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UniProtKB/Swiss-Prot entry Q62277


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name SYPH_MOUSE
Primary accession number Q62277
Secondary accession numbers Q8BRQ0 Q91WI8
Integrated into Swiss-Prot on November 1, 1997
Sequence was last modified on October 10, 2003 (Sequence version 2)
Annotations were last modified on    June 16, 2009 (Entry version 71)
Name and origin of the protein
Protein name Synaptophysin
Synonyms Major synaptic vesicle protein p38
BM89 antigen
Gene name
Name: Syp
From
Mus musculus (Mouse) [TaxID: 10090] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=C57BL/6J;
TISSUE=Brain cortex;
DOI=10.1126/science.1112014; PubMed=16141072 [NCBI, ExPASy, EBI, Israel, Japan]
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J., Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
"The transcriptional landscape of the mammalian genome.";
Science 309:1559-1563(2005).
[2]
NUCLEOTIDE SEQUENCE [MRNA] OF 7-314.
STRAIN=C57BL/6;
TISSUE=Brain;
DOI=10.1002/(SICI)1097-4547(19970615)48:6<507::AID-JNR3>3.3.CO;2-2; PubMed=9210520 [NCBI, ExPASy, EBI, Israel, Japan]
Gaitanou M., Mamalaki A., Merkouri E., Matsas R.;
"Purification and cDNA cloning of mouse BM89 antigen shows that it is identical with the synaptic vesicle protein synaptophysin.";
J. Neurosci. Res. 48:507-514(1997).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 7-314.
TISSUE=Eye;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[4]
PROTEIN SEQUENCE OF 77-89; 170-179 AND 226-235, AND MASS SPECTROMETRY.
STRAIN=C57BL/6;
TISSUE=Brain;
Lubec G., Kang S.U.;
Submitted (APR-2007) to UniProtKB.
[5]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-81, AND MASS SPECTROMETRY.
TISSUE=Brain;
DOI=10.1021/pr0701254; PubMed=18034455 [NCBI, ExPASy, EBI, Israel, Japan]
Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.;
"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain.";
J. Proteome Res. 7:311-318(2008).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AK043756; BAC31642.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X95818; CAA65084.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC014823; AAH14823.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
IPI IPI00123505; -.
RefSeq NP_033331.2; -.
UniGene Mm.223674
3D structure databases
ModBase Q62277.
Protein-protein interaction databases
IntAct Q62277; 2.
PTM databases
PhosphoSite Q62277; -.
Organism-specific databases
MGI MGI:98467; Syp.
Gene expression databases
ArrayExpress Q62277; -.
Bgee Q62277; -.
CleanEx MM_SYP; -.
GermOnline ENSMUSG00000031144; Mus musculus.
Ontologies
GO
GO:0030054; Cellular component: cell junction (inferred from electronic annotation from UniProtKB-SubCell).
GO:0030285; Cellular component: integral to synaptic vesicle membrane (traceable author statement from MGI).
GO:0042734; Cellular component: presynaptic membrane (inferred from direct assay from MGI).
GO:0030141; Cellular component: secretory granule (inferred from electronic annotation from UniProtKB-SubCell).
GO:0019717; Cellular component: synaptosome (inferred from direct assay from MGI).
GO:0005509; Molecular function: calcium ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0005215; Molecular function: transporter activity (inferred from electronic annotation from InterPro).
GO:0006897; Biological process: endocytosis (traceable author statement from MGI).
GO:0007268; Biological process: synaptic transmission (traceable author statement from MGI).
QuickGo view.
Family and domain databases
InterPro IPR008253; Marvel.
IPR001285; Synaptophysin/porin.
IPR015457; Synaptophysin_.
Graphical view of domain structure.
PANTHER PTHR10306; Synaptophysin; 1.
PTHR10306:SF2; Synaptophysin_; 1.
Pfam PF01284; MARVEL; 1.
Pfam graphical view of domain structure.
PRINTS PR00220; SYNAPTOPHYSN.
PROSITE PS51225; MARVEL; 1.
PS00604; SYNAPTOP; 1.
PROSITE graphical view of domain structure (profiles).
Proteomic databases
PRIDE Q62277; -.
Genome annotation databases
Ensembl ENSMUSG00000031144; Mus musculus. [Contig view]
GeneID 20977; -.
KEGG mmu:20977; -.
Phylogenomic databases
HOGENOM Q62277; -.
HOVERGEN Q62277; -.
OMA Q62277; TGNTCKE.
Other
NextBio 299960; -.
SOURCE Syp; Mus musculus.
ProtoNet Q62277.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Calcium; Cell junction; Cytoplasmic vesicle; Direct protein sequencing; Glycoprotein; Membrane; Phosphoprotein; Repeat; Synapse; Synaptosome; Transmembrane; Ubl conjugation.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   314  314     Synaptophysin. PRO_0000179162
TOPO_DOM   1    25  25     Cytoplasmic (Potential). 
TRANSMEM   26    49  24     Potential. 
TOPO_DOM   50   107  58     Vesicular (Potential). 
TRANSMEM   108   131  24     Potential. 
TOPO_DOM   132   138  7     Cytoplasmic (Potential). 
TRANSMEM   139   162  24     Potential. 
TOPO_DOM   163   200  38     Vesicular (Potential). 
TRANSMEM   201   224  24     Potential. 
TOPO_DOM   225   314  90     Cytoplasmic (Potential). 
DOMAIN   21   228  208     MARVEL. 
REGION   255   305  51     Repeats, Gly-rich. 
MOD_RES   81    81        Phosphotyrosine. 
MOD_RES   227   227        Phosphothreonine (Potential). 
MOD_RES   279   279        Phosphotyrosine (Potential). 
MOD_RES   296   296        Phosphotyrosine (Potential). 
CARBOHYD   59    59        N-linked (GlcNAc...) (Potential). 
CONFLICT   105   105        A -> G (in Ref. 2; CAA65084). 
Sequence information
Length: 314 AA [This is the length of the unprocessed precursor] Molecular weight: 34025 Da [This is the MW of the unprocessed precursor] CRC64: F6AC6E30AECE10CE [This is a checksum on the sequence]
        10         20         30         40         50         60 
MLLLADMDVV NQLVAGGQFR VVKEPLGFVK VLQWVFAIFA FATCGSYTGE LRLSVECANK 

        70         80         90        100        110        120 
TESALNIEVE FEYPFRLHQV YFDAPSCVKG GTTKIFLVGD YSSSAEFFVT VAVFAFLYSM 

       130        140        150        160        170        180 
GALATYIFLQ NKYRENNKGP MMDFLATAVF AFMWLVSSSA WAKGLSDVKM ATDPENIIKE 

       190        200        210        220        230        240 
MPMCRQTGNT CKELRDPVTS GLNTSVVFGF LNLVLWVGNL WFVFKETGWA APFMRAPPGA 

       250        260        270        280        290        300 
PEKQPAPGDA YGDAGYGQGP GGYGPQDSYG PQGGYQPDYG QPASGGGGGY GPQGDYGQQG 

       310 
YGQQGAPTSF SNQM 

Q62277 in FASTA format

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