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UniProtKB/Swiss-Prot entry P38041


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name BOB1_YEAST
Primary accession number P38041
Secondary accession numbers None
Integrated into Swiss-Prot on October 1, 1994
Sequence was last modified on October 1, 1994 (Sequence version 1)
Annotations were last modified on    June 16, 2009 (Entry version 86)
Name and origin of the protein
Protein name Protein BOB1
Synonyms BEM1-binding protein
Growth inhibitory protein 7
Gene name
Name: BOI1
Synonyms: BOB1, GIN7
OrderedLocusNames: YBL085W
ORFNames: YBL0717
From
Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1083/jcb.133.4.879; PubMed=8666672 [NCBI, ExPASy, EBI, Israel, Japan]
Bender L., Lo H.S., Lee H., Kokojan V., Peterson V., Bender A.;
"Associations among PH and SH3 domain-containing proteins and Rho-type GTPases in Yeast.";
J. Cell Biol. 133:879-894(1996).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC 204508 / S288c;
DOI=10.1002/yea.320111112; PubMed=7502586 [NCBI, ExPASy, EBI, Israel, Japan]
Obermaier B., Gassenhuber J., Piravandi E., Domdey H.;
"Sequence analysis of a 78.6 kb segment of the left end of Saccharomyces cerevisiae chromosome II.";
Yeast 11:1103-1112(1995).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 204508 / S288c;
PubMed=7813418 [NCBI, ExPASy, EBI, Israel, Japan]
Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C., Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M., Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M., Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H., Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D., Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N., Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J., Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C., Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P., Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y., Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F., Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E., Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M., Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B., Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L., Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M., Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S., Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K., Mewes H.-W., Kleine K.;
"Complete DNA sequence of yeast chromosome II.";
EMBO J. 13:5795-5809(1994).
[4]
LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
DOI=10.1038/nature02046; PubMed=14562106 [NCBI, ExPASy, EBI, Israel, Japan]
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.;
"Global analysis of protein expression in yeast.";
Nature 425:737-741(2003).
[5]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-207; SER-209; SER-525; SER-590 AND SER-655, AND MASS SPECTROMETRY.
DOI=10.1074/mcp.M400219-MCP200; PubMed=15665377 [NCBI, ExPASy, EBI, Israel, Japan]
Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.;
"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway.";
Mol. Cell. Proteomics 4:310-327(2005).
[6]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-104; SER-209; SER-528; SER-644 AND THR-919, AND MASS SPECTROMETRY.
DOI=10.1021/pr060559j; PubMed=17330950 [NCBI, ExPASy, EBI, Israel, Japan]
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.;
"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae.";
J. Proteome Res. 6:1190-1197(2007).
[7]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-735, AND MASS SPECTROMETRY.
DOI=10.1073/pnas.0607084104; PubMed=17287358 [NCBI, ExPASy, EBI, Israel, Japan]
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.;
"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry.";
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007).
[8]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-737, AND MASS SPECTROMETRY.
DOI=10.1073/pnas.0701622104; PubMed=17563356 [NCBI, ExPASy, EBI, Israel, Japan]
Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H.;
"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases.";
Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007).
[9]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-104; SER-157; THR-158; SER-206; THR-207; SER-209; SER-528 AND SER-655, AND MASS SPECTROMETRY.
DOI=10.1074/mcp.M700468-MCP200; PubMed=18407956 [NCBI, ExPASy, EBI, Israel, Japan]
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
"A multidimensional chromatography technology for in-depth phosphoproteome analysis.";
Mol. Cell. Proteomics 7:1389-1396(2008).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
L31406; AAB08439.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X79489; CAA56021.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z35846; CAA84906.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S45444; S45444.
RefSeq NP_009468.1; -.
3D structure databases
HSSP O08967; 1FHX. [HSSP ENTRY / PDB]
ModBase P38041.
Protein-protein interaction databases
DIP DIP:2226N; -.
IntAct P38041; 41.
Organism-specific databases
CYGD YBL085w; -.
SGD S000000181; BOI1.
Yeast-GFP YBL085W.
Gene expression databases
ArrayExpress P38041; -.
GermOnline YBL085W; Saccharomyces cerevisiae.
Ontologies
GO
GO:0005935; Cellular component: cellular bud neck (inferred from direct assay from SGD).
GO:0043332; Cellular component: mating projection tip (inferred from direct assay from SGD).
GO:0042802; Molecular function: identical protein binding (inferred from physical interaction from IntAct).
GO:0005543; Molecular function: phospholipid binding (inferred from direct assay from SGD).
GO:0007118; Biological process: budding cell apical bud growth (inferred from genetic interaction from SGD).
GO:0007109; Biological process: cytokinesis, completion of separation (inferred from genetic interaction from SGD).
GO:0030010; Biological process: establishment of cell polarity (traceable author statement from SGD).
GO:0007266; Biological process: Rho protein signal transduction (traceable author statement from SGD).
QuickGo view.
Family and domain databases
InterPro IPR011993; PH_type.
IPR001849; Pleckstrin_homology.
IPR001660; SAM.
IPR011510; SAM_2.
IPR001452; SH3_domain.
Graphical view of domain structure.
Gene3D G3DSA:2.30.29.30; PH_type; 1.
Pfam PF00169; PH; 1.
PF07647; SAM_2; 1.
PF00018; SH3_1; 1.
Pfam graphical view of domain structure.
ProDom PD000066; SH3; 1.
[Domain structure / List of seq. sharing at least 1 domain]
SMART SM00233; PH; 1.
SM00454; SAM; 1.
SM00326; SH3; 1.
SMART graphical view of domain structure.
PROSITE PS50003; PH_DOMAIN; 1.
PS50105; SAM_DOMAIN; 1.
PS50002; SH3; 1.
PROSITE graphical view of domain structure (profiles).
Genome annotation databases
Ensembl YBL085W; Saccharomyces cerevisiae. [Contig view]
GeneID 852193; -.
GenomeReviews Y13134_GR; YBL085W.
KEGG sce:YBL085W; -.
NMPDR fig|4932.3.peg.154; -.
Phylogenomic databases
HOGENOM P38041; -.
OMA P38041; GSEYSSH.
Other
NextBio 970676; -.
ProtoNet P38041.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; Phosphoprotein; SH3 domain.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   980  980     Protein BOB1. PRO_0000064968
DOMAIN   13    77  65     SH3. 
DOMAIN   228   292  65     SAM. 
DOMAIN   776   895  120     PH. 
MOD_RES   104   104        Phosphoserine. 
MOD_RES   157   157        Phosphoserine. 
MOD_RES   158   158        Phosphothreonine. 
MOD_RES   206   206        Phosphoserine. 
MOD_RES   207   207        Phosphothreonine. 
MOD_RES   209   209        Phosphoserine. 
MOD_RES   525   525        Phosphoserine. 
MOD_RES   528   528        Phosphoserine. 
MOD_RES   590   590        Phosphoserine. 
MOD_RES   644   644        Phosphoserine. 
MOD_RES   655   655        Phosphoserine. 
MOD_RES   735   735        Phosphoserine. 
MOD_RES   737   737        Phosphoserine. 
MOD_RES   919   919        Phosphothreonine. 
Sequence information
Length: 980 AA [This is the length of the unprocessed precursor] Molecular weight: 109295 Da [This is the MW of the unprocessed precursor] CRC64: 09F1DD1F9EF30F36 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MSLEGNTLGK GAKSFPLYIA VNQYSKRMED ELNMKPGDKI KVITDDGEYN DGWYYGRNLR 

