ID   GBRAP_RAT               Reviewed;         117 AA.
AC   P60517; Q9QUI7;
DT   01-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2004, sequence version 1.
DT   22-JUL-2008, entry version 46.
DE   RecName: Full=Gamma-aminobutyric acid receptor-associated protein;
DE   AltName: Full=GABA(A) receptor-associated protein;
GN   Name=Gabarap;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, AND INTERACTION WITH
RP   BETA-TUBULIN.
RX   PubMed=10899939;
RA   Wang H., Olsen R.W.;
RT   "Binding of the GABA(A) receptor-associated protein (GABARAP) to
RT   microtubules and microfilaments suggests involvement of the
RT   cytoskeleton in GABARAPGABA(A) receptor interaction.";
RL   J. Neurochem. 75:644-655(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Pituitary;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [3]
RP   INTERACTION WITH GPHN.
RX   PubMed=10900017; DOI=10.1073/pnas.97.15.8594;
RA   Kneussel M., Haverkamp S., Fuhrmann J.C., Wang H., Waessle H.,
RA   Olsen R.W., Betz H.;
RT   "The gamma-aminobutyric acid type A receptor (GABAAR)-associated
RT   protein GABARAP interacts with gephyrin but is not involved in
RT   receptor anchoring at the synapse.";
RL   Proc. Natl. Acad. Sci. U.S.A. 97:8594-8599(2000).
RN   [4]
RP   FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND INTERACTION
RP   WITH NSF.
RX   PubMed=11461150; DOI=10.1006/mcne.2001.1005;
RA   Kittler J.T., Rostaing P., Schiavo G., Fritschy J.-M., Olsen R.,
RA   Triller A., Moss S.J.;
RT   "The subcellular distribution of GABARAP and its ability to interact
RT   with NSF suggest a role for this protein in the intracellular
RT   transport of GABA(A) receptors.";
RL   Mol. Cell. Neurosci. 18:13-25(2001).
RN   [5]
RP   X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS).
RX   PubMed=11818336; DOI=10.1093/embo-reports/kvf026;
RA   Bavro V.N., Sola M., Bracher A., Kneussel M., Betz H., Weissenhorn W.;
RT   "Crystal structure of the GABA(A)-receptor-associated protein,
RT   GABARAP.";
RL   EMBO Rep. 3:183-189(2002).
CC   -!- FUNCTION: May play a role in intracellular transport of GABA(A)
CC       receptors and its interaction with the cytoskeleton.
CC   -!- SUBUNIT: Interacts with GABRG2, TUBA1 and ULK1 (By similarity).
CC       Interacts with beta-tubulin, GPHN and NSF.
CC   -!- INTERACTION:
CC       Q9QUL6:Nsf; NbExp=1; IntAct=EBI-925812, EBI-925794;
CC   -!- SUBCELLULAR LOCATION: Intracytoplasmic membrane. Cytoplasm,
CC       cytoskeleton. Golgi apparatus membrane. Note=Largely associated
CC       with intracellular membrane structures including the Golgi
CC       apparatus and post-synaptic cisternae. Colocalizes with
CC       microtubules.
CC   -!- TISSUE SPECIFICITY: Can be found in both somatodendritic and
CC       axonal compartment of neurons.
CC   -!- SIMILARITY: Belongs to the MAP1 LC3 family.
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DR   EMBL; AF161588; AAD47643.1; -; mRNA.
DR   EMBL; BC058441; AAH58441.1; -; mRNA.
DR   RefSeq; NP_742033.1; -.
DR   UniGene; Rn.8411; -.
DR   PDB; 1KJT; X-ray; 2.00 A; A=1-117.
DR   PDBsum; 1KJT; -.
DR   IntAct; P60517; -.
DR   Ensembl; ENSRNOG00000017417; Rattus norvegicus.
DR   GeneID; 58974; -.
DR   KEGG; rno:58974; -.
DR   RGD; 61911; Gabarap.
DR   HOVERGEN; P60517; -.
DR   LinkHub; P60517; -.
DR   ArrayExpress; P60517; -.
DR   GermOnline; ENSRNOG00000017417; Rattus norvegicus.
DR   GO; GO:0005515; F:protein binding; IPI:IntAct.
DR   InterPro; IPR004241; MAP1_LC3.
DR   PANTHER; PTHR10969; MAP1_LC3; 1.
DR   Pfam; PF02991; MAP1_LC3; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Cytoplasm; Cytoskeleton; Golgi apparatus; Membrane;
KW   Microtubule; Protein transport; Transport.
FT   CHAIN         1    117       Gamma-aminobutyric acid receptor-
FT                                associated protein.
FT                                /FTId=PRO_0000212366.
FT   REGION        1     22       Interaction with beta-tubulin.
FT   REGION       36    117       Interaction with GPHN.
FT   REGION       36     68       Interaction with GABRG2 (Potential).
FT   HELIX         4      8
FT   HELIX        11     24
FT   STRAND       28     35
FT   STRAND       48     52
FT   HELIX        57     68
FT   STRAND       77     82
FT   HELIX        91     98
FT   STRAND      105    112
SQ   SEQUENCE   117 AA;  13918 MW;  BC0B84B8A51C1E32 CRC64;
     MKFVYKEEHP FEKRRSEGEK IRKKYPDRVP VIVEKAPKAR IGDLDKKKYL VPSDLTVGQF
     YFLIRKRIHL RAEDALFFFV NNVIPPTSAT MGQLYQEHHE EDFFLYIAYS DESVYGL
//