[1]	NUCLEOTIDE SEQUENCE. TISSUE=Spinal cord; DOI=10.1016/0169-328X(90)90092-R; PubMed=2160042 [NCBI, ExPASy, EBI, Israel, Japan] Ishii K., Oda Y., Ichikawa T., Deguchi T.; "Complementary DNAs for choline acetyltransferase from spinal cords of rat and mouse: nucleotide sequences, expression in mammalian cells, and in situ hybridization."; Brain Res. Mol. Brain Res. 7:151-159(1990).
[2]	NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] OF 1-219. TISSUE=Spinal cord; PubMed=1400357 [NCBI, ExPASy, EBI, Israel, Japan] Misawa H., Ishii K., Deguchi T.; "Gene expression of mouse choline acetyltransferase. Alternative splicing and identification of a highly active promoter region."; J. Biol. Chem. 267:20392-20399(1992).

Comments

FUNCTION: Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
CATALYTIC ACTIVITY: Acetyl-CoA + choline = CoA + O-acetylcholine.
SIMILARITY: Belongs to the carnitine/choline acetyltransferase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

D12487; BAA02056.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
D12486; BAA02055.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
D12488; BAA20976.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
D12489; BAA20977.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
D12490; BAA02057.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
D12491; BAA20978.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
D12492; BAA20979.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
D12493; BAA20980.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

IPI00224045; -.

B43777; B43777.

3D structure databases

HSSP

P47934; 1NDF. [HSSP ENTRY / PDB]

Q03059; 24-616.

PTM databases

Q03059; -.

Enzyme and pathway databases

BRENDA

2.3.1.6; 244.

Organism-specific databases

MGI

MGI:88392; Chat.

Gene expression databases

Q03059; -.

Q03059; -.

MM_CHAT; -.

ENSMUSG00000021919; Mus musculus.

Ontologies

GO:0043025;	Cellular component: cell soma (inferred from direct assay from MGI).
GO:0004102;	Molecular function: choline O-acetyltransferase activity (inferred from mutant phenotype from MGI).
GO:0007628;	Biological process: adult walking behavior (inferred from mutant phenotype from MGI).
GO:0016358;	Biological process: dendrite development (inferred from mutant phenotype from MGI).
GO:0007529;	Biological process: establishment of synaptic specificity at neuromuscular junction (inferred from mutant phenotype from MGI).
GO:0007274;	Biological process: neuromuscular synaptic transmission (inferred from mutant phenotype from MGI).
GO:0042136;	Biological process: neurotransmitter biosynthetic process (inferred from electronic annotation from UniProtKB-KW).
GO:0007622;	Biological process: rhythmic behavior (inferred from mutant phenotype from MGI).
GO:0043179;	Biological process: rhythmic excitation (inferred from mutant phenotype from MGI).
QuickGo view.

Family and domain databases

InterPro

IPR000542; Carn_acyl_trans.
Graphical view of domain structure.

PANTHER

PTHR22589; Carn_acyl_trans; 1.

Pfam

PF00755; Carn_acyltransf; 1.
Pfam graphical view of domain structure.

PROSITE

PS00439; ACYLTRANSF_C_1; 1.
PS00440; ACYLTRANSF_C_2; 1.

Genome annotation databases

Ensembl

ENSMUSG00000021919; Mus musculus. [Contig view]

Phylogenomic databases

HOVERGEN

Q03059; -.

Other

Chat; Mus musculus.

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Acyltransferase; Neurotransmitter biosynthesis; Transferase.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`INIT_MET`	`1 1`		`Removed (By similarity).`
`CHAIN`	`2 641`	`640`	`Choline O-acetyltransferase.`	`PRO_0000210155`
`REGION`	`413 425`	`13`	`Coenzyme A binding (By similarity).`
`ACT_SITE`	`335 335`		`Proton acceptor (By similarity).`
`BINDING`	`451 451`		`Coenzyme A (By similarity).`
`BINDING`	`552 552`		`Coenzyme A (By similarity).`

Sequence information

Length: 641 AA [This is the length of the unprocessed precursor]

Molecular weight: 71853 Da [This is the MW of the unprocessed precursor]

CRC64: 9BE0010779C8AE6D [This is a checksum on the sequence]

        10         20         30         40         50         60 
MPILEKVPPK MPVQASSCEE VLDLPKLPVP PLQQTLATYL QCMQHLVPEE QFRKSQAIVK 

        70         80         90        100        110        120 
RFGAPGGLGE TLQEKLLERQ EKTANWVSEY WLNDMYLNNR LALPVNSSPA VIFARQHFQD 

       130        140        150        160        170        180 
TNDQLRFAAS LISGVLSYKA LLDSQSIPTD WAKGQLSGQP LCMKQYYRLF SSYRLPGHTQ 

       190        200        210        220        230        240 
DTLVAQKSSI MPEPEHVIVA CCNQFFVLDV VINFRRLSEG DLFTQLRKIV KMASNEDERL 

       250        260        270        280        290        300 
PPIGLLTSDG RSEWAKARTV LLKDSTNRDS LDMIERCICL VCLDGPGTGD LSDTHRALQL 

       310        320        330        340        350        360 
LHGGGCSLNG ANRWYDKSLQ FVVGRDGTCG VVCEHSPFDG IVLVQCTEHL LKHMMTGNKK 

       370        380        390        400        410        420 
LVRVDSVSEL PAPRRLRWKC SPETQGHLAS SAEKLQRIVK NLDFIVYKFD NYGKTFIKKQ 

       430        440        450        460        470        480 
KCSPDGFIQV ALQLAYYRLY QRLVPTYESA SIRRFQEGRV DNIRSATPEA LAFVQAMTDH 

       490        500        510        520        530        540 
KAAVLASEKL QLLQRAIQAQ TEYTVMAITG MAIDNHLLAL RELARDLCKE PPEMFMDETY 

       550        560        570        580        590        600 
LMSNRFILST SQVPTTMEMF CCYGPVVPNG YGACYNPHAE AITFCISSFH GCKETSSVEF 

       610        620        630        640 
AEAVGASLVD MRDLCSSRQP ADSKPPTAKE RARGPSQAKQ S

Q03059 in FASTA format

View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools