PROTEIN SEQUENCE, AND MASS SPECTROMETRY.
TISSUE=Seed;
DOI=10.1002/jms.102; PubMed=11180645 [NCBI, ExPASy, EBI, Israel, Japan]
Di Maro A., Ferranti P., Mastronicola M., Polito L., Bolognesi A., Stirpe F., Malorni A., Parente A.;
"Reliable sequence determination of ribosome-inactivating proteins by combining electrospray mass spectrometry and Edman degradation.";
J. Mass Spectrom. 36:38-46(2001).

Comments

FUNCTION: Ribosome-inactivating protein of type 1, inhibits protein synthesis in animal cells (By similarity).
CATALYTIC ACTIVITY: Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.
MASS SPECTROMETRY: Mass=28495; Mass_error=2; Method=Electrospray; Range=1-253; Source=PubMed:11180645;.
SIMILARITY: Belongs to the ribosome-inactivating protein family. Type 1 RIP subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

PIR

A58923; A58923.

3D structure databases

HSSP

P23339; 1J1Q. [HSSP ENTRY / PDB]

SMR

Q7M1Z2; 1-253.

ModBase

Q7M1Z2.

Ontologies

GO:0006952;	Biological process: defense response (inferred from electronic annotation from UniProtKB-KW).
GO:0009405;	Biological process: pathogenesis (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR001574; Ribosome_inactivat_prot.
IPR016138; Ribosome_inactivat_prot_sub1.
IPR016139; Ribosome_inactivat_prot_sub2.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.420.10; Ribosome_inactivat_prot_sub1; 1.
G3DSA:4.10.470.10; Ribosome_inactivat_prot_sub2; 1.

Pfam

PF00161; RIP; 1.
Pfam graphical view of domain structure.

PRINTS

PR00396; SHIGARICIN.

PROSITE

PS00275; SHIGA_RICIN; FALSE_NEG.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Direct protein sequencing; Hydrolase; Plant defense; Protein synthesis inhibitor; Toxin.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 253`	`253`	`Ribosome-inactivating protein saporin-9.`	`PRO_0000253946`
`ACT_SITE`	`176 176`		`By similarity.`

Sequence information

Length: 253 AA [This is the length of the unprocessed precursor]

Molecular weight: 28494 Da [This is the MW of the unprocessed precursor]

CRC64: 22915D5494842389 [This is a checksum on the sequence]

        10         20         30         40         50         60 
VTSITLDLVN PTAGQYSSFV DKIRNNVKDP NLKYGGTDIA VIGPPSKDKF LRINFQSSRG 

        70         80         90        100        110        120 
TVSLGLKRDN LYVVAYLAMD NTNVNRAYYF RSEITSAELT ALFPEATAAN HKALEYTEDY 

       130        140        150        160        170        180 
HSIEKNAQIT EGDKSRKELG LGINLLSSTM DTVNKKVRVV KNEARFLLIA IQMTAEAVRF 

       190        200        210        220        230        240 
RYIQNLVTKN FPNKFNSENK VIKFEVNWKK ISTAIHGDAK NGVFNKDYDF GFGKVRLVKD 

       250 
LQMGLLMHLG KPK

Q7M1Z2 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools