[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. STRAIN=cv. Enrei; Kato T., Shirano Y., Iwamoto H., Shibata D.; "Soybean lipoxygenase L-4, a major component of the 94-kilodalton storage protein in vegetative tissues: expression and accumulation in leaves induced by pod removal and by methyl jasmonate."; Plant Cell Physiol. 34:1063-1072(1993).
[2]	PARTIAL PROTEIN SEQUENCE. TISSUE=Cotyledon; PubMed=16668631 [NCBI, ExPASy, EBI, Israel, Japan] Kato T., Ohta H., Tanaka K., Shibata D.; "Appearance of new lipoxygenases in soybean cotyledons after germination and evidence for expression of a major new lipoxygenase gene."; Plant Physiol. 98:324-330(1992).

Comments

FUNCTION: Plant lipoxygenase may be involved in a number of diverse aspects of plant physiology including growth and development, pest resistance, and senescence or responses to wounding. It catalyzes the hydroperoxidation of lipids, containing a cis,cis-1,4-pentadiene structure.
CATALYTIC ACTIVITY: Linoleate + O₂ = (9Z,11E)-(13S)-13-hydroperoxyoctadeca-9,11-dienoate.
COFACTOR: Binds 1 iron ion per subunit. Iron is tightly bound (By similarity).
PATHWAY: Lipid metabolism; oxylipin biosynthesis .
SUBUNIT: Monomer.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Found in maturing and developing seeds. In young seedlings it is found in cotyledons, hypocotyls, roots and primary leaves.
DEVELOPMENTAL STAGE: Developmentally regulated. Expression is high 3 to 5 days after germination and returns to basal level by day 9.
INDUCTION: By pod removal and methyl jasmonate.
SIMILARITY: Belongs to the lipoxygenase family.
SIMILARITY: Contains 1 lipoxygenase domain.
SIMILARITY: Contains 1 PLAT domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

D13999; BAA03101.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

T07662; T07662.

3D structure databases

HSSP

P09186; 1NO3. [HSSP ENTRY / PDB]

ModBase

P38417.

Ontologies

GO:0005737;	Cellular component: cytoplasm (inferred from electronic annotation from UniProtKB-KW).
GO:0009055;	Molecular function: electron carrier activity (inferred from electronic annotation from InterPro).
GO:0005506;	Molecular function: iron ion binding (inferred from electronic annotation from InterPro).
GO:0016165;	Molecular function: lipoxygenase activity (inferred from electronic annotation from InterPro).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
GO:0031408;	Biological process: oxylipin biosynthetic process (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR000907; LipOase.
IPR013819; LipOase_C.
IPR001024; LipOase_LH2.
IPR001246; LipOase_pln.
Graphical view of domain structure.

Gene3D

G3DSA:2.60.60.20; Lipase_LipOase; 1.

PANTHER

PTHR11771; LipOase; 1.

Pfam

PF00305; Lipoxygenase; 1.
PF01477; PLAT; 1.
Pfam graphical view of domain structure.

PRINTS

PR00087; LIPOXYGENASE.
PR00468; PLTLPOXGNASE.

SMART

SM00308; LH2; 1.
SMART graphical view of domain structure.

PROSITE

PS00711; LIPOXYGENASE_1; 1.
PS00081; LIPOXYGENASE_2; 1.
PS51393; LIPOXYGENASE_3; 1.
PS50095; PLAT; 1.
PROSITE graphical view of domain structure (profiles).

ProtoNet

P38417.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cytoplasm; Dioxygenase; Direct protein sequencing; Fatty acid biosynthesis; Iron; Lipid synthesis; Metal-binding; Oxidoreductase; Oxylipin biosynthesis.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 853`	`853`	`Lipoxygenase-4.`	`PRO_0000220720`
`DOMAIN`	`34 159`	`126`	`PLAT.`
`DOMAIN`	`162 853`	`692`	`Lipoxygenase.`
`METAL`	`513 513`		`Iron; catalytic (By similarity).`
`METAL`	`518 518`		`Iron; catalytic (By similarity).`
`METAL`	`704 704`		`Iron; catalytic (By similarity).`
`METAL`	`708 708`		`Iron; catalytic (By similarity).`
`METAL`	`853 853`		`Iron; via carboxylate; catalytic (By similarity).`

Sequence information

Length: 853 AA [This is the length of the unprocessed precursor]

Molecular weight: 96535 Da [This is the MW of the unprocessed precursor]

CRC64: 533175EE1CFB34FC [This is a checksum on the sequence]

        10         20         30         40         50         60 
MFPFGQKGQK IKGTMVVMQK NVLDINSITS VGGIVDQGLG FIGSAVDALT FAATKISIQL 

        70         80         90        100        110        120 
ISATKADGGK GKIGKSTNLR GKITLPTLGA GEQAYDVNFE WDSDFGIPGA FYIKNFMQNE 

       130        140        150        160        170        180 
FYLKSLILED IPNHGTIHFV CNSWVYNSKN YKTDRIFFAN NTYLPSETPA PLLKYREEEL 

       190        200        210        220        230        240 
KNVRGDGTGE RKEWDRIYDY DVYNDLGNPD SGDKYARPVL GGSALPYPRR ERTGRGKTRK 

       250        260        270        280        290        300 
DPNSEKPSDF VYLPRDEAFG HLKSSDFLAY GIKSVSQDVL PVLTDAFDGN ILSLEFDNFA 

       310        320        330        340        350        360 
EVHKLYEGGV TLPTNFLSKI APIPVIKEIF RTDGEQFLKY PPPKVMQVDK SAWMTDEEFA 

       370        380        390        400        410        420 
RETIAGLNPN VIKIIEEFPL SSKLDTQAYG DHTCIIAKEH LEPNLGGLTV EQAIQNKKLF 

       430        440        450        460        470        480 
ILDHHDYLIP YLRKINANTT KTYATRTIFF LKDDGTLTPL AIELSKPHPQ GEEYGPVSEV 

       490        500        510        520        530        540 
YVPASEGVEA YIWLLAKAYV VVNDACYHQI ISHWLSTHAI VEPFVIATNR QLSVVHPIYK 

       550        560        570        580        590        600 
LLFPHYRDTM NINSLARKAL VNADGIIEKT FLWGRYSMEM SAVIYKDWVF TDQALPNDLV 

       610        620        630        640        650        660 
KRGVAVKDPS APHGVRLLIE DYPYASDGLE IWDAIKSWVQ EYVSFYYKSD EELQKDPELQ 

       670        680        690        700        710        720 
AWWKELVEVG HGDLKDKPWW QKMQTREELV EASAILIWIA SALHAAVNFG QYPYGGLILN 

       730        740        750        760        770        780 
RPTISRRFMP EKGSPEYDAL AKNPEKEFLK TITGKKETLI DLTVIEILSR HASDEFYLGQ 

       790        800        810        820        830        840 
RDGGDYWTSD AGPLEAFKRF GKKLEEIEKK LIEKNKDETL RNRYGPAKMP YTLLYPSSEE 

       850 
GLTFRGIPNS ISI

P38417 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools