NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-15.
STRAIN=ATCC 43587 / DSM 3638 / JCM 8422 / Vc1;
PubMed=8550425 [NCBI, ExPASy, EBI, Israel, Japan]
Kletzin A., Adams M.W.W.;
"Molecular and phylogenetic characterization of pyruvate and 2-ketoisovalerate ferredoxin oxidoreductases from Pyrococcus furiosus and pyruvate ferredoxin oxidoreductase from Thermotoga maritima.";
J. Bacteriol. 178:248-257(1996).

[2]

NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 43587 / DSM 3638 / JCM 8422 / Vc1;
Weiss R.B., Dunn D.M., Robb F.T., Brown J.R.;
"The complete sequence of the Pyrococcus furiosus genome.";
Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases.

Comments

CATALYTIC ACTIVITY: 3-methyl-2-oxobutanoate + CoA + 2 oxidized ferredoxin = S-(2-methylpropanoyl)-CoA + CO₂ + 2 reduced ferredoxin + H⁺.
COFACTOR: Binds 2 4Fe-4S clusters (By similarity).
SUBUNIT: Heterotetramer of one alpha, one beta, one delta and one gamma chain.
SIMILARITY: Contains 2 4Fe-4S ferredoxin-type domains.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X85250; CAA59501.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AE010209; AAL81094.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

T45084; T45084.

RefSeq

NP_578699.1; -.

3D structure databases

HSSP

P00198; 2FDN. [HSSP ENTRY / PDB]

ModBase

Q51800.

Ontologies

GO:0043807;	Molecular function: 3-methyl-2-oxobutanoate dehydrogenase (ferredoxin) activity (inferred from electronic annotation from EC).
GO:0005506;	Molecular function: iron ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0022900;	Biological process: electron transport chain (inferred from electronic annotation from UniProtKB-KW).
GO:0006810;	Biological process: transport (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR001450; 4Fe4S_Fe_S_bd.
IPR011898; PorD_KorD.
Graphical view of domain structure.

Pfam

PF00037; Fer4; 2.
Pfam graphical view of domain structure.

PRINTS

PR00353; 4FE4SFRDOXIN.

TIGRFAMs

TIGR02179; PorD_KorD; 1.

PROSITE

PS00198; 4FE4S_FER_1; 2.
PS51379; 4FE4S_FER_2; 2.
PROSITE graphical view of domain structure (profiles).

Genome annotation databases

1468835; -.

AE009950_GR; PF0970.

pfu:PF0970; -.

fig|186497.1.peg.1006; -.

Phylogenomic databases

HOGENOM

Q51800; -.

Genome annotation databases

CMR

Q51800; PF0970.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

4Fe-4S; Complete proteome; Direct protein sequencing; Electron transport; Iron; Iron-sulfur; Metal-binding; Oxidoreductase; Repeat; Transport.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 105`	`105`	`Ketoisovalerate oxidoreductase subunit vorD.`	`PRO_0000099962`
`DOMAIN`	`44 73`	`30`	`4Fe-4S ferredoxin-type 1.`
`DOMAIN`	`74 103`	`30`	`4Fe-4S ferredoxin-type 2.`
`METAL`	`53 53`		`Iron-sulfur 1 (4Fe-4S) (Potential).`
`METAL`	`56 56`		`Iron-sulfur 1 (4Fe-4S) (Potential).`
`METAL`	`59 59`		`Iron-sulfur 1 (4Fe-4S) (Potential).`
`METAL`	`63 63`		`Iron-sulfur 2 (4Fe-4S) (Potential).`
`METAL`	`83 83`		`Iron-sulfur 2 (4Fe-4S) (Potential).`
`METAL`	`86 86`		`Iron-sulfur 2 (4Fe-4S) (Potential).`
`METAL`	`89 89`		`Iron-sulfur 2 (4Fe-4S) (Potential).`
`METAL`	`93 93`		`Iron-sulfur 1 (4Fe-4S) (Potential).`

Sequence information

Length: 105 AA [This is the length of the unprocessed precursor]

Molecular weight: 11851 Da [This is the MW of the unprocessed precursor]

CRC64: F609078E069DB899 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MNTLFGKTKE EAKPIVLKSV DEYPEAPISL GTTLVNPTGD WRTFKPVVNE EKCVKCYICW 

        70         80         90        100 
KYCPEPAIYI KPDGYVAIDY DYCKGCGICA NECPTKAITM IKEEK

Q51800 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools