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UniProtKB/Swiss-Prot entry Q03220

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name CTL1_YEAST
Primary accession number Q03220
Secondary accession numbers None
Integrated into Swiss-Prot on November 1, 1997
Sequence was last modified on November 1, 1997 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 57)
Name and origin of the protein
Protein name Polynucleotide 5'-triphosphatase
Synonyms EC 3.1.3.33
mRNA 5'-triphosphatase
TPase
Gene name
Name: CTL1
OrderedLocusNames: YMR180C
ORFNames: YM8010.10C
From
Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 204511 / S288c / AB972;
PubMed=9169872 [NCBI, ExPASy, EBI, Israel, Japan]
Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T., Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K., Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P., Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
"The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
Nature 387:90-93(1997).
[2]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC 204508 / S288c;
DOI=10.1101/gr.6037607; PubMed=17322287 [NCBI, ExPASy, EBI, Israel, Japan]
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J., Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.;
"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae.";
Genome Res. 17:536-543(2007).
[3]
 CHARACTERIZATION.
DOI=10.1093/nar/27.10.2181; PubMed=10219091 [NCBI, ExPASy, EBI, Israel, Japan]
Rodriguez C.R., Takagi T., Cho E.J., Buratowski S.;
"A Saccharomyces cerevisiae RNA 5'-triphosphatase related to mRNA capping enzyme.";
Nucleic Acids Res. 27:2181-2188(1999).
Comments
  • FUNCTION: Probably involved in an RNA processing event other than mRNA capping. Releases gamma-phosphate from the 5'-end of RNA to produce a diphosphate terminus.
  • CATALYTIC ACTIVITY: A 5'-phosphopolynucleotide + H2O = a polynucleotide + phosphate.
  • COFACTOR: Magnesium and/or manganese. Specific for polynucleotide RNA in the presence of magnesium, but becomes specific for nucleotide triphosphates in the presence of manganese.
  • SUBCELLULAR LOCATION: Cytoplasm. Nucleus.
  • SIMILARITY: Belongs to the fungal TPase family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
Z49808; CAA89913.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY693065; AAT93084.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S55127; S55127.
RefSeq NP_013905.1; -.
3D structure databases
HSSP O13297; 1D8I. [HSSP ENTRY / PDB]
ModBase Q03220.
Protein-protein interaction databases
DIP DIP:1776N; -.
IntAct Q03220; -.
Organism-specific databases
CYGD YMR180c; -.
SGD S000004792; CTL1.
Yeast-GFP YMR180C.
Gene expression databases
GermOnline YMR180C; Saccharomyces cerevisiae.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from electronic annotation from UniProtKB-KW).
GO:0005634; Cellular component: nucleus (inferred from electronic annotation from InterPro).
GO:0004651; Molecular function: polynucleotide 5'-phosphatase activity (inferred from electronic annotation from InterPro).
GO:0006370; Biological process: mRNA capping (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR004206; mRNA_triPase.
Graphical view of domain structure.
Pfam PF02940; mRNA_triPase; 1.
Pfam graphical view of domain structure.
ProtoNet Q03220.
Genome annotation databases
Ensembl YMR180C; Saccharomyces cerevisiae. [Contig view]
GeneID 855218; -.
GenomeReviews Z71257_GR; YMR180C.
KEGG sce:YMR180C; -.
NMPDR fig|4932.3.peg.4956; -.
Phylogenomic databases
HOGENOM Q03220; -.
Other
LinkHub Q03220; -.
NextBio 978732; -.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Cytoplasm; Hydrolase; mRNA processing; Nucleus.
Features
SEVIEWER logo Feature table viewer
KeyFrom To Length Description FTId
CHAIN   1   320  320     Polynucleotide 5'-triphosphatase. PRO_0000210120
Sequence information
Length: 320 AA [This is the length of the unprocessed precursor] Molecular weight: 36974 Da [This is the MW of the unprocessed precursor] CRC64: 82EFBDF3E100B57B [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MSDQPETPSN SRNSHENVGA KKADANVASK FRSLHISETT KPLTSTRALY KTTRNNSRGA 

        70         80         90        100        110        120 
TEFHKHVCKL AWKYLACIDK SSISHIEIEM KFGVITDKRT HRRMTPHNKP FIVQNRNGRL 

       130        140        150        160        170        180 
VSNVPEQMFS SFQELLRSKS ENPSKCAPRV VKQVQKYTKD SIYNCNNASK VGKLTSWRCS 

       190        200        210        220        230        240 
EDLRNKELKL TYIKKVRVKD FLIRYPQSSL DAKISISLEV PEYETSAAFR NGFILQRTKS 

       250        260        270        280        290        300 
RSTYTFNDKM PLHLDLTKVT TTRRNSHQYT SHEVEVEMDP IFKETISAND REKFNEYMCS 

       310        320 
FLNASDLIRK AAERDNMLTT
Q03220 in FASTA format

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