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UniProtKB/Swiss-Prot entry Q62666


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name VACHT_RAT
Primary accession number Q62666
Secondary accession number Q64351
Integrated into Swiss-Prot on September 19, 2003
Sequence was last modified on September 19, 2003 (Sequence version 2)
Annotations were last modified on    May 5, 2009 (Entry version 60)
Name and origin of the protein
Protein name Vesicular acetylcholine transporter
Synonyms VAChT
rVAT
Solute carrier family 18 member 3
Gene name
Name: Slc18a3
Synonyms: Vacht, Vat
From
Rattus norvegicus (Rat) [TaxID: 10116] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
Protein existence 2: Evidence at transcript level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
TISSUE=Brain stem;
DOI=10.1073/pnas.91.22.10620; PubMed=7938002 [NCBI, ExPASy, EBI, Israel, Japan]
Roghani A., Feldman J., Kohan S.A., Shirzadi A., Gundersen C.B., Brecha N., Edwards R.H.;
"Molecular cloning of a putative vesicular transporter for acetylcholine.";
Proc. Natl. Acad. Sci. U.S.A. 91:10620-10624(1994).
[2]
NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
TISSUE=Pheochromocytoma;
PubMed=8071310 [NCBI, ExPASy, EBI, Israel, Japan]
Erickson J.D., Varoqui H., Schafer M.K.-H., Modi W., Diebler M.-F., Weihe E., Rand J.B., Eiden L.E., Bonner T.I., Usdin T.B.;
"Functional identification of a vesicular acetylcholine transporter and its expression from a 'cholinergic' gene locus.";
J. Biol. Chem. 269:21929-21932(1994).
[3]
NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND TISSUE SPECIFICITY.
STRAIN=Sprague-Dawley;
TISSUE=Spinal cord;
PubMed=8071313 [NCBI, ExPASy, EBI, Israel, Japan]
Bejanin S., Cervini R., Mallet J., Berrard S.;
"A unique gene organization for two cholinergic markers, choline acetyltransferase and a putative vesicular transporter of acetylcholine.";
J. Biol. Chem. 269:21944-21947(1994).
Comments
  • FUNCTION: Involved in acetylcholine transport into synaptic vesicles.
  • SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
  • TISSUE SPECIFICITY: High expression in all major cholinergic cell groups, including peripheral postganglionic parasympathetic cells, preganglionic sympathetic and parasympathetic cells, ventral spinal cord and brainstem motoneurons, cell groups in the basal forebrain, the habenula and the corpus striatum. Weakly expressed in the cortex and hippocampus.
  • SIMILARITY: Belongs to the major facilitator superfamily. Vesicular transporter family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
U09838; AAA50831.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
U09211; AAA20498.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X80395; CAA56604.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
IPI IPI00326574; -.
RefSeq NP_113851.1; -.
UniGene Rn.9987
3D structure databases
ModBase Q62666.
PTM databases
PhosphoSite Q62666; -.
Organism-specific databases
RGD 62072; Slc18a3.
Gene expression databases
ArrayExpress Q62666; -.
GermOnline ENSRNOG00000025008; Rattus norvegicus.
Ontologies
GO
GO:0016021; Cellular component: integral to membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0006836; Biological process: neurotransmitter transport (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR011701; MFS_1.
Graphical view of domain structure.
Pfam PF07690; MFS_1; 1.
Pfam graphical view of domain structure.
PROSITE PS50850; MFS; 1.
PROSITE graphical view of domain structure (profiles).
Genome annotation databases
Ensembl ENSRNOG00000025008; Rattus norvegicus. [Contig view]
GeneID 60422; -.
KEGG rno:60422; -.
Phylogenomic databases
HOVERGEN Q62666; -.
Other
NextBio 612138; -.
ProtoNet Q62666.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Glycoprotein; Membrane; Neurotransmitter transport; Transmembrane; Transport.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   530  530     Vesicular acetylcholine transporter. PRO_0000127520
TOPO_DOM   1    33  33     Cytoplasmic (Potential). 
TRANSMEM   34    54  21     Potential. 
TOPO_DOM   55   125  71     Lumenal, vesicle (Potential). 
TRANSMEM   126   146  21     Potential. 
TOPO_DOM   147   152  6     Cytoplasmic (Potential). 
TRANSMEM   153   173  21     Potential. 
TOPO_DOM   174   182  9     Lumenal, vesicle (Potential). 
TRANSMEM   183   203  21     Potential. 
TOPO_DOM   204   213  10     Cytoplasmic (Potential). 
TRANSMEM   214   234  21     Potential. 
TOPO_DOM   235   242  8     Lumenal, vesicle (Potential). 
TRANSMEM   243   263  21     Potential. 
TOPO_DOM   264   289  26     Cytoplasmic (Potential). 
TRANSMEM   290   310  21     Potential. 
TOPO_DOM   311   325  15     Lumenal, vesicle (Potential). 
TRANSMEM   326   346  21     Potential. 
TOPO_DOM   347   356  10     Cytoplasmic (Potential). 
TRANSMEM   357   377  21     Potential. 
TOPO_DOM   378   388  11     Lumenal, vesicle (Potential). 
TRANSMEM   389   409  21     Potential. 
TOPO_DOM   410   422  13     Cytoplasmic (Potential). 
TRANSMEM   423   443  21     Potential. 
TOPO_DOM   444   447  4     Lumenal, vesicle (Potential). 
TRANSMEM   448   468  21     Potential. 
TOPO_DOM   469   530  62     Cytoplasmic (Potential). 
CARBOHYD   89    89        N-linked (GlcNAc...) (Potential). 
CARBOHYD   96    96        N-linked (GlcNAc...) (Potential). 
CONFLICT   92    92        A -> T (in Ref. 1; AAA50831). 
Sequence information
Length: 530 AA [This is the length of the unprocessed precursor] Molecular weight: 56537 Da [This is the MW of the unprocessed precursor] CRC64: 07720DA749527438 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MEPTAPTGQA RAAATKLSEA VGAALQEPQR QRRLVLVIVC VALLLDNMLY MVIVPIVPDY 

        70         80         90        100        110        120 
IAHMRGGSEG PTLVSEVWEP TLPPPTLANA SAYLANTSAS PTAAGSARSI LRPRYPTESE 

       130        140        150        160        170        180 
DVKIGVLFAS KAILQLLVNP LSGPFIDRMS YDVPLLIGLG VMFASTVMFA FAEDYATLFA 

       190        200        210        220        230        240 
ARSLQGLGSA FADTSGIAMI ADKYPEEPER SRALGVALAF ISFGSLVAPP FGGILYEFAG 

       250        260        270        280        290        300 
KRVPFLVLAA VSLFDALLLL AVAKPFSAAA RARANLPVGT PIHRLMLDPY IAVVAGALTT 

       310        320        330        340        350        360 
CNIPLAFLEP TIATWMKHTM AASEWEMGMV WLPAFVPHVL GVYLTVRLAA RYPHLQWLYG 

       370        380        390        400        410        420 
ALGLAVIGVS SCVVPACRSF APLVVSLCGL CFGIALVDTA LLPTLAFLVD VRHVSVYGSV 

       430        440        450        460        470        480 
YAIADISYSV AYALGPIVAG HIVHSLGFEQ LSLGMGLANL LYAPVLLLLR NVGLLTRSRS 

       490        500        510        520        530 
ERDVLLDEPP QGLYDAVRLR EVQGKDGGEP CSPPGPFDGC EDDYNYYSRS 

Q62666 in FASTA format

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