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UniProtKB/Swiss-Prot entry P48641


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name GSHRC_ARATH
Primary accession number P48641
Secondary accession numbers Q2V3T0 Q94BM6 Q9C5I4
Integrated into Swiss-Prot on February 1, 1996
Sequence was last modified on February 1, 1996 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 75)
Name and origin of the protein
Protein name Glutathione reductase, cytosolic
Synonyms GRase
GR
EC 1.8.1.7
OBP29
Gene name
OrderedLocusNames: At3g24170
ORFNames: MUJ8.7
From
Arabidopsis thaliana (Mouse-ear cress) [TaxID: 3702] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
Protein existence 2: Evidence at transcript level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA].
STRAIN=cv. Columbia;
TISSUE=Leaf;
Loebler M.;
"An Arabidopsis cDNA homologous to glutathione reductase.";
(er) Plant Gene Register PGR96-053.
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Columbia;
DOI=10.1093/dnares/7.2.131; PubMed=10819329 [NCBI, ExPASy, EBI, Israel, Japan]
Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
"Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence features of the regions of 4,504,864 bp covered by sixty P1 and TAC clones.";
DNA Res. 7:131-135(2000).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=cv. Columbia;
DOI=10.1126/science.1088305; PubMed=14593172 [NCBI, ExPASy, EBI, Israel, Japan]
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
"Empirical analysis of transcriptional activity in the Arabidopsis genome.";
Science 302:842-846(2003).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
U37697; AAB67841.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AB028621; BAB01358.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF360228; AAK25938.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY040029; AAK64087.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY140042; AAM98183.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY142628; AAN13086.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BT008870; AAP68309.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_001030756.2; -.
NP_001118688.1; -.
NP_189059.1; -.
UniGene At.24980
3D structure databases
HSSP Q94655; 1ONF. [HSSP ENTRY / PDB]
ModBase P48641.
Organism-specific databases
TAIR At3g24170; -.
Gene expression databases
ArrayExpress P48641; -.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from electronic annotation from InterPro).
GO:0009055; Molecular function: electron carrier activity (inferred from electronic annotation from InterPro).
GO:0050660; Molecular function: FAD binding (inferred from electronic annotation from InterPro).
GO:0004362; Molecular function: glutathione-disulfide reductase activity (inferred from electronic annotation from InterPro).
GO:0050661; Molecular function: NADP binding (inferred from electronic annotation from InterPro).
GO:0045454; Biological process: cell redox homeostasis (inferred from electronic annotation from InterPro).
GO:0006749; Biological process: glutathione metabolic process (inferred from electronic annotation from InterPro).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR013027; FAD_pyr_nucl-diS_OxRdtase.
IPR006324; Glut_reduct_pln.
IPR000815; Hg_reductase.
IPR001100; Pyr_nuc-diS_OxRdtase.
IPR004099; Pyr_nucl-diS_OxRdtase_dimer.
IPR012999; Pyr_OxRdtase_I_AS.
IPR001327; Pyr_OxRdtase_NAD_bd.
IPR001864; Trypnth_redctse.
Graphical view of domain structure.
Gene3D G3DSA:3.30.390.30; Pyr_redox_dim; 1.
Pfam PF00070; Pyr_redox; 1.
PF07992; Pyr_redox_2; 1.
PF02852; Pyr_redox_dim; 1.
Pfam graphical view of domain structure.
PRINTS PR00368; FADPNR.
PR00945; HGRDTASE.
PR00411; PNDRDTASEI.
PR00470; TRYPANRDTASE.
ProDom PD000139; FAD_pyr_redox; 1.
[Domain structure / List of seq. sharing at least 1 domain]
TIGRFAMs TIGR01424; gluta_reduc_2; 1.
PROSITE PS00076; PYRIDINE_REDOX_1; 1.
ProtoNet P48641.
Genome annotation databases
GeneID 822003; -.
GenomeReviews BA000014_GR; AT3G24170.
KEGG ath:AT3G24170; -.
NMPDR fig|3702.1.peg.14677; -.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Cytoplasm; FAD; Flavoprotein; NADP; Oxidoreductase; Redox-active center.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   499  499     Glutathione reductase, cytosolic. PRO_0000067961
NP_BIND   64    73  10     FAD (By similarity). 
ACT_SITE   472   472        Proton acceptor (By similarity). 
BINDING   234   234        NADP (By similarity). 
BINDING   240   240        NADP (By similarity). 
DISULFID   73    78        Redox-active (By similarity). 
CONFLICT   162   162        T -> A (in Ref. 3; AAK25938/AAK64087). 
Sequence information
Length: 499 AA [This is the length of the unprocessed precursor] Molecular weight: 53871 Da [This is the MW of the unprocessed precursor] CRC64: D6B29E8B5B867186 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MARKMLVDGE IDKVAADEAN ATHYDFDLFV IGAGSGGVRA ARFSANHGAK VGICELPFHP 

        70         80         90        100        110        120 
ISSEEIGGVG GTCVIRGCVP KKILVYGATY GGELEDAKNY GWEINEKVDF TWKKLLQKKT 

       130        140        150        160        170        180 
DEILRLNNIY KRLLANAAVK LYEGEGRVVG PNEVEVRQID GTKISYTAKH ILIATGSRAQ 

       190        200        210        220        230        240 
KPNIPGHELA ITSDEALSLE EFPKRAIVLG GGYIAVEFAS IWRGMGATVD LFFRKELPLR 

       250        260        270        280        290        300 
GFDDEMRALV ARNLEGRGVN LHPQTSLTQL TKTDQGIKVI SSHGEEFVAD VVLFATGRSP 

       310        320        330        340        350        360 
NTKRLNLEAV GVELDQAGAV KVDEYSRTNI PSIWAVGDAT NRINLTPVAL MEATCFANTA 

       370        380        390        400        410        420 
FGGKPTKAEY SNVACAVFCI PPLAVVGLSE EEAVEQATGD ILVFTSGFNP MKNTISGRQE 

       430        440        450        460        470        480 
KTLMKLIVDE KSDKVIGASM CGPDAAEIMQ GIAIALKCGA TKAQFDSTVG IHPSSAEEFV 

       490 
TMRSVTRRIA HKPKPKTNL 

P48641 in FASTA format

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