NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Liver;
PubMed=7974347 [NCBI, ExPASy, EBI, Israel, Japan]
Sheffield W.P., Schuyler P.D., Blajchman M.A.;
"Molecular cloning and expression of rabbit heparin cofactor II: a plasma thrombin inhibitor highly conserved between species.";
Thromb. Haemost. 71:778-782(1994).

Comments

FUNCTION: Thrombin inhibitor activated by the glycosaminoglycans, heparin or dermatan sulfate. In the presence of the latter, HC-II becomes the predominant thrombin inhibitor in place of antithrombin III (AT).
DOMAIN: The N-terminal acidic repeat region mediates, in part, the glycosaminoglycan-accelerated thrombin inhibition (By similarity).
PTM: N-glycosylated; different glycan composition appears to lead to two forms of this protein (56 and 60 kDa).
SIMILARITY: Belongs to the serpin family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

S73493; AAB32401.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

I46990; I46990.

NP_001075798.1; -.

3D structure databases

HSSP

P05546; 1JMJ. [HSSP ENTRY / PDB]

SMR

P47776; 57-480.

ModBase

P47776.

Protein family/group databases

MEROPS

I04.019; -.

Ontologies

GO:0008201;	Molecular function: heparin binding (inferred from electronic annotation from UniProtKB-KW).
GO:0004867;	Molecular function: serine-type endopeptidase inhibitor activity (inferred from electronic annotation from InterPro).
GO:0007596;	Biological process: blood coagulation (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR000295; Prot_inh_Lserp2.
IPR000215; Protease_inhib_I4_serpin.
Graphical view of domain structure.

PANTHER

PTHR11461; Prot_inh_serpin; 1.

Pfam

PF00079; Serpin; 1.
Pfam graphical view of domain structure.

PRINTS

PR00780; LEUSERPINII.

SMART

SM00093; SERPIN; 1.
SMART graphical view of domain structure.

PROSITE

PS00284; SERPIN; 1.

ProtoNet

P47776.

Genome annotation databases

Ensembl

ENSOCUG00000009048; Oryctolagus cuniculus. [Contig view]

GeneID

100009170; -.

Phylogenomic databases

HOVERGEN

P47776; -.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Blood coagulation; Glycoprotein; Heparin-binding; Protease inhibitor; Repeat; Serine protease inhibitor; Signal; Sulfation.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`SIGNAL`	`1 19`	`19`	`Potential.`
`CHAIN`	`20 480`	`461`	`Heparin cofactor 2.`	`PRO_0000032496`
`REPEAT`	`56 66`	`11`	`1.`
`REPEAT`	`70 80`	`11`	`2.`
`REGION`	`56 80`	`25`	`2 X 11 AA approximate repeats, Asp/Glu-rich (acidic) (hirudin-like).`
`REGION`	`173 193`	`21`	`Glycosaminoglycan-binding site (By similarity).`
`SITE`	`444 445`	`2`	`Reactive bond (By similarity).`
`MOD_RES`	`62 62`		`Sulfotyrosine (By similarity).`
`MOD_RES`	`75 75`		`Sulfotyrosine (By similarity).`
`CARBOHYD`	`32 32`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`169 169`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`368 368`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`404 404`		`N-linked (GlcNAc...) (Potential).`

Sequence information

Length: 480 AA [This is the length of the unprocessed precursor]

Molecular weight: 54532 Da [This is the MW of the unprocessed precursor]

CRC64: 803BCF25203CA4FA [This is a checksum on the sequence]

        10         20         30         40         50         60 
MQHRPHLLLI SLTIMSVCGG SNGLTDQLNN KNLTMPLLPI EFHKENTVTN DWIPEGEEDD 

        70         80         90        100        110        120 
DYLDLEKLLS EDDDYIDIID AVSPTDSEAS AGNILQLFQG KSRIQRLNIL NAKFAFSLYR 

       130        140        150        160        170        180 
ALKDQANAFD NIFIAPVGIS TAMGMISLGL KGETHEQVHS VLHFRDFVNA SSKYEILTIH 

       190        200        210        220        230        240 
NLFRKLTHRL FRRNFGYTLR SVNDLYVQKQ FPIREDFKAK VREYYFAEAQ AADFSDPAFI 

       250        260        270        280        290        300 
SKANNHILKV TKGLIKEALE NVDPATQMMI LNCIYFKGTW VNKFPVEMTH NHNFRLNERE 

       310        320        330        340        350        360 
VVKVSMMQTK GNFLAANDQE LACDVLQLEY VGGISMLIVV PHKLSGMKTL EAQLTPQVVE 

       370        380        390        400        410        420 
RWQKSMTNRT REVLLPKFKL EKNYNLVEAL KSMGVTELFD KNGNMSGISD QGITMDLFKH 

       430        440        450        460        470        480 
QGTITVNEEG TQAAAVTTVG FMPLSTQVRF TVDRPFLFLV YEHRTSCLLF MGKVANPVRS

P47776 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools