZQ8; -.
DR   LinkHub; Q9GZQ8; -.
DR   ArrayExpress; Q9GZQ8; -.
DR   CleanEx; HS_MAP1LC3B; -.
DR   GermOnline; ENSG00000140941; Homo sapiens.
DR   GO; GO:0005776; C:autophagic vacuole; ISS:UniProtKB.
DR   GO; GO:0031090; C:organelle membrane; ISS:UniProtKB.
DR   GO; GO:0005515; F:protein binding; IPI:UniProtKB.
DR   InterPro; IPR004241; MAP1_LC3.
DR   PANTHER; PTHR10969; MAP1_LC3; 1.
DR   Pfam; PF02991; MAP1_LC3; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Autophagy; Cytoplasm; Cytoplasmic vesicle; Lipoprotein;
KW   Membrane; Microtubule; Ubl conjugation pathway.
FT   INIT_MET      1      1       Removed (By similarity).
FT   CHAIN         2    120       Microtubule-associated proteins 1A/1B
FT                                light chain 3B.
FT                                /FTId=PRO_0000017198.
FT   PROPEP      121    125       Removed in mature form.
FT                                /FTId=PRO_0000017199.
FT   LIPID       120    120       Phosphatidylethanolamine amidated glycine
FT                                (Probable).
FT   MUTAGEN     120    120       G->A: No processing of precursor.
FT   MUTAGEN     122    122       K->A: No effect on processing of
FT                                precursor.
FT   CONFLICT     61     61       S -> G (in Ref. 5; AAH67797).
FT   HELIX         7     10
FT   HELIX        13     26
FT   STRAND       30     37
FT   STRAND       50     55
FT   HELIX        60     70
FT   TURN         75     77
FT   STRAND       80     83
FT   HELIX        95    102
FT   STRAND      109    115
SQ   SEQUENCE   125 AA;  14688 MW;  BB141DEC7653E83F CRC64;
     MPSEKTFKQR RTFEQRVEDV RLIREQHPTK IPVIIERYKG EKQLPVLDKT KFLVPDHVNM
     SELIKIIRRR LQLNANQAFF LLVNGHSMVS VSTPISEVYE SEKDEDGFLY MVYASQETFG
     MKLSV
//