        70         80         90        100        110        120 
TKEEGLYPAV FTKRIAIEKP ENLHKSPTQE SGNSGVKYGN LNDSASNIGK VSSHQQENRY 

       130        140        150        160        170        180 
TSLKSTMSDI DKALEELRSG SVEQEVSKSP TRVPEVSTPQ LQDEQTLIQE KTRNEENTTH 

       190        200        210        220        230        240 
DSLFSSTADL NLSSESLKNI SKSNISTKSL EPSSESVRQL DLKMAKSWSP EEVTDYFSLV 

       250        260        270        280        290        300 
GFDQSTCNKF KEHQVSGKIL LELELEHLKE LEINSFGIRF QIFKEIRNIK SAIDSSSNKL 

       310        320        330        340        350        360 
DADYSTFAFE NQAAQLMPAA TVNRDEIQQQ ISSKCNKLSS ESSDRKSSSV TTELQRPSSV 

       370        380        390        400        410        420 
VVNPNFKLHD PAEQILDMTE VPNLFADKDI FESPGRAPKP PSYPSPVQPP QSPSFNNRYT 

       430        440        450        460        470        480 
NNNARFPPQT TYPPKNKNPT VYSNGLIPNS STSSDNSTGK FKFPAMNGHD SNSRKTTLTS 

       490        500        510        520        530        540 
ATIPSINTVN TDESLPAISN ISSNATSHHP NRNSVVYNNH KRTESGSSFV DLFNRISMLS 

       550        560        570        580        590        600 
PVKSSFDEEE TKQPSKASRA VFDSARRKSS YGHSRDASLS EMKKHRRNSS ILSFFSSKSQ 

       610        620        630        640        650        660 
SNPTSPTKQT FTIDPAKMTS HSRSQSNSYS HARSQSYSHS RKHSLVTSPL KTSLSPINSK 

       670        680        690        700        710        720 
SNIALAHSET PTSSNNKEAV SQPSEGKHKH KHKHKSKHKH KNSSSKDGSS EEKSKKKLFS 

       730        740        750        760        770        780 
STKESFVGSK EFKRSPSELT QKSTKSILPR SNAKKQQTSA FTEGIRSITA KESMQTADCS 

       790        800        810        820        830        840 
GWMSKKGTGA MGTWKQRFFT LHGTRLSYFT NTNDEKERGL IDITAHRVLP ASDDDRLISL 

       850        860        870        880        890        900 
YAASLGKGKY CFKLVPPQPG SKKGLTFTEP RVHYFAVENK SEMKAWLSAI IKATIDIDTS 

       910        920        930        940        950        960 
VPVISSYATP TIPLSKAQTL LEEARLQTQL RDAEEEEGRD QFGWDDTQNK RNSNYPIEQD 

       970        980 
QFETSDYLES SAFEYPGGRL 

P38041 in FASTA format

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