NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 10895 / NRRL Y-1056 / CBS 109.51;
DOI=10.1126/science.1095781; PubMed=15001715 [NCBI, ExPASy, EBI, Israel, Japan]
Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P.;
"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome.";
Science 304:304-307(2004).

Comments

FUNCTION: Involved in ds DNA break repair. Has a role in non-homologous integration (NHI) pathways where it is required in the final step of non-homologus end-joining (By similarity).
CATALYTIC ACTIVITY: ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).
SUBCELLULAR LOCATION: Nucleus (By similarity).
SIMILARITY: Belongs to the ATP-dependent DNA ligase family.
SIMILARITY: Contains 2 BRCT domains.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AE016816; AAS51235.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_983411.1; -.

3D structure databases

ModBase

Q75CA4.

Organism-specific databases

AGD

ACR008W; -.

Ontologies

GO:0005634;	Cellular component: nucleus (inferred from electronic annotation from UniProtKB-KW).
GO:0005524;	Molecular function: ATP binding (inferred from electronic annotation from InterPro).
GO:0003910;	Molecular function: DNA ligase (ATP) activity (inferred from electronic annotation from InterPro).
GO:0006310;	Biological process: DNA recombination (inferred from electronic annotation from InterPro).
GO:0006281;	Biological process: DNA repair (inferred from electronic annotation from InterPro).
GO:0006260;	Biological process: DNA replication (inferred from electronic annotation from InterPro).
QuickGo view.

Family and domain databases

InterPro

IPR001357; BRCT.
IPR000977; DNA_ligase.
IPR012310; DNA_ligase_A_M.
IPR016059; DNA_ligase_CS.
IPR012340; NA-bd_OB-fold.
Graphical view of domain structure.

Gene3D

G3DSA:2.40.50.140; OB_NA_bd_sub; 1.

Pfam

PF00533; BRCT; 2.
PF01068; DNA_ligase_A_M; 1.
Pfam graphical view of domain structure.

SMART

SM00292; BRCT; 2.
SMART graphical view of domain structure.

TIGRFAMs

TIGR00574; dnl1; 1.

PROSITE

PS50172; BRCT; 2.
PS00697; DNA_LIGASE_A1; 1.
PS50160; DNA_LIGASE_A3; 1.
PROSITE graphical view of domain structure (profiles).

ProtoNet

Q75CA4.

Genome annotation databases

4619536; -.

fig|33169.1.peg.1056; -.

Phylogenomic databases

HOGENOM

Q75CA4; -.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

ATP-binding; Complete proteome; DNA damage; DNA recombination; DNA repair; DNA replication; Ligase; Nucleotide-binding; Nucleus; Repeat.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 981`	`981`	`DNA ligase 4.`	`PRO_0000278374`
`DOMAIN`	`721 819`	`99`	`BRCT 1.`
`DOMAIN`	`875 980`	`106`	`BRCT 2.`
`ACT_SITE`	`322 322`		`N6-AMP-lysine intermediate (By similarity).`

Sequence information

Length: 981 AA [This is the length of the unprocessed precursor]

Molecular weight: 111881 Da [This is the MW of the unprocessed precursor]

CRC64: 9883B3EEDACA8A25 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MVIYPWLKNT TELTIALPKK PIIHANMDVL GSPRGGTPTG EHEINAQDGS PINFSPSPDF 

        70         80         90        100        110        120 
CWLCDELFIK LEEVALKKKD LGKPRKVRNL EITSNFVSLW RKTVGNDIYP ALVLSLPYND 

       130        140        150        160        170        180 
RRSYRVKDVT LVKALCKHMK LPRNSETERR LLHWKQNAPR GVKLSTFCVE ELQKRRREPV 

       190        200        210        220        230        240 
VPKRMSIDEV NGMLDKLEHE SNVGKWSYIS LAESPAFNYC LEHMSYVELR FFFDIVLKVP 

       250        260        270        280        290        300 
IVSGLESLLL SCWHPDAESY FKVVSDLRIV AHTLYDPNER LEKNDLSVRI GYAFAPHMAQ 

       310        320        330        340        350        360 
RVKIPYEKVS TKLGNDFYVE EKMDGDRIQV HYMDYGNSIA YFSRNGINYT YLYGENSSKG 

       370        380        390        400        410        420 
SISNHLKFVE GVKECILDGE MVSYDKEMQC ILPFGLTKSG ASHQVNFETT GHTEPTYRPL 

       430        440        450        460        470        480 
YAVFDLLYLN GQLLTNQDVV KRKEYLEKIL IPSKNVVHLL SGPRCSDAEA ITAALGAAVA 

       490        500        510        520        530        540 
HGSEGIVLKK ARSKYSVGKR DDSWIKIKPE YLENFGENMD LVVIGRDKGR KDSFICALAV 

       550        560        570        580        590        600 
TDDSEKNNPS SYESGSDSDS DSEPIIVQPK IEKFISFCSI ANGISNEEFK EIDRLTRGNW 

       610        620        630        640        650        660 
FPYDERKPPT DWVEFGTKTP REWIDPKNSV VLEVKARSID NEESKSDLYK TGSTLYNAYC 

       670        680        690        700        710        720 
KRIRHDKNWS TASTVAEYDT AREARSYFNV SQNAKFGKDR SSPRKRRTFH LVGDIDVTKP 

       730        740        750        760        770        780 
SKADFLKGYY FYVTSGYFDL QSKKNIDASE IGEAVVSCGG TYIHNLRIRA SLDKLYILGC 

       790        800        810        820        830        840 
KDTRELKMLI ERGYDIIHPE WLMDCVKYGT MLQIEPKYVY SASEELMKQA RNQEDKYGES 

       850        860        870        880        890        900 
YQLPVTEDTL KALANKQVEE GYASEMGTDA VSEYERLLIF KGWLFYILDD YAYHSSWSDI 

       910        920        930        940        950        960 
VKWNIESCGG EVTNDLELAT IVVAVKDCFS QLSLQAVRNN IGARITGSND VQPIPKIVTS 

       970        980 
EWVEACMEAQ YLVDEDEYAA I

Q75CA4 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools