NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1104/pp.103.1.41; PubMed=8208857 [NCBI, ExPASy, EBI, Israel, Japan]
Burnett R.J., Maldonado-Mendoza I.E., McKnight T.D., Nessler C.L.;
"Expression of a 3-hydroxy-3-methylglutaryl coenzyme A reductase gene from Camptotheca acuminata is differentially regulated by wounding and methyl jasmonate.";
Plant Physiol. 103:41-48(1993).

Comments

FUNCTION: Catalyzes the synthesis of mevalonate. The specific precursor of all isoprenoid compounds present in plants.
CATALYTIC ACTIVITY: (R)-mevalonate + CoA + 2 NADP⁺ = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH.
PATHWAY: Metabolic intermediate biosynthesis; mevalonic acid biosynthesis; (R)-mevalonic acid from acetyl-CoA: step 3/3 .
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein.
SIMILARITY: Belongs to the HMG-CoA reductase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

L10390; AAA33040.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

HSSP

P04035; 1HWI. [HSSP ENTRY / PDB]

ModBase

P48021.

Ontologies

GO:0005789;	Cellular component: endoplasmic reticulum membrane (inferred from electronic annotation from UniProtKB-SubCell).
GO:0016021;	Cellular component: integral to membrane (inferred from electronic annotation from InterPro).
GO:0004420;	Molecular function: hydroxymethylglutaryl-CoA reductase (NADPH) activity (inferred from electronic annotation from InterPro).
GO:0050661;	Molecular function: NADP binding (inferred from electronic annotation from InterPro).
GO:0015936;	Biological process: coenzyme A metabolic process (inferred from electronic annotation from InterPro).
GO:0008299;	Biological process: isoprenoid biosynthetic process (inferred from electronic annotation from InterPro).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR002202; HMG_CoA_Rdtase_cat.
IPR004554; HMG_CoA_Rdtase_I_cat.
Graphical view of domain structure.

Gene3D

G3DSA:3.90.770.10; HMG-CoA_red; 1.

PANTHER

PTHR10572; HMG-CoA_red; 1.

Pfam

PF00368; HMG-CoA_red; 1.
Pfam graphical view of domain structure.

PRINTS

PR00071; HMGCOARDTASE.

TIGRFAMs

TIGR00533; HMG_CoA_R_NADP; 1.

PROSITE

PS00066; HMG_COA_REDUCTASE_1; 1.
PS00318; HMG_COA_REDUCTASE_2; 1.
PS01192; HMG_COA_REDUCTASE_3; 1.
PS50065; HMG_COA_REDUCTASE_4; 1.
PROSITE graphical view of domain structure (profiles).

ProtoNet

P48021.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Endoplasmic reticulum; Glycoprotein; Isoprene biosynthesis; Membrane; NADP; Oxidoreductase; Transmembrane.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 593`	`593`	`3-hydroxy-3-methylglutaryl-coenzyme A reductase.`	`PRO_0000114435`
`TRANSMEM`	`52 72`	`21`	`Potential.`
`TRANSMEM`	`94 114`	`21`	`Potential.`
`REGION`	`115 177`	`63`	`Linker (By similarity).`
`REGION`	`178 593`	`416`	`Catalytic (By similarity).`
`ACT_SITE`	`272 272`		`Charge relay system (By similarity).`
`ACT_SITE`	`404 404`		`Charge relay system (By similarity).`
`ACT_SITE`	`480 480`		`Charge relay system (By similarity).`
`ACT_SITE`	`578 578`		`Proton donor (By similarity).`
`CARBOHYD`	`336 336`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`449 449`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`582 582`		`N-linked (GlcNAc...) (Potential).`

Sequence information

Length: 593 AA [This is the length of the unprocessed precursor]

Molecular weight: 63278 Da [This is the MW of the unprocessed precursor]

CRC64: F22CB55B2E4944A7 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MDVRRRSINS IHQIPSVGGT APPMLKPKQP TKVDAVDLPD SPKASDALPL PLYITNGVFF 

        70         80         90        100        110        120 
TLFFTVVYYL LVRWREKIRN STPLHVVTLS EIAAIFTFVA SFIYLLGFFG IGLVQPFTSR 

       130        140        150        160        170        180 
SSHDDVWGVD DDEDVDEIVL KEDTRTVPCA AAPVDCPLPP IKPKVVDPVP ISPPSSEEDE 

       190        200        210        220        230        240 
EIIKSVVEGT TPSYALESKL GDSHRAAAIR REALQRMTKK SLAGLPLDGF DYDSILGQCC 

       250        260        270        280        290        300 
EMPVGYVQIP VGIAGPLLLD GREYSVPMAT TEGCLVASTN RGCKAIFACG GATSVLLRDA 

       310        320        330        340        350        360 
MTRAPVVRFG SAKRAADLKF FLENPLNFET LAAVFNSSSR FGKLQNIKCA IAGKNLYMRY 

       370        380        390        400        410        420 
SCSTGDAMGM NMISKGVQNV LDFLQDDFPD MDVIGISGNY CSDKKPAAVN WIEGRGKSVV 

       430        440        450        460        470        480 
CEAVIKEEVV KKVLKTNVAS LVELNMLKNL TGSAMAGALG GFNAHASNIV SAVYLATGQD 

       490        500        510        520        530        540 
PAQNVESSHC ITMMEAINDG KDLHVSVTMP SIEVGTVGGG TQLASQSACL NLLGVKGASK 

       550        560        570        580        590 
EAPGSNARLL ATIVAGSVLA GELSLMSAIA AGQLVNSHMK YNRSNKDVTK ASS

P48021 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools