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UniProtKB/Swiss-Prot entry P62328


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name TYB4_HUMAN
Primary accession number P62328
Secondary accession numbers P01253 P01254 Q546P5 Q63576 Q9UE55
Integrated into Swiss-Prot on July 21, 1986
Sequence was last modified on January 23, 2007 (Sequence version 2)
Annotations were last modified on    June 16, 2009 (Entry version 53)
Name and origin of the protein
Protein name Thymosin beta-4
Synonyms T beta-4
Fx
Contains Hematopoietic system regulatory peptide
     (Seraspenide)
Gene name
Name: TMSB4X
Synonyms: TB4X, THYB4, TMSB4
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA], INDUCTION, AND TISSUE SPECIFICITY.
TISSUE=Peripheral blood leukocyte;
PubMed=3500230 [NCBI, ExPASy, EBI, Israel, Japan]
Gondo H., Kudo J., White J.W., Barr C., Selvanayagam P., Saunders G.F.;
"Differential expression of the human thymosin-beta 4 gene in lymphocytes, macrophages, and granulocytes.";
J. Immunol. 139:3840-3848(1987).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1007/s11010-005-7642-4; PubMed=16010977 [NCBI, ExPASy, EBI, Israel, Japan]
Yang S.P., Lee H.J., Su Y.;
"Molecular cloning and structural characterization of the functional human thymosin beta4 gene.";
Mol. Cell. Biochem. 272:97-105(2005).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.;
"Cloning of human full-length CDSs in BD Creator(TM) system donor vector.";
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
[4]
PROTEIN SEQUENCE OF 2-44, AND INTERACTION WITH ACTIN.
PubMed=1999398 [NCBI, ExPASy, EBI, Israel, Japan]
Safer D., Elzinga M., Nachmias V.T.;
"Thymosin beta 4 and Fx, an actin-sequestering peptide, are indistinguishable.";
J. Biol. Chem. 266:4029-4032(1991).
[5]
NUCLEOTIDE SEQUENCE [MRNA] OF 8-44, AND INDUCTION.
DOI=10.1016/0092-8674(84)90270-8; PubMed=6548414 [NCBI, ExPASy, EBI, Israel, Japan]
Friedman R.L., Manly S.P., McMahon M., Kerr I.M., Stark G.R.;
"Transcriptional and posttranscriptional regulation of interferon-induced gene expression in human cells.";
Cell 38:745-755(1984).
[6]
STRUCTURE BY NMR OF WILD-TYPE AND OF MUTANTS LYS-12; SER-16 AND LEU-18 IN COMPLEX WITH ACTIN.
DOI=10.1046/j.1432-1327.2000.01380.x; PubMed=10848969 [NCBI, ExPASy, EBI, Israel, Japan]
Simenel C., Van Troys M., Vandekerckhove J., Ampe C., Delepierre M.;
"Structural requirements for thymosin beta4 in its contact with actin. An NMR-analysis of thymosin beta4 mutants in solution and correlation with their biological activity.";
Eur. J. Biochem. 267:3530-3538(2000).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M17733; AAA36745.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AJ295158; CAC43317.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BT007090; AAP35753.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X02493; CAA26323.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
IPI IPI00220828; -.
PIR I56000; A38682.
RefSeq NP_066932.1; -.
UniGene Hs.522584
3D structure databases
PDB
1UY5; Model; -; T=2-44.[ExPASy / RCSB / EBI]
PDBsum 1UY5; -.
DisProt DP00357; -.
ModBase P62328.
Protein-protein interaction databases
IntAct P62328; 8.
PTM databases
PhosphoSite P62328; -.
Enzyme and pathway databases
Reactome REACT_604; Hemostasis.
2D gel databases
DOSAC-COBS-2DPAGE P62328; -.
Organism-specific databases
GeneCards GC0XP012903; -.
H-InvDB HIX0016658; -.
HGNC HGNC:11881; TMSB4X.
GenAtlas TMSB4X.
MIM 300159; gene. [NCBI / EBI]
PharmGKB PA36581; -.
Gene expression databases
ArrayExpress P62328; -.
Bgee P62328; -.
CleanEx HS_TMSB4X; -.
GermOnline ENSG00000205542; Homo sapiens.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from electronic annotation from UniProtKB-SubCell).
GO:0005856; Cellular component: cytoskeleton (inferred from electronic annotation from UniProtKB-SubCell).
GO:0003779; Molecular function: actin binding (inferred from electronic annotation from UniProtKB-KW).
GO:0030036; Biological process: actin cytoskeleton organization (inferred from electronic annotation from InterPro).
GO:0042989; Biological process: sequestering of actin monomers (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR001152; Thymosin_b4.
IPR016323; Thymosin_b4_chordata.
Graphical view of domain structure.
Gene3D G3DSA:1.20.5.520; Thymosin_b4; 1.
Pfam PF01290; Thymosin; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF001828; Thymosin_beta; 1.
ProDom PD005116; Thymosin_b4; 1.
[Domain structure / List of seq. sharing at least 1 domain]
SMART SM00152; THY; 1.
SMART graphical view of domain structure.
PROSITE PS00500; THYMOSIN_B4; 1.
Proteomic databases
PRIDE P62328; -.
Genome annotation databases
Ensembl ENSG00000205542; Homo sapiens. [Contig view]
GeneID 7114; -.
KEGG hsa:7114; -.
Phylogenomic databases
HOGENOM P62328; -.
HOVERGEN P62328; -.
Other
NextBio 27851; -.
SOURCE TMSB4X; Homo sapiens.
ProtoNet P62328.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Acetylation; Actin-binding; Cytoplasm; Cytoskeleton; Direct protein sequencing; Phosphoprotein.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom  To Length Description FTId
INIT_MET   1    1        Removed. 
PEPTIDE   2   44  43     Thymosin beta-4. PRO_0000045920
PEPTIDE   2    5  4     Hematopoietic system regulatory peptide (By similarity). PRO_0000034295
MOD_RES   2    2        N-acetylserine. 
MOD_RES   23   23        Phosphothreonine (By similarity). 
MUTAGEN   12   12        K->P: Very weak actin binding; no inhibition of actin polymerization. 
MUTAGEN   16   16        S->A: Binds actin 2.5-fold less than wild-type; little change in inhibition of actin polymerization. 
MUTAGEN   16   16        S->AS: Very weak actin binding; no inhibition of actin polymerization. 
MUTAGEN   18   18        L->A,P: Very weak actin binding; no inhibition of actin polymerization. 
Sequence information
Length: 44 AA [This is the length of the unprocessed precursor] Molecular weight: 5053 Da [This is the MW of the unprocessed precursor] CRC64: 440C6158482DAAD0 [This is a checksum on the sequence]
        10         20         30         40 
MSDKPDMAEI EKFDKSKLKK TETQEKNPLP SKETIEQEKQ AGES 

P62328 in FASTA format

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