[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. STRAIN=Wistar; Maiorino M., Scapin M., Ursini F., Biasolo M., Bosello V., Flohe L.; "Distinct promoters determine alternative transcription of gpx-4 into PHGPx variants."; Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
[2]	NUCLEOTIDE SEQUENCE [MRNA]. STRAIN=Wistar; Tramer F., Vetere A., Martinelli M., Paroni F., Marsich E., Sandri G., Panfili E.; "Cloning of cDNA of rat phospholipid hydroperoxide/sperm nucleus glutathione peroxidase."; Submitted (MAR-2004) to the EMBL/GenBank/DDBJ databases.
[3]	NUCLEOTIDE SEQUENCE [MRNA] OF 1-250, AND SUBCELLULAR LOCATION. TISSUE=Testis; PubMed=11344099 [NCBI, ExPASy, EBI, Israel, Japan] Pfeifer H., Conrad M., Roethlein D., Kyriakopoulos A., Brielmeier M., Bornkamm G.W., Behne D.; "Identification of a specific sperm nuclei selenoenzyme necessary for protamine thiol cross-linking during sperm maturation."; FASEB J. 15:1236-1238(2001).

Comments

FUNCTION: Could play a major role in protecting mammals from the toxicity of ingested lipid hydroperoxides. Essential for embryonic development. Protects from radiation and oxidative damage. Stabilizes the condensed chromatin in sperm nuclei and is necessary male fertility (By similarity).
CATALYTIC ACTIVITY: 2 glutathione + a lipid hydroperoxide = glutathione disulfide + lipid + 2 H₂O.
SUBCELLULAR LOCATION: Nucleus.
ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative splicing.

Name Nuclear

Isoform ID Q91XR8-1

This is the isoform sequence displayed in this entry.

Name Mitochondrial

Isoform ID P36970-1

This isoform is stored in UniProtKB/Swiss-Prot entry P36970.
SIMILARITY: Belongs to the glutathione peroxidase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AJ537598; CAD61278.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AY570513; AAS76675.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF274028; AAK74113.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_001034938.1; -.

UniGene

Rn.3647

3D structure databases

HSSP

P00435; 1GP1. [HSSP ENTRY / PDB]

SMR

Q91XR8; 92-253.

ModBase

Q91XR8.

Protein family/group databases

PeroxiBase

3813; RnoGPx04-c.

Organism-specific databases

RGD

69226; Gpx4.

Ontologies

GO:0005634;	Cellular component: nucleus (inferred from sequence or structural similarity from UniProtKB).
GO:0004602;	Molecular function: glutathione peroxidase activity (inferred from sequence or structural similarity from UniProtKB).
GO:0006325;	Biological process: establishment or maintenance of chromatin architecture (inferred from sequence or structural similarity from UniProtKB).
GO:0007283;	Biological process: spermatogenesis (inferred from sequence or structural similarity from UniProtKB).
QuickGo view.

Family and domain databases

InterPro

IPR000889; Glut_peroxidase.
IPR012335; Thioredoxin_fold.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.30.10; Thioredoxin_fold; 1.

PANTHER

PTHR11592; Glut_peroxidase; 1.

Pfam

PF00255; GSHPx; 1.
Pfam graphical view of domain structure.

PRINTS

PR01011; GLUTPROXDASE.

PROSITE

PS00460; GLUTATHIONE_PEROXID_1; 1.
PS00763; GLUTATHIONE_PEROXID_2; 1.
PS51355; GLUTATHIONE_PEROXID_3; 1.
PROSITE graphical view of domain structure (profiles).

Genome annotation databases

GeneID

29328; -.

KEGG

rno:29328; -.

Phylogenomic databases

HOVERGEN

Q91XR8; -.

Other

NextBio

608793; -.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Alternative splicing; Developmental protein; Nucleus; Oxidoreductase; Peroxidase; Selenium; Selenocysteine.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 253`	`253`	`Phospholipid hydroperoxide glutathione peroxidase, nuclear.`	`PRO_0000066626`
`ACT_SITE`	`129 129`
`NON_STD`	`129 129`		`Selenocysteine.`
`CONFLICT`	`9 14`		`RGRCRQ -> PGRQCAGIRPYGP (in Ref. 3; AAK74113).`
`CONFLICT`	`101 101`		`A -> S (in Ref. 1; CAD61278).`
`CONFLICT`	`192 192`		`N -> S (in Ref. 2; AAS76675).`

Sequence information

Length: 253 AA [This is the length of the unprocessed precursor]

Molecular weight: 29335 Da [This is the MW of the unprocessed precursor]

CRC64: D108602E22DAD29A [This is a checksum on the sequence]

        10         20         30         40         50         60 
MGRAAARKRG RCRQRGRSPG GRRRREPGRQ SPRKRPGPRR RRARARRRRR ARPRRMEPIP 

        70         80         90        100        110        120 
EPFNPRPLLQ DLPQTSNSHE FLGLCASRDD WRCARSMHEF AAKDIDGHMV CLDKYRGCVC 

       130        140        150        160        170        180 
IVTNVASQUG KTDVNYTQLV DLHARYAECG LRILAFPCNQ FGRQEPGSNQ EIKEFAAGYN 

       190        200        210        220        230        240 
VRFDMYSKIC VNGDDAHPLW KWMKVQPKGR GMLGNAIKWN FTKFLIDKNG CVVKRYGPME 

       250 
EPQVIEKDLP CYL

Q91XR8 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools