ID   GBRL2_HUMAN             Reviewed;         117 AA.
AC   P60520; O08765; Q9DCP8; Q9UQF7;
DT   01-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2004, sequence version 1.
DT   22-JUL-2008, entry version 42.
DE   RecName: Full=Gamma-aminobutyric acid receptor-associated protein-like 2;
DE   AltName: Full=GABA(A) receptor-associated protein-like 2;
DE   AltName: Full=Golgi-associated ATPase enhancer of 16 kDa;
DE            Short=GATE-16;
DE   AltName: Full=General protein transport factor p16;
DE   AltName: Full=Ganglioside expression factor 2;
DE            Short=GEF-2;
DE   AltName: Full=MAP1 light chain 3-related protein;
GN   Name=GABARAPL2; Synonyms=FLC3A, GEF2;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND INTERACTION WITH
RP   ULK1.
RC   TISSUE=Frontal cortex;
RX   MEDLINE=21066693; PubMed=11146101; DOI=10.1016/S0169-328X(00)00218-7;
RA   Okazaki N., Yan J., Yuasa S., Ueno T., Kominami E., Masuho Y.,
RA   Koga H., Muramatsu M.-A.;
RT   "Interaction of the Unc-51-like kinase and microtubule-associated
RT   protein light chain 3 related proteins in the brain: possible role of
RT   vesicular transport in axonal elongation.";
RL   Brain Res. Mol. Brain Res. 85:1-12(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RX   MEDLINE=21309076; PubMed=11414770; DOI=10.1006/geno.2001.6555;
RA   Xin Y., Yu L., Chen Z., Zheng L., Fu Q., Jiang J., Zhang P., Gong R.,
RA   Zhao S.;
RT   "Cloning, expression patterns, and chromosome localization of three
RT   human and two mouse homologues of GABA(A) receptor-associated
RT   protein.";
RL   Genomics 74:408-413(2001).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Storch S., Braulke T.;
RL   Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Pituitary;
RA   Song H., Peng Y., Yu Y., Fu G., Mao M., Zhang Q., Zhu H., Li G.,
RA   Luo M., Chen J., Hu R.;
RT   "Human GEF2 homolog gene.";
RL   Submitted (JUL-1998) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Brain, and Testis;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [6]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-10, AND MASS
RP   SPECTROMETRY.
RX   PubMed=17287340; DOI=10.1073/pnas.0611217104;
RA   Molina H., Horn D.M., Tang N., Mathivanan S., Pandey A.;
RT   "Global proteomic profiling of phosphopeptides using electron transfer
RT   dissociation tandem mass spectrometry.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:2199-2204(2007).
RN   [7]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-39, AND MASS
RP   SPECTROMETRY.
RX   PubMed=17525332; DOI=10.1126/science.1140321;
RA   Matsuoka S., Ballif B.A., Smogorzewska A., McDonald E.R. III,
RA   Hurov K.E., Luo J., Bakalarski C.E., Zhao Z., Solimini N.,
RA   Lerenthal Y., Shiloh Y., Gygi S.P., Elledge S.J.;
RT   "ATM and ATR substrate analysis reveals extensive protein networks
RT   responsive to DNA damage.";
RL   Science 316:1160-1166(2007).
CC   -!- FUNCTION: Involved in intra-Golgi traffic. Modulates intra-Golgi
CC       transport through coupling between NSF activity and SNAREs
CC       activation. It first stimulates the ATPase activity of NSF which
CC       in turn stimulates the association with GOSR1 (By similarity).
CC   -!- SUBUNIT: Monomer. Interacts with GABRG2, NSF, GOSR1 and beta-
CC       tubulin (By similarity). Interacts with ULK1.
CC   -!- INTERACTION:
CC       Q9NT62:ATG3; NbExp=1; IntAct=EBI-720116, EBI-988094;
CC       Q9Y4P1:ATG4B; NbExp=1; IntAct=EBI-720116, EBI-712014;
CC       Q04637:EIF4G1; NbExp=1; IntAct=EBI-720116, EBI-73711;
CC       P69891:HBG1; NbExp=1; IntAct=EBI-720116, EBI-1056789;
CC       Q8WZA9:IRGQ; NbExp=1; IntAct=EBI-720116, EBI-1055331;
CC       P42704:LRPPRC; NbExp=1; IntAct=EBI-720116, EBI-1050853;
CC       Q8TD19:NEK9; NbExp=1; IntAct=EBI-720116, EBI-1044009;
CC       P21796:VDAC1; NbExp=1; IntAct=EBI-720116, EBI-354158;
CC       P15311:VIL2; NbExp=1; IntAct=EBI-720116, EBI-1056902;
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus (By similarity).
