ID   AF2_GRARO               Reviewed;          31 AA.
AC   P61409;
DT   24-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2004, sequence version 1.
DT   22-JUL-2008, entry version 17.
DE   RecName: Full=Mechanotoxin-alpha;
DE   AltName: Full=Peptide GsAF II;
OS   Grammostola rosea (Chilean rose tarantula) (Grammostola spatulata).
OC   Eukaryota; Metazoa; Arthropoda; Chelicerata; Arachnida; Araneae;
OC   Mygalomorphae; Theraphosidae; Grammostola.
OX   NCBI_TaxID=432528;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, AND MASS SPECTROMETRY.
RC   TISSUE=Venom;
RA   Lampe R.A.;
RT   "Analgesic peptides from venom of Grammostola spatulata and use
RT   thereof.";
RL   Patent number US5877026, 02-MAR-1999.
RN   [2]
RP   PROTEIN SEQUENCE, FUNCTION, AND MASS SPECTROMETRY.
RC   TISSUE=Venom;
RA   Lampe R.A.;
RT   "Antiarrhythmic peptide from venom of spider Grammostola spatulata.";
RL   Patent number US5968838, 19-OCT-1999.
CC   -!- FUNCTION: Blocks mechanosensitive ion channels and the hypotonic
CC       cell swelling induced calcium increase associated with the
CC       activation of such channels. It can thus be useful in treating
CC       cardiac ventricular disturbances. Also induces analgesia in
CC       mammals. This analgesia is probably mediated by a non-opioid
CC       receptor related mechanism.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC   -!- DOMAIN: The presence of a 'disulfide through disulfide knot'
CC       structurally defines this protein as a knottin (By similarity).
CC   -!- MASS SPECTROMETRY: Mass=3979.9; Method=Electrospray; Range=1-31;
CC       Source=Ref.1, Ref.2;
CC   -!- SIMILARITY: Belongs to the phrixotoxin family.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Knottin; Secreted; Toxin.
FT   PEPTIDE       1     31       Mechanotoxin-alpha.
FT                                /FTId=PRO_0000045016.
FT   DISULFID      2     16       By similarity.
FT   DISULFID      9     21       By similarity.
FT   DISULFID     15     25       By similarity.
SQ   SEQUENCE   31 AA;  3986 MW;  BF03EB84DE1AAC1B CRC64;
     YCQKWMWTCD EERKCCEGLV CRLWCKKKIE W
//