GABA(A) receptor-associated protein-like 2
Golgi-associated ATPase enhancer of 16 kDa
GATE-16
General protein transport factor p16
Ganglioside expression factor 2
GEF-2
MAP1 light chain 3-related protein

Gene name

	Name:	GABARAPL2
	Synonyms:	GEF2

From

Bos taurus (Bovine)

[TaxID: 9913]

Taxonomy

Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia; Pecora; Bovidae; Bovinae; Bos.

Protein existence

1: Evidence at protein level;

References

[1]	NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 24-47; 55-65; 69-74 AND 107-116, FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND INTERACTION WITH NSF AND GOSR1. TISSUE=Brain; DOI=10.1093/emboj/19.7.1494; PubMed=10747018 [NCBI, ExPASy, EBI, Israel, Japan] Sagiv Y., Legesse-Miller A., Porat A., Elazar Z.; "GATE-16, a membrane transport modulator, interacts with NSF and the Golgi v-SNARE GOS-28."; EMBO J. 19:1494-1504(2000).
[2]	NUCLEOTIDE SEQUENCE [MRNA]. TISSUE=Kidney; DOI=10.1128/JVI.76.24.13069-13076.2002; PubMed=12438634 [NCBI, ExPASy, EBI, Israel, Japan] Becher P., Thiel H.-J., Collins M., Brownlie J., Orlich M.; "Cellular sequences in pestivirus genomes encoding gamma-aminobutyric acid (A) receptor-associated protein and Golgi-associated ATPase enhancer of 16 kilodaltons."; J. Virol. 76:13069-13076(2002).
[3]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. DOI=10.1186/1471-2164-6-166; PubMed=16305752 [NCBI, ExPASy, EBI, Israel, Japan] Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L., Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.; "Characterization of 954 bovine full-CDS cDNA sequences."; BMC Genomics 6:166-166(2005).
[4]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. STRAIN=Hereford; TISSUE=Hypothalamus; NIH - Mammalian Gene Collection (MGC) project; Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
[5]	X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS), AND SUBUNIT. DOI=10.1074/jbc.C000307200; PubMed=10856287 [NCBI, ExPASy, EBI, Israel, Japan] Paz Y., Elazar Z., Fass D.; "Structure of GATE-16, membrane transport modulator and mammalian ortholog of autophagocytosis factor Aut7p."; J. Biol. Chem. 275:25445-25450(2000).

Comments

FUNCTION: Involved in intra-Golgi traffic. Modulates intra-Golgi transport through coupling between NSF activity and SNAREs activation. It first stimulates the ATPase activity of NSF which in turn stimulates the association with GOSR1.
SUBUNIT: Interacts with GABRG2, ULK1 and beta-tubulin (By similarity). Monomer. Interacts with NSF and GOSR1.
SUBCELLULAR LOCATION: Golgi apparatus.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR (By similarity).
SIMILARITY: Belongs to the MAP1 LC3 family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AF020262; AAD20720.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AY117147; AAM77036.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BT020958; AAX08975.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BC102902; AAI02903.2; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_777100.1; -.

UniGene

Bt.41007

3D structure databases

PDB

1EO6; X-ray; 1.80 A; A/B=1-117.

[ExPASy / RCSB / EBI]

PDBsum

1EO6; -.

ModBase

P60519.

Ontologies

GO:0005829;	Cellular component: cytosol (inferred from direct assay from UniProtKB).
GO:0000139;	Cellular component: Golgi membrane (inferred from direct assay from UniProtKB).
GO:0051117;	Molecular function: ATPase binding (inferred from direct assay from UniProtKB).
GO:0000149;	Molecular function: SNARE binding (inferred from direct assay from UniProtKB).
GO:0006891;	Biological process: intra-Golgi vesicle-mediated transport (inferred from direct assay from UniProtKB).
GO:0032781;	Biological process: positive regulation of ATPase activity (inferred from direct assay from UniProtKB).
QuickGo view.

Family and domain databases

InterPro

IPR004241; MAP1_LC3.
Graphical view of domain structure.

PANTHER

PTHR10969; MAP1_LC3; 1.

Pfam

PF02991; MAP1_LC3; 1.
Pfam graphical view of domain structure.

BLOCKS

P60519.

Genome annotation databases

Ensembl

ENSBTAG00000006550; Bos taurus. [Contig view]

GeneID

282531; -.

KEGG

bta:282531; -.

Phylogenomic databases

HOVERGEN

P60519; -.

Other

P60519; -.

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

3D-structure; Direct protein sequencing; Golgi apparatus; Phosphoprotein; Protein transport; Transport.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 117`	`117`	`Gamma-aminobutyric acid receptor-associated protein-like 2.`	`PRO_0000212372`
`REGION`	`1 22`	`22`	`Interaction with beta-tubulin (By similarity).`
`REGION`	`36 68`	`33`	`Interaction with GABRG2 (Potential).`
`MOD_RES`	`10 10`		`Phosphoserine (By similarity).`
`MOD_RES`	`39 39`		`Phosphoserine (By similarity).`
`HELIX`	`4 8`	`5`
`HELIX`	`11 24`	`14`
`STRAND`	`28 35`	`8`
`STRAND`	`48 52`	`5`
`HELIX`	`57 68`	`12`
`STRAND`	`77 80`	`4`
`HELIX`	`91 98`	`8`
`STRAND`	`105 110`	`6`

Sequence information

Length: 117 AA [This is the length of the unprocessed precursor]

Molecular weight: 13667 Da [This is the MW of the unprocessed precursor]

CRC64: 17ACB540FD5B975B [This is a checksum on the sequence]

        10         20         30         40         50         60 
MKWMFKEDHS LEHRCVESAK IRAKYPDRVP VIVEKVSGSQ IVDIDKRKYL VPSDITVAQF 

        70         80         90        100        110 
MWIIRKRIQL PSEKAIFLFV DKTVPQSSLT MGQLYEKEKD EDGFLYVAYS GENTFGF

P60519 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools