FUNCTION: Catalytic subunit of the periplasmic nitrate reductase (NAP). Only expressed at high levels during aerobic growth. NapAB complex receives electrons from the membrane-anchored tetraheme protein napC, thus allowing electron flow between membrane and periplasm. Essential function for nitrate assimilation and may have a role in anaerobic metabolism (By similarity).
CATALYTIC ACTIVITY: Nitrite + acceptor = nitrate + reduced acceptor.
COFACTOR: Binds 1 4Fe-4S cluster (By similarity).
COFACTOR: Binds 1 molybdenum ion per subunit (By similarity).
COFACTOR: Binds 2 molybdopterin guanine dinucleotide (MGD) groups per subunit (By similarity).
SUBUNIT: Interacts with napB (By similarity).
SUBCELLULAR LOCATION: Periplasm (By similarity).
PTM: Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
SIMILARITY: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family. NasA/napA/narB subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

CP000282; ABD79348.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_525560.1; -.

3D structure databases

SMR

Q21PN1; 43-830.

ModBase

Q21PN1.

Enzyme and pathway databases

BioCyc

SDEG203122:SDE_0084-MON; -.

Ontologies

GO:0042597;	Cellular component: periplasmic space (inferred from electronic annotation from UniProtKB-KW).
GO:0009055;	Molecular function: electron carrier activity (inferred from electronic annotation from HAMAP).
GO:0005506;	Molecular function: iron ion binding (inferred from electronic annotation from HAMAP).
GO:0008940;	Molecular function: nitrate reductase activity (inferred from electronic annotation from HAMAP).
GO:0022900;	Biological process: electron transport chain (inferred from electronic annotation from UniProtKB-KW).
GO:0006777;	Biological process: Mo-molybdopterin cofactor biosynthetic process (inferred from electronic annotation from HAMAP).
GO:0042128;	Biological process: nitrate assimilation (inferred from electronic annotation from HAMAP).
GO:0006810;	Biological process: transport (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

HAMAP

MF_01630; -; 1.
PBIL [Tree]

InterPro

IPR009010; Asp_de-COase-like_fold.
IPR006656; Mopterin_OxRdtase.
IPR006963; Mopterin_OxRdtase_Fe4S4.
IPR006655; Mopterin_OxRdtase_prok_CS.
IPR006657; MPT_dinuc_bd.
IPR010051; NO3_reductase_lsu_periplasm.
IPR006311; Tat.
Graphical view of domain structure.

Gene3D

G3DSA:2.40.40.20; Asp_decarboxylase-like_fold; 1.

Pfam

PF04879; Molybdop_Fe4S4; 1.
PF00384; Molybdopterin; 1.
PF01568; Molydop_binding; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR01706; NAPA; 1.
TIGR01409; TAT_signal_seq; 1.

PROSITE

PS00551; MOLYBDOPTERIN_PROK_1; 1.
PS00490; MOLYBDOPTERIN_PROK_2; FALSE_NEG.
PS00932; MOLYBDOPTERIN_PROK_3; FALSE_NEG.
PS51318; TAT; 1.
PROSITE graphical view of domain structure (profiles).

Genome annotation databases

GeneID

3967323; -.

GenomeReviews

CP000282_GR; Sde_0084.

KEGG

sde:Sde_0084; -.

NMPDR

fig|203122.1.peg.2635; -.

Phylogenomic databases

HOGENOM

Q21PN1; -.

Genome annotation databases

CMR

Q21PN1; Sde_0084.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

4Fe-4S; Complete proteome; Electron transport; Iron; Iron-sulfur; Metal-binding; Molybdenum; Nitrate assimilation; Oxidoreductase; Periplasm; Signal; Transport.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`SIGNAL`	`1 29`	`29`	`Tat-type signal (Potential).`
`CHAIN`	`30 831`	`802`	`Periplasmic nitrate reductase.`	`PRO_0000256076`
`METAL`	`48 48`		`Iron-sulfur (4Fe-4S) (By similarity).`
`METAL`	`51 51`		`Iron-sulfur (4Fe-4S) (By similarity).`
`METAL`	`55 55`		`Iron-sulfur (4Fe-4S) (By similarity).`
`METAL`	`83 83`		`Iron-sulfur (4Fe-4S) (By similarity).`

Sequence information

Length: 831 AA [This is the length of the unprocessed precursor]

Molecular weight: 93164 Da [This is the MW of the unprocessed precursor]

CRC64: 8B6EA1C70B7AF770 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MTVTRRDFVR HQALATAAAA AGVAVPAAAT NIVTTAADNK LVWSKAPCRF CGTGCSVNVA 

        70         80         90        100        110        120 
TKEGRVVATH GDIKSPVNRG LNCVKGYFLS KVMYGEDRLT QPLLRKKNGV YAKDGEFEPV 

       130        140        150        160        170        180 
TWEEAFTIMA EKFKKTLKEK GPEGIGMFGS GQWTVWEGYA ASKLMKAGFR SNNIDPNARH 

       190        200        210        220        230        240 
CMASAVGGFM RTFGIDEPMG CYDDFEHADA FVLWGSNMAE MHPILWTRIA DRRLSYPHVQ 

       250        260        270        280        290        300 
VAVMSTYEHR SFDLADLGVV FTPQSDLAIA NFIANYIIQN KKVNWDFVKK HTNFRVGTTD 

       310        320        330        340        350        360 
IGYGLRPEHP LQKAAKNADS AGASEPIDFE TYAKFVADYT VEKASEISGV SEDKLIKLAK 

       370        380        390        400        410        420 
LYADPNIKVM SLWTMGVNQH TRGVWMNNLI YNIHLLTGKI SEPGNSPFSL TGQPSACGTA 

       430        440        450        460        470        480 
REVGTFSHRL PADMVVTNPK HRAYAEKIWK LPEGSIPEKV GAHAVLQSRK LKDGEINAYW 

       490        500        510        520        530        540 
VQVNNNVQAG PNINEEVLPG YRNPQNFIVV SDAYPTVTAQ AADLILPSAM WVEKEGAYGN 

       550        560        570        580        590        600 
AERRTQFWHQ LVNAPGDARS DLWQLVEFSK YFKVEEVWPA DLIAKMPEAK GKTLFDILYK 

       610        620        630        640        650        660 
NGKVNKFPLA QVSKDYENKE ADAFGFYIQK GLFEEYAEFG RGHGHDLADF DRYHEERGLR 

       670        680        690        700        710        720 
WPVVNGEETL WRYREGSDPY VEKGKGVQFY GKPDGKAIIF ALPYEPPAES PNKEYPFWLC 

       730        740        750        760        770        780 
TGRVIEHWHS GSMTQRVPEL YKAFPDAVCF MHPEDARSAG LRRGDKVKLQ SIRGHIITRI 

       790        800        810        820        830 
ETRGRNKPPK GLVFVPWFDA RQLINKVTLD ATDPISKQTD FKKCAVKVER V

Q21PN1 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools