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UniProtKB/Swiss-Prot entry P54756


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name EPHA5_HUMAN
Primary accession number P54756
Secondary accession numbers None
Integrated into Swiss-Prot on October 1, 1996
Sequence was last modified on September 2, 2008 (Sequence version 2)
Annotations were last modified on    November 4, 2008 (Entry version 91)
Name and origin of the protein
Protein name Ephrin type-A receptor 5 [Precursor]
Synonyms EC 2.7.10.1
Tyrosine-protein kinase receptor EHK-1
EPH homology kinase 1
Receptor protein-tyrosine kinase HEK7
Gene name
Name: EPHA5
Synonyms: EHK1, HEK7
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
TISSUE=Brain;
DOI=10.1016/S0169-328X(96)00268-9; PubMed=9191074 [NCBI, ExPASy, EBI, Israel, Japan]
Miescher G.C., Taylor V., Olivieri G., Mindermann T., Shrock E., Steck A.J.;
"Extensive splice variation and localization of the EHK-1 receptor tyrosine kinase in adult human brain and glial tumors.";
Brain Res. Mol. Brain Res. 46:17-24(1997).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1038/nature03466; PubMed=15815621 [NCBI, ExPASy, EBI, Israel, Japan]
Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H., Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.;
"Generation and annotation of the DNA sequences of human chromosomes 2 and 4.";
Nature 434:724-731(2005).
[3]
NUCLEOTIDE SEQUENCE [MRNA] OF 25-1037.
TISSUE=Brain;
PubMed=7898931 [NCBI, ExPASy, EBI, Israel, Japan]
Fox G.M., Holst P.L., Chute H.T., Lindberg R.A., Janssen A.M., Basu R., Welcher A.A.;
"cDNA cloning and tissue distribution of five human EPH-like receptor protein-tyrosine kinases.";
Oncogene 10:897-905(1995).
[4]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-833, AND MASS SPECTROMETRY.
DOI=10.1016/j.cell.2007.11.025; PubMed=18083107 [NCBI, ExPASy, EBI, Israel, Japan]
Rikova K., Guo A., Zeng Q., Possemato A., Yu J., Haack H., Nardone J., Lee K., Reeves C., Li Y., Hu Y., Tan Z., Stokes M., Sullivan L., Mitchell J., Wetzel R., Macneill J., Ren J.M., Yuan J., Bakalarski C.E., Villen J., Kornhauser J.M., Smith B., Li D., Zhou X., Gygi S.P., Gu T.-L., Polakiewicz R.D., Rush J., Comb M.J.;
"Global survey of phosphotyrosine signaling identifies oncogenic kinases in lung cancer.";
Cell 131:1190-1203(2007).
[5]
VARIANTS [LARGE SCALE ANALYSIS] THR-81; ALA-235; GLN-330; GLN-417; LYS-503; CYS-506; GLU-582; THR-672; THR-673; ILE-856; ARG-959 AND SER-1032.
DOI=10.1038/nature05610; PubMed=17344846 [NCBI, ExPASy, EBI, Israel, Japan]
Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G., Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.;
"Patterns of somatic mutation in human cancer genomes.";
Nature 446:153-158(2007).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X95425; CAA64700.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AC018683; -; NOT_ANNOTATED_CDS; Genomic_DNA.[EMBL / GenBank / DDBJ]
AC104137; -; NOT_ANNOTATED_CDS; Genomic_DNA.[EMBL / GenBank / DDBJ]
AC105923; -; NOT_ANNOTATED_CDS; Genomic_DNA.[EMBL / GenBank / DDBJ]
AC115223; -; NOT_ANNOTATED_CDS; Genomic_DNA.[EMBL / GenBank / DDBJ]
L36644; AAA74245.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_004430.3; -.
NP_872272.1; -.
UniGene Hs.654492
3D structure databases
PDB
2R2P; X-ray; 2.40 A; A=653-940.[ExPASy / RCSB / EBI]
PDBsum 2R2P; -.
SMR P54756; 655-953.
ModBase P54756.
PTM databases
PhosphoSite P54756; -.
Organism-specific databases
H-InvDB HIX0024608; -.
HGNC HGNC:3389; EPHA5.
GenAtlas EPHA5.
MIM 600004; gene. [NCBI / EBI]
PharmGKB PA27821; -.
GeneCards P54756.
Gene expression databases
ArrayExpress P54756; -.
CleanEx HS_EPHA5; -.
GermOnline ENSG00000145242; Homo sapiens.
Ontologies
GO
GO:0016021; Cellular component: integral to membrane (traceable author statement from UniProtKB).
GO:0005005; Molecular function: transmembrane-ephrin receptor activity (traceable author statement from UniProtKB).
GO:0006468; Biological process: protein amino acid phosphorylation (traceable author statement from UniProtKB).
QuickGo view.
Family and domain databases
InterPro IPR015215; BmKX.
IPR013032; EGF_like_reg_CS.
IPR001090; Eph_rcpt_lig_bd.
IPR008957; Fibronectin_typ-III-like_fold.
IPR003961; FN_III.
IPR003962; FnIII_subd.
IPR000719; Prot_kinase_core.
IPR017441; Protein_kinase_ATP_bd_CS.
IPR001660; SAM.
IPR013761; SAM_type.
IPR001245; Tyr_pkinase.
IPR008266; Tyr_pkinase_AS.
IPR016257; TyrPK_ephrin_receptor.
IPR001426; YKase_receptorV_CS.
Graphical view of domain structure.
Gene3D G3DSA:2.60.40.30; FN_III-like; 2.
G3DSA:1.10.150.50; SAM_type; 1.
Pfam PF09132; BmKX; 1.
PF01404; Ephrin_lbd; 1.
PF00041; fn3; 2.
PF07714; Pkinase_Tyr; 1.
PF00536; SAM_1; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF000666; TyrPK_ephrin_receptor; 1.
PRINTS PR00014; FNTYPEIII.
PR00109; TYRKINASE.
ProDom PD001495; Ephrin_receptor; 1.
PD000001; Prot_kinase; 1.
[Domain structure / List of seq. sharing at least 1 domain]
SMART SM00615; EPH_lbd; 1.
SM00060; FN3; 2.
SM00454; SAM; 1.
SM00219; TyrKc; 1.
SMART graphical view of domain structure.
PROSITE PS01186; EGF_2; UNKNOWN_1.
PS50853; FN3; 2.
PS00107; PROTEIN_KINASE_ATP; 1.
PS50011; PROTEIN_KINASE_DOM; 1.
PS00109; PROTEIN_KINASE_TYR; 1.
PS00790; RECEPTOR_TYR_KIN_V_1; 1.
PS00791; RECEPTOR_TYR_KIN_V_2; 1.
PS50105; SAM_DOMAIN; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS P54756.
ProtoNet P54756.
Genome annotation databases
Ensembl ENSG00000145242; Homo sapiens. [Contig view]
GeneID 2044; -.
KEGG hsa:2044; -.
Phylogenomic databases
HOGENOM P54756; -.
HOVERGEN P54756; -.
Other
NextBio 8305; -.
SOURCE EPHA5; Homo sapiens.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Alternative splicing; ATP-binding; Glycoprotein; Kinase; Membrane; Nucleotide-binding; Phosphoprotein; Polymorphism; Receptor; Repeat; Signal; Transferase; Transmembrane; Tyrosine-protein kinase.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom    To Length Description FTId
SIGNAL   1     24  24     Potential. 
CHAIN   25   1037  1013     Ephrin type-A receptor 5. PRO_0000016812
TOPO_DOM   25    573  549     Extracellular (Potential). 
TRANSMEM   574    594  21     Potential. 
TOPO_DOM   595   1037  443     Cytoplasmic (Potential). 
DOMAIN   357    459  103     Fibronectin type-III 1. 
DOMAIN   469    559  91     Fibronectin type-III 2. 
DOMAIN   675    936  262     Protein kinase. 
DOMAIN   965   1029  65     SAM. 
NP_BIND   681    689  9     ATP (By similarity). 
MOTIF   1035   1037  3     PDZ-binding (Potential). 
COMPBIAS   220    354  135     Cys-rich. 
ACT_SITE   800    800        Proton acceptor (By similarity). 
BINDING   707    707        ATP (By similarity). 
MOD_RES   650    650        Phosphotyrosine; by autocatalysis (By similarity). 
MOD_RES   656    656        Phosphotyrosine; by autocatalysis (By similarity). 
MOD_RES   833    833        Phosphotyrosine; by autocatalysis (Potential). 
MOD_RES   982    982        Phosphotyrosine; by autocatalysis (By similarity). 
CARBOHYD   264    264        N-linked (GlcNAc...) (Potential). 
CARBOHYD   299    299        N-linked (GlcNAc...) (Potential). 
CARBOHYD   369    369        N-linked (GlcNAc...) (Potential). 
CARBOHYD   423    423        N-linked (GlcNAc...) (Potential). 
CARBOHYD   436    436        N-linked (GlcNAc...) (Potential). 
CARBOHYD   461    461        N-linked (GlcNAc...) (Potential). 
VAR_SEQ   597    619        SCCECGCGRASSLCAVAHPSLIW -> R (in isoform 2). VSP_002999
VARIANT   81     81  1     N -> T. VAR_042138 
VARIANT   235    235  1     S -> A. VAR_042139 
VARIANT   330    330  1     E -> Q. VAR_042140 
VARIANT   417    417  1     R -> Q (in a lung adenocarcinoma sample; somatic mutation). VAR_042141 
VARIANT   503    503  1     E -> K (in a lung large cell carcinoma sample; somatic mutation). VAR_042142 
VARIANT   506    506  1     Y -> C. VAR_045912 
VARIANT   582    582  1     G -> E (in a lung adenocarcinoma sample; somatic mutation). VAR_042143 
VARIANT   672    672  1     A -> T. VAR_042144 [3D]
VARIANT   673    673  1     S -> T. VAR_042145 [3D]
VARIANT   856    856  1     T -> I (in a lung squamous cell carcinoma sample; somatic mutation). VAR_042146 [3D]
VARIANT   959    959  1     H -> R. VAR_042147 [3D]
VARIANT   1032   1032  1     N -> S (in a lung large cell carcinoma sample; somatic mutation). VAR_042148 
CONFLICT   11     11        R -> H (in Ref. 1; CAA64700). 
CONFLICT   506    506        Y -> H (in Ref. 1; CAA64700). 
CONFLICT   616    616        S -> I (in Ref. 1; CAA64700). 
CONFLICT   642    642        S -> I (in Ref. 1; CAA64700). 
HELIX   663    665  3      
HELIX   672    674  3      
STRAND   675    683  9      
STRAND   685    694  10      
STRAND   702    708  7      
HELIX   715    728  14      
STRAND   739    743  5      
STRAND   745    748  4      
STRAND   750    754  5      
HELIX   761    766  6      
TURN   767    770  4      
HELIX   774    793  20      
HELIX   803    805  3      
STRAND   806    808  3      
STRAND   814    816  3      
HELIX   842    844  3      
HELIX   847    852  6      
HELIX   857    873  17      
TURN   878    881  4      
HELIX   884    893  10      
HELIX   905    914  10      
HELIX   919    921  3      
HELIX   925    936  12      
Sequence information
Length: 1037 AA [This is the length of the unprocessed precursor] Molecular weight: 114777 Da [This is the MW of the unprocessed precursor] CRC64: 22A8D1BF43902C7E [This is a checksum on the sequence]
        10         20         30         40         50         60 
MRGSGPRGAG RRRPPSGGGD TPITPASLAG CYSAPRRAPL WTCLLLCAAL RTLLASPSNE 

        70         80         90        100        110        120 
VNLLDSRTVM GDLGWIAFPK NGWEEIGEVD ENYAPIHTYQ VCKVMEQNQN NWLLTSWISN 

       130        140        150        160        170        180 
EGASRIFIEL KFTLRDCNSL PGGLGTCKET FNMYYFESDD QNGRNIKENQ YIKIDTIAAD 

       190        200        210        220        230        240 
ESFTELDLGD RVMKLNTEVR DVGPLSKKGF YLAFQDVGAC IALVSVRVYY KKCPSVVRHL 

       250        260        270        280        290        300 
AVFPDTITGA DSSQLLEVSG SCVNHSVTDE PPKMHCSAEG EWLVPIGKCM CKAGYEEKNG 

       310        320        330        340        350        360 
TCQVCRPGFF KASPHIQSCG KCPPHSYTHE EASTSCVCEK DYFRRESDPP TMACTRPPSA 

       370        380        390        400        410        420 
PRNAISNVNE TSVFLEWIPP ADTGGRKDVS YYIACKKCNS HAGVCEECGG HVRYLPRQSG 

       430        440        450        460        470        480 
LKNTSVMMVD LLAHTNYTFE IEAVNGVSDL SPGARQYVSV NVTTNQAAPS PVTNVKKGKI 

       490        500        510        520        530        540 
AKNSISLSWQ EPDRPNGIIL EYEIKYFEKD QETSYTIIKS KETTITAEGL KPASVYVFQI 

       550        560        570        580        590        600 
RARTAAGYGV FSRRFEFETT PVFAASSDQS QIPVIAVSVT VGVILLAVVI GVLLSGSCCE 

       610        620        630        640        650        660 
CGCGRASSLC AVAHPSLIWR CGYSKAKQDP EEEKMHFHNG HSKLPGVRTY IDPHTYEDPN 

       670        680        690        700        710        720 
QAVHEFAKEI EASCITIERV IGAGEFGEVC SGRLKLPGKR ELPVAIKTLK VGYTEKQRRD 

       730        740        750        760        770        780 
FLGEASIMGQ FDHPNIIHLE GVVTKSKPVM IVTEYMENGS LDTFLKKNDG QFTVIQLVGM 

       790        800        810        820        830        840 
LRGISAGMKY LSDMGYVHRD LAARNILINS NLVCKVSDFG LSRVLEDDPE AAYTTRGGKI 

       850        860        870        880        890        900 
PIRWTAPEAI AFRKFTSASD VWSYGIVMWE VVSYGERPYW EMTNQDVIKA VEEGYRLPSP 

       910        920        930        940        950        960 
MDCPAALYQL MLDCWQKERN SRPKFDEIVN MLDKLIRNPS SLKTLVNASC RVSNLLAEHS 

       970        980        990       1000       1010       1020 
PLGSGAYRSV GEWLEAIKMG RYTEIFMENG YSSMDAVAQV TLEDLRRLGV TLVGHQKKIM 

      1030 
NSLQEMKVQL VNGMVPL 

P54756 in FASTA format

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