CC   -!- TISSUE SPECIFICITY: Ubiquitous. Expressed at high levels in the
CC       brain, heart, prostate, ovary, spleen and skeletal muscle.
CC       Expressed at very low levels in lung, thymus and small intestine.
CC   -!- PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
CC   -!- SIMILARITY: Belongs to the MAP1 LC3 family.
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DR   EMBL; AB030710; BAB21548.1; -; mRNA.
DR   EMBL; AF087848; AAK20400.1; -; mRNA.
DR   EMBL; AJ010569; CAA09249.1; -; mRNA.
DR   EMBL; AF077046; AAD27779.1; -; mRNA.
DR   EMBL; BC005985; AAH05985.1; -; mRNA.
DR   EMBL; BC014594; AAH14594.1; -; mRNA.
DR   EMBL; BC029601; AAH29601.1; -; mRNA.
DR   RefSeq; NP_009216.1; -.
DR   UniGene; Hs.461379; -.
DR   SMR; P60520; 1-117.
DR   IntAct; P60520; -.
DR   PhosphoSite; P60520; -.
DR   Ensembl; ENSG00000034713; Homo sapiens.
DR   GeneID; 11345; -.
DR   KEGG; hsa:11345; -.
DR   H-InvDB; HIX0013248; -.
DR   HGNC; HGNC:13291; GABARAPL2.
DR   MIM; 607452; gene.
DR   PharmGKB; PA28482; -.
DR   HOGENOM; P60520; -.
DR   HOVERGEN; P60520; -.
DR   ArrayExpress; P60520; -.
DR   CleanEx; HS_GABARAPL2; -.
DR   GermOnline; ENSG00000034713; Homo sapiens.
DR   GO; GO:0000421; C:autophagic vacuole membrane; IDA:UniProtKB.
DR   GO; GO:0005829; C:cytosol; ISS:UniProtKB.
DR   GO; GO:0000139; C:Golgi membrane; ISS:UniProtKB.
DR   GO; GO:0005624; C:membrane fraction; IDA:UniProtKB.
DR   GO; GO:0051117; F:ATPase binding; ISS:UniProtKB.
DR   GO; GO:0048487; F:beta-tubulin binding; ISS:UniProtKB.
DR   GO; GO:0050811; F:GABA receptor binding; NAS:UniProtKB.
DR   GO; GO:0008017; F:microtubule binding; NAS:UniProtKB.
DR   GO; GO:0000149; F:SNARE binding; ISS:UniProtKB.
DR   GO; GO:0006914; P:autophagy; NAS:UniProtKB.
DR   GO; GO:0006891; P:intra-Golgi vesicle-mediated transport; ISS:UniProtKB.
DR   GO; GO:0032781; P:positive regulation of ATPase activity; ISS:UniProtKB.
DR   InterPro; IPR004241; MAP1_LC3.
DR   PANTHER; PTHR10969; MAP1_LC3; 1.
DR   Pfam; PF02991; MAP1_LC3; 1.
PE   1: Evidence at protein level;
KW   Golgi apparatus; Phosphoprotein; Protein transport; Transport.
FT   CHAIN         1    117       Gamma-aminobutyric acid receptor-
FT                                associated protein-like 2.
FT                                /FTId=PRO_0000212373.
FT   REGION        1     22       Interaction with beta-tubulin (By
FT                                similarity).
FT   REGION       36     68       Interaction with GABRG2 (Potential).
FT   MOD_RES      10     10       Phosphoserine.
FT   MOD_RES      39     39       Phosphoserine.
FT   CONFLICT     29     29       V -> F (in Ref. 4; AAD27779).
SQ   SEQUENCE   117 AA;  13667 MW;  17ACB540FD5B975B CRC64;
     MKWMFKEDHS LEHRCVESAK IRAKYPDRVP VIVEKVSGSQ IVDIDKRKYL VPSDITVAQF
     MWIIRKRIQL PSEKAIFLFV DKTVPQSSLT MGQLYEKEKD EDGFLYVAYS GENTFGF
//