[1]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. TISSUE=Testis; DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan] The MGC Project Team; "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."; Genome Res. 14:2121-2127(2004).
[2]	TISSUE SPECIFICITY. DOI=10.1006/bbrc.2002.6645; PubMed=11890701 [NCBI, ExPASy, EBI, Israel, Japan] Tanida I., Tanida-Miyake E., Nishitani T., Komatsu M., Yamazaki H., Ueno T., Kominami E.; "Murine Apg12p has a substrate preference for murine Apg7p over three Apg8p homologs."; Biochem. Biophys. Res. Commun. 292:256-262(2002).

Comments

FUNCTION: Functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. Forms intermediate conjugates with GABARAPL1 (GABARAPL2, GABARAP or MAP1ALC3). Formation of the final GABARAPL1-PE conjugate is essential for autophagy (By similarity).
SUBUNIT: Homodimer. Interacts with ATG3 and ATG12. The complex, composed of APG3L and APG7L, plays a role in the conjugation of ATG12 to ATG5 (By similarity).
SUBCELLULAR LOCATION: Cytoplasm (Probable).
TISSUE SPECIFICITY: Widely expressed.
DOMAIN: The C-terminal part of the protein is essential for the dimerization and interaction with ATG3 and ATG12 (By similarity).
SIMILARITY: Belongs to the ATG7 family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

BC082059; AAH82059.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_001012097.1; -.

UniGene

Rn.162765

3D structure databases

ModBase

Q641Y5.

Organism-specific databases

RGD

1304817; Atg7.

Family and domain databases

InterPro

IPR006285; E1_like_Apg7.
IPR016040; NAD(P)-bd.
IPR000594; ThiF_NAD_FAD_bd.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.50.720; NAD(P)-bd; 1.

Pfam

PF00899; ThiF; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR01381; E1_like_apg7; 1.

BLOCKS

Q641Y5.

Genome annotation databases

Ensembl

ENSRNOG00000007486; Rattus norvegicus. [Contig view]

GeneID

312647; -.

KEGG

rno:312647; -.

Phylogenomic databases

HOVERGEN

Q641Y5; -.

Other

ProtoNet

Q641Y5.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Autophagy; Cytoplasm; Protein transport; Transport; Ubl conjugation pathway.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 698`	`698`	`Autophagy-related protein 7.`	`PRO_0000212808`
`ACT_SITE`	`567 567`		`Glycyl thioester intermediate (Potential).`

Sequence information

Length: 698 AA [This is the length of the unprocessed precursor]

Molecular weight: 77436 Da [This is the MW of the unprocessed precursor]

CRC64: B26001CB18054498 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MGDPGLSKLQ FAPFNSALDV GFWHELTQKK LNEYRLDEAP KDIKGYYYNG DSAGLPTRLT 

        70         80         90        100        110        120 
LEFSAFDMSA PTPARCCPAM GTLHNTNTLE AFKTADKKLL LEQSANEIWE AIKSGAALEN 

       130        140        150        160        170        180 
PMLLNKFLLL TFADLKKYHF YYWFCCPALC LPESIPLIRG PVGLDQRLSP KQIQALEHAY 

       190        200        210        220        230        240 
DDLCRTEGVT ALPYFLFKYD DDTVLVSLLK HYSDFFQGQR TKLTVGVYDP CNLTQHPGWP 

       250        260        270        280        290        300 
LRNFLVLAAH RWSGSFQSVE VLCFRDRTMQ GARDVTHSII FEVKLPEMAF SPDCPKAVGW 

       310        320        330        340        350        360 
EKNQKGGMGP RMVNLSGCMD PKRLAESSVD LNLKLMCWRL VPTLDLDKVV SVKCLLLGAG 

       370        380        390        400        410        420 
TLGCNVARTL MGWGVRHVTF VDNAKISYSN PVRQPLYEFE DCLGGGKPKA LAAAERLQKI 

       430        440        450        460        470        480 
FPGVNASGFN MSIPMPGHPV NFSDVTMEQA RRDVEQLEEL IDSHDVIFLL MDTRESRWLP 

       490        500        510        520        530        540 
TVIAASKRKL VINAALGFDT FVVMRHGLKK PKQQGAGDLC PSHLVAPADL GSSLFANIPG 

       550        560        570        580        590        600 
YKLGCYFCND VVAPGDSTRD RTLDQQCTVS RPGLAVIAGA LAVELMVSVL QHPEGGYAIA 

       610        620        630        640        650        660 
SSSDDRMNEP PTSLGLVPHQ IRGFLSRFDN VLPVSLAFDK CTACSSKVLD QYEQEGFTFL 

       670        680        690 
AKVFNSSHSF LEDLTGLTLL HQETQAAEIW DMSDEETV

Q641Y5 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools