[1]	NUCLEOTIDE SEQUENCE [MRNA]. Laser M., Li Y., Xu L., Darden A., Wu B.X., Hazard E.S. III, Crosson C., Ma J.X.; "Identification and characterization of a novel gene induced by ischemic preconditioning in the retina."; Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases.
[2]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. TISSUE=Testis; DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan] The MGC Project Team; "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."; Genome Res. 14:2121-2127(2004).

Comments

FUNCTION: GABARAPL1 (GABARAPL2 or GABARAP or MAP1LC3)-modifier protein conjugating enzyme involved in its E2-like covalent binding to PE. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3 (E2-like enzyme). Preferred substrate is MAP1LC3A. Formation of the GABARAPL1-PE conjugate is essential for autophagy (By similarity).
SUBUNIT: Interacts with ATG7 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5 (By similarity).
SUBCELLULAR LOCATION: Cytoplasm (By similarity).
SIMILARITY: Belongs to the ATG3 family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AF175224; AAG09182.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BC078743; AAH78743.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

IPI00191959; -.

NP_599221.1; -.

3D structure databases

ModBase

Q6AZ50.

Organism-specific databases

RGD

708464; Atg3.

Gene expression databases

ArrayExpress

Q6AZ50; -.

GermOnline

ENSRNOG00000002094; Rattus norvegicus.

Ontologies

GO:0005829;	Cellular component: cytosol (inferred from direct assay from RGD).
GO:0006914;	Biological process: autophagy (inferred from expression pattern from RGD).
GO:0019941;	Biological process: modification-dependent protein catabolic process (inferred from electronic annotation from UniProtKB-KW).
GO:0015031;	Biological process: protein transport (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR007135; Autophagy-rel_prot_3.
IPR019461; Autophagy-rel_prot_3_C.
IPR007134; Autophagy-rel_prot_3_N.
Graphical view of domain structure.

Pfam

PF03987; Autophagy_act_C; 1.
PF10381; Autophagy_Cterm; 1.
PF03986; Autophagy_N; 1.
Pfam graphical view of domain structure.

Genome annotation databases

Ensembl

ENSRNOG00000002094; Rattus norvegicus. [Contig view]

GeneID

171415; -.

KEGG

rno:171415; -.

Phylogenomic databases

HOVERGEN

Q6AZ50; -.

OMA

Q6AZ50; YHNTGIA.

Other

622307; -.

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Autophagy; Cytoplasm; Phosphoprotein; Protein transport; Transport; Ubl conjugation pathway.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 314`	`314`	`Autophagy-related protein 3.`	`PRO_0000213571`
`ACT_SITE`	`264 264`		`Glycyl thioester intermediate (Potential).`
`MOD_RES`	`18 18`		`Phosphotyrosine (By similarity).`
`CONFLICT`	`257 257`		`P -> L (in Ref. 1; AAG09182).`

Sequence information

Length: 314 AA [This is the length of the unprocessed precursor]

Molecular weight: 35822 Da [This is the MW of the unprocessed precursor]

CRC64: 4C78AB0F628C9BDF [This is a checksum on the sequence]

        10         20         30         40         50         60 
MQNVINTVKG KALEVAEYLT PVLKESKFKE TGVITPEEFV AAGDHLVHHC PTWQWATGEE 

        70         80         90        100        110        120 
LKVKAYLPTG KQFLVTKNVP CYKRCKQMEY SDELEAIIEE DDGDGGWVDT YHNTGITGIT 

       130        140        150        160        170        180 
EAVKEITLES KDSIKLQDCS VLCDEEEEEE EGEAADMEEY EESGLLETDE ATLDTRRIVE 

       190        200        210        220        230        240 
ACKAKADAGG EDAILQTRTY DLYITYDKYY QTPRLWLFGY DEQRQPLTVE HMYEDISQDH 

       250        260        270        280        290        300 
VKKTVTIENH PHLPPPPMCS VHPCRHAEVM KKIIETVAEG GGELGVHMYL LIFLKFVQAV 

       310 
IPTIEYDYTR HFTM

Q6AZ50 in FASTA format

View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools