[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE OF 1-6; 330-349 AND 613-632. STRAIN=ATCC 40221; PubMed=7626018 [NCBI, ExPASy, EBI, Israel, Japan] Long G.S., Bryant J.M., Taylor P.W., Luzio J.P.; "Complete nucleotide sequence of the gene encoding bacteriophage E endosialidase: implications for K1E endosialidase structure and function."; Biochem. J. 309:543-550(1995).
[2]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Gerardy-Schahn R., Hansen A., Brennecke T., Muehlenhoff M., Eckhardt M., Ziesing S., Lottspeich F., Frosch M.; Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases.
[3]	SEQUENCE REVISION TO 53-56; 523 AND 539. Muehlenhoff M.; Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: Responsible for initial absorption of the phage to the host bacterium. Degradation of the alpha-2,8-linked polysialic acid K1 capsule.
CATALYTIC ACTIVITY: Endohydrolysis of (2->8)-alpha-sialosyl linkages in oligo- or poly(sialic) acids.
SUBUNIT: Homotrimer.
PTM: The N-terminus is blocked.
SIMILARITY: Belongs to the glycosyl hydrolase 58 family [view classification].
SIMILARITY: Contains 3 BNR repeats.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X78310; CAA55120.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
Z36986; CAA85449.2; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

ModBase

P49714.

Ontologies

GO:0016996;	Molecular function: endo-alpha-sialidase activity (inferred from electronic annotation from EC).
GO:0008152;	Biological process: metabolic process (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR002860; BNR.
IPR001724; Glyco_hydro_58.
Graphical view of domain structure.

Pfam

PF02012; BNR; 2.
Pfam graphical view of domain structure.

PRINTS

PR00849; GLHYDRLASE58.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Direct protein sequencing; Glycosidase; Hydrolase; Repeat.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 811`	`811`	`Endo-N-acetylneuraminidase.`	`PRO_0000057708`
`REPEAT`	`150 161`	`12`	`BNR 1.`
`REPEAT`	`286 293`	`8`	`BNR 2.`
`REPEAT`	`398 409`	`12`	`BNR 3.`
`COMPBIAS`	`642 645`	`4`	`Poly-Ser.`

Sequence information

Length: 811 AA [This is the length of the unprocessed precursor]

Molecular weight: 90524 Da [This is the MW of the unprocessed precursor]

CRC64: 8F96F465ED533B6E [This is a checksum on the sequence]

        10         20         30         40         50         60 
MIQRLGSSLV KFKSKIAGAI WRNLDDKLTE VVSLKDFGAK GDGKTNDQDA VNAAMASGKR 

        70         80         90        100        110        120 
IDGAGATYKV SSLPDMERFY NTRFVWERLA GQPLYYVSKG FINGELYKIT DNPYYNAWPQ 

       130        140        150        160        170        180 
DKAFVYENVI YAPYMGSDRH GVSRLHVSWV KSGDDGQTWS TPEWLTDMHP DYPTVNYHCM 

       190        200        210        220        230        240 
SMGVCRNRLF AMIETRTLAK NELTNCALWD RPMSRSLHLT GGITKAANQR YATIHVPDHG 

       250        260        270        280        290        300 
LFVGDFVNFS NSAVTGVSGD MKVATVIDKD NFTVLTPNQQ TSDLNNAGKN WHMGTSFHKS 

       310        320        330        340        350        360 
PWRKTDLGLI PRVTEVHSFA TIDNNGFVMG YHQGDVAPRE VGLFYFPDAF NSPSNYVRRQ 

       370        380        390        400        410        420 
IPSEYEPDAA EPCIKYYDGV LYLITRGTRG DRLGSSLHRS RDIGQTWESL RFPHNVHHTT 

       430        440        450        460        470        480 
LPFAKVGDDL IMFGSERAEN EWEAGAPDDR YKASYPRTFY ARLNVNNWNA DDIEWVNITD 

       490        500        510        520        530        540 
QIYQGDIVNS SVGVGSVVVK DSFIYYIFGG ENHFNPMTYG DNKDKDPFKG HGHPTDIYCY 

       550        560        570        580        590        600 
KMQIANDNRV SRKFTYGATP GQAIPTFMGT DGIRNIPAPL YFSDNIVTED TKVGHLTLKA 

       610        620        630        640        650        660 
STSANIRSEM QMEGEYGFIG KSVPKDKPTG QRLIICGGEG TSSSSGAQIT LHGSNSSNAK 

       670        680        690        700        710        720 
RITYNGNEHL FQGAPIMPAV DNQFAAGGPS NRFTTIYLGS DPVTTSDADH KYGISSINTK 

       730        740        750        760        770        780 
VLKAWSRVGF KQYGLNSEAE RNLDSIHFGV LAQDIVAAFE AEGLDAIKYG IVSFEEGRYG 

       790        800        810 
VRYSEVLILE AAYTRHRLDK LEEMYATNKI S

P49714 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools