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UniProtKB/Swiss-Prot entry P32504


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name CBF3A_YEAST
Primary accession number P32504
Secondary accession numbers None
Integrated into Swiss-Prot on October 1, 1993
Sequence was last modified on October 1, 1993 (Sequence version 1)
Annotations were last modified on    June 16, 2009 (Entry version 74)
Name and origin of the protein
Protein name Centromere DNA-binding protein complex CBF3 subunit A
Synonyms Chromosome transmission fidelity protein 14
Kinetochore protein CTF14
Centromere-binding factor 2
Gene name
Name: CBF2
Synonyms: CBF3A, CEP2, CTF14, NDC10
OrderedLocusNames: YGR140W
From
Saccharomyces cerevisiae (Baker's yeast) [TaxID: 4932] 
Taxonomy Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
DOI=10.1083/jcb.121.3.513; PubMed=8486733 [NCBI, ExPASy, EBI, Israel, Japan]
Jiang W., Lechner J., Carbon J.;
"Isolation and characterization of a gene (CBF2) specifying a protein component of the budding yeast kinetochore.";
J. Cell