PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, BIOPHYSICOCHEMICAL PROPERTIES, AND SUBUNIT.
TISSUE=Spore;
DOI=10.1007/s00284-001-0101-x; PubMed=11910495 [NCBI, ExPASy, EBI, Israel, Japan]
Aguilar M., Montalbini P., Pineda M.;
"Urate oxidase from the rust Puccinia recondita is a heterotetramer with two different-sized monomers.";
Curr. Microbiol. 44:257-261(2002).

Comments

FUNCTION: Catalyzes the oxidation of uric acid to 5-hydroxyisourate, which spontaneously decomposes to form allantoin.
CATALYTIC ACTIVITY: Urate + O₂ + H₂O = 5-hydroxyisourate + H₂O₂.
ENZYME REGULATION: Inhibited by two structural analogs of urate, oxonate and xanthine.
BIOPHYSICOCHEMICAL PROPERTIES:

Kinetic parameters: K_M=35 µM for Urate;
pH dependence: Optimum pH is 9.0;
PATHWAY: Purine metabolism; uric acid degradation; (S)-allantoin from uric acid: step 1/3 .
SUBUNIT: Heterotetramer.
SIMILARITY: Belongs to the uricase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

3D structure databases

ModBase

P82858.

Ontologies

GO:0004846;	Molecular function: urate oxidase activity (inferred from direct assay from UniProtKB).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
GO:0006144;	Biological process: purine base metabolic process (inferred from direct assay from UniProtKB).
QuickGo view.

Family and domain databases

InterPro

IPR002042; Uricase.
Graphical view of domain structure.

PROSITE

PS00366; URICASE; PARTIAL.

ProtoNet

P82858.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Direct protein sequencing; Oxidoreductase; Purine metabolism.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 >8`	`>8`	`Uricase.`	`PRO_0000262767`
`NON_TER`	`8 8`

Sequence information

Length: 8 AA [This is the length of the partial sequence of the unprocessed precursor]

Molecular weight: 777 Da [This is the MW of the partial sequence of the unprocessed precursor]

CRC64: 98C1ADD735B9D76D [This is a checksum on the sequence]

APFSLATA

P82858 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools