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UniProtKB/Swiss-Prot entry Q9CPX6


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name ATG3_MOUSE
Primary accession number Q9CPX6
Secondary accession number Q3TXJ9
Integrated into Swiss-Prot on March 15, 2005
Sequence was last modified on June 1, 2001 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 38)
Name and origin of the protein
Protein name Autophagy-related protein 3
Synonym APG3-like
Gene name
Name: Atg3
Synonyms: Apg3l
From
Mus musculus (Mouse) [TaxID: 10090] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus.
Protein existence 2: Evidence at transcript level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Brain;
Tanida I., Nishitani T., Tanida-Miyake E., Ueno T., Kominami E.;
"Murine Apg3p is an authentic E2 enzyme for Apg8p homologs.";
Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases.
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=C57BL/6J;
TISSUE=Bone marrow, Cerebellum, and Colon;
DOI=10.1126/science.1112014; PubMed=16141072 [NCBI, ExPASy, EBI, Israel, Japan]
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J., Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
"The transcriptional landscape of the mammalian genome.";
Science 309:1559-1563(2005).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=FVB/N;
TISSUE=Kidney;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
Comments
  • FUNCTION: GABARAPL1 (GABARAPL2 or GABARAP or MAP1LC3)-modifier protein conjugating enzyme involved in its E2-like covalent binding to PE. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3 (E2-like enzyme). Preferred substrate is MAP1LC3A. Formation of the GABARAPL1-PE conjugate is essential for autophagy (By similarity).
  • SUBUNIT: Interacts with ATG7 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5 (By similarity).
  • SUBCELLULAR LOCATION: Cytoplasm (By similarity).
  • SIMILARITY: Belongs to the ATG3 family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AB079386; BAC57452.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK005266; BAB23918.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK011409; BAB27600.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK078877; BAC37437.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK150914; BAE29952.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK159231; BAE34917.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC010809; AAH10809.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_080678.1; -.
UniGene Mm.41775
3D structure databases
ModBase Q9CPX6.
PTM databases
PhosphoSite Q9CPX6; -.
Organism-specific databases
MGI MGI:1915091; Atg3.
Gene expression databases
ArrayExpress Q9CPX6; -.
CleanEx MM_ATG3; -.
GermOnline ENSMUSG00000022663; Mus musculus.
Family and domain databases
InterPro IPR007135; Autophagy_C.
IPR007134; Autophagy_N.
Graphical view of domain structure.
Pfam PF03987; Autophagy_C; 1.
PF03986; Autophagy_N; 1.
Pfam graphical view of domain structure.
BLOCKS Q9CPX6.
Genome annotation databases
Ensembl ENSMUSG00000022663; Mus musculus. [Contig view]
GeneID 67841; -.
KEGG mmu:67841; -.
Phylogenomic databases
HOGENOM Q9CPX6; -.
HOVERGEN Q9CPX6; -.
Other
SOURCE Atg3; Mus musculus.
ProtoNet Q9CPX6.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Autophagy; Cytoplasm; Phosphoprotein; Protein transport; Transport; Ubl conjugation pathway.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   314  314     Autophagy-related protein 3. PRO_0000213570
ACT_SITE   264   264        Glycyl thioester intermediate (Potential). 
MOD_RES   18    18        Phosphotyrosine (By similarity). 
Sequence information
Length: 314 AA [This is the length of the unprocessed precursor] Molecular weight: 35796 Da [This is the MW of the unprocessed precursor] CRC64: EC5ECACD3247ED66 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MQNVINTVKG KALEVAEYLT PVLKESKFKE TGVITPEEFV AAGDHLVHHC PTWQWATGEE 

        70         80         90        100        110        120 
LKVKAYLPTD KQFLVTKNVP CYKRCKQMEY SDELEAIIEE DDGDGGWVDT YHNTGITGIT 

       130        140        150        160        170        180 
EAVKEITLES KDSIKLQDCS ALCDEEDEED EGEAADMEEY EESGLLETDE ATLDTRKIVE 

       190        200        210        220        230        240 
ACKAKADAGG EDAILQTRTY DLYITYDKYY QTPRLWLFGY DEQRQPLTVE HMYEDISQDH 

       250        260        270        280        290        300 
VKKTVTIENH PHLPPPPMCS VHPCRHAEVM KKIIETVAEG GGELGVHMYL LIFLKFVQAV 

       310 
IPTIEYDYTR HFTM 

Q9CPX6 in FASTA format

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