ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!
Search for

UniProtKB/Swiss-Prot entry Q9GZQ8


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

Note: most headings are clickable, even if they don't appear as links. They link to the user manual or other documents.
Entry information
Entry name MLP3B_HUMAN
Primary accession number Q9GZQ8
Secondary accession number Q6NW02
Integrated into Swiss-Prot on December 5, 2001
Sequence was last modified on January 23, 2007 (Sequence version 3)
Annotations were last modified on    July 22, 2008 (Entry version 72)
Name and origin of the protein
Protein name Microtubule-associated proteins 1A/1B light chain 3B [Precursor]
Synonyms Microtubule-associated protein 1 light chain 3 beta
MAP1A/1B light chain 3 B
MAP1A/MAP1B LC3 B
MAP1 light chain 3-like protein 2
Autophagy-related protein LC3 B
Autophagy-related ubiquitin-like modifier LC3 B
Gene name
Name: MAP1LC3B
Synonyms: MAP1ALC3
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Lung carcinoma;
DOI=10.1016/S0959-8049(03)00419-2; PubMed=12932674 [NCBI, ExPASy, EBI, Israel, Japan]
Difilippantonio S., Chen Y., Pietas A., Schluens K., Pacyna-Gengelbach M., Deutschmann N., Padilla-Nash H.M., Ried T., Petersen I.;
"Gene expression profiles in human non-small and small-cell lung cancers.";
Eur. J. Cancer 39:1936-1947(2003).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Pheochromocytoma;
DOI=10.1677/erc.0.0100621; PubMed=14713272 [NCBI, ExPASy, EBI, Israel, Japan]
Yang Y.-S., Song H.-D., Peng Y.-D., Huang Q.-H., Li R.-Y., Zhu Z.-D., Hu R.-M., Han Z.-G., Chen J.-L.;
"The gene expression profiling of sporadic pheochromocytoma and novel full-length cDNAs cloning.";
Endocr. Relat. Cancer 10:621-627(2003).
[3]
NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
DOI=10.1074/jbc.M303800200; PubMed=12740394 [NCBI, ExPASy, EBI, Israel, Japan]
He H., Dang Y., Dai F., Guo Z., Wu J., She X., Pei Y., Chen Y., Ling W., Wu C., Zhao S., Liu J.O., Yu L.;
"Post-translational modifications of three members of the human MAP1LC3 family and detection of a novel type of modification for MAP1LC3B.";
J. Biol. Chem. 278:29278-29287(2003).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Hepatoma;
DOI=10.1038/ng1285; PubMed=14702039 [NCBI, ExPASy, EBI, Israel, Japan]
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S., Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.;
"Complete sequencing and characterization of 21,243 full-length human cDNAs.";
Nat. Genet. 36:40-45(2004).
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Brain, Pancreas, and Testis;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[6]
LIPIDATION, AND CLEAVAGE BY APG4B.
DOI=10.1074/jbc.M401461200; PubMed=15187094 [NCBI, ExPASy, EBI, Israel, Japan]
Tanida I., Sou Y.-S., Ezaki J., Minematsu-Ikeguchi N., Ueno T., Kominami E.;
"HsAtg4B/HsApg4B/autophagin-1 cleaves the carboxyl termini of three human Atg8 homologues and delipidates microtubule-associated protein light chain 3- and GABAA receptor-associated protein-phospholipid conjugates.";
J. Biol. Chem. 279:36268-36276(2004).
[7]
CLEAVAGE, AND MUTAGENESIS OF GLY-120 AND LYS-122.
DOI=10.1074/jbc.M407016200; PubMed=15355958 [NCBI, ExPASy, EBI, Israel, Japan]
Tanida I., Ueno T., Kominami E.;
"Human light chain 3/MAP1LC3B is cleaved at its carboxyl-terminal Met121 to expose Gly120 for lipidation and targeting to autophagosomal membranes.";
J. Biol. Chem. 279:47704-47710(2004).
[8]
REVIEW.
DOI=10.1016/j.biocel.2004.05.009; PubMed=15325588 [NCBI, ExPASy, EBI, Israel, Japan]
Tanida I., Ueno T., Kominami E.;
"LC3 conjugation system in mammalian autophagy.";
Int. J. Biochem. Cell Biol. 36:2503-2518(2004).
Comments
  • FUNCTION: Probably involved in formation of autophagosomal vacuoles (autophagosomes).
  • SUBUNIT: 3 different light chains, LC1, LC2 and LC3, can associate with MAP1A and MAP1B proteins (By similarity).
  • INTERACTION:
    Q13501:SQSTM1; NbExp=1; IntAct=EBI-373144, EBI-307104;
    Q64337:Sqstm1 (xeno); NbExp=2; IntAct=EBI-373144, EBI-645025;
    Q9HAU5:UPF2; NbExp=1; IntAct=EBI-373144, EBI-372073;
  • SUBCELLULAR LOCATION: Cytoplasm. Intracytoplasmic membrane; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Note=LC3-II binds to the autophagic membranes.
  • TISSUE SPECIFICITY: Most abundant in heart, brain, skeletal muscle and testis. Little expression observed in liver.
  • PTM: The precursor molecule is cleaved by APG4B/ATG4B to form LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form LC3-II.
  • SIMILARITY: Belongs to the MAP1 LC3 family.
  • CAUTION: PubMed:12740394 has shown that the protein is cleaved at Lys-122 but PubMed:15355958 has shown that the cleavage site is at Gly-120 as in other mammalian orthologs.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AF303888; AAG23182.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF183417; AAG09686.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF087871; AAM10499.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK025556; BAB15169.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC018634; AAH18634.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC041874; AAH41874.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC067797; AAH67797.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_073729.1; -.
UniGene Hs.356061
3D structure databases
PDB
1V49; NMR; -; A=1-120.[ExPASy / RCSB / EBI]
PDBsum 1V49; -.
ModBase Q9GZQ8.
Protein-protein interaction databases
IntAct Q9GZQ8; -.
Organism-specific databases
H-InvDB HIX0013321; -.
HIX0035198; -.
HIX0059104; -.
HGNC HGNC:13352; MAP1LC3B.
GeneLynx MAP1LC3B; Homo sapiens.
GenAtlas MAP1LC3B.
MIM 609604; gene. [NCBI / EBI]
PharmGKB PA134923100; -.
GeneCards Q9GZQ8.
Gene expression databases
ArrayExpress Q9GZQ8; -.
CleanEx HS_MAP1LC3B; -.
GermOnline ENSG00000140941; Homo sapiens.
Ontologies
GO
GO:0005776; Cellular component: autophagic vacuole (inferred from sequence or structural similarity from UniProtKB).
GO:0031090; Cellular component: organelle membrane (inferred from sequence or structural similarity from UniProtKB).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from UniProtKB).
QuickGo view.
Family and domain databases
InterPro IPR004241; MAP1_LC3.
Graphical view of domain structure.
PANTHER PTHR10969; MAP1_LC3; 1.
Pfam PF02991; MAP1_LC3; 1.
Pfam graphical view of domain structure.
BLOCKS Q9GZQ8.
Genome annotation databases
Ensembl ENSG00000140941; Homo sapiens. [Contig view]
GeneID 81631; -.
KEGG hsa:81631; -.
Phylogenomic databases
HOGENOM Q9GZQ8; -.
HOVERGEN Q9GZQ8; -.
Other
LinkHub Q9GZQ8; -.
SOURCE MAP1LC3B; Homo sapiens.
ProtoNet Q9GZQ8.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Autophagy; Cytoplasm; Cytoplasmic vesicle; Lipoprotein; Membrane; Microtubule; Ubl conjugation pathway.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
INIT_MET   1     1        Removed (By similarity). 
CHAIN   2   120  119     Microtubule-associated proteins 1A/1B light chain 3B. PRO_0000017198
PROPEP   121   125  5     Removed in mature form. PRO_0000017199
LIPID   120   120        Phosphatidylethanolamine amidated glycine (Probable). 
MUTAGEN   120   120        G->A: No processing of precursor. 
MUTAGEN   122   122        K->A: No effect on processing of precursor. 
CONFLICT   61    61        S -> G (in Ref. 5; AAH67797). 
HELIX   7    10  4      
HELIX   13    26  14      
STRAND   30    37  8      
STRAND   50    55  6      
HELIX   60    70  11      
TURN   75    77  3      
STRAND   80    83  4      
HELIX   95   102  8      
STRAND   109   115  7      
Sequence information
Length: 125 AA [This is the length of the unprocessed precursor] Molecular weight: 14688 Da [This is the MW of the unprocessed precursor] CRC64: BB141DEC7653E83F [This is a checksum on the sequence]
        10         20         30         40         50         60 
MPSEKTFKQR RTFEQRVEDV RLIREQHPTK IPVIIERYKG EKQLPVLDKT KFLVPDHVNM 

        70         80         90        100        110        120 
SELIKIIRRR LQLNANQAFF LLVNGHSMVS VSTPISEVYE SEKDEDGFLY MVYASQETFG 


MKLSV 

Q9GZQ8 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

BLAST logo BLAST submission on ExPASy/SIB
or at NCBI (USA)
Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
PROSITE logo ScanProsite, MotifScan SWISS-MODEL Submit a homology modeling request to SWISS-MODEL
NPSA logo NPSA Sequence analysis tools

ExPASy logo ExPASy Home page Site Map Search ExPASy Contact us Swiss-Prot
 Hosted by ca flag CBR Canada Mirror sites: Australia  Brazil  China  Korea  Switzerland
Notice: This page will be replaced with www.uniprot.org. Please send us your feedback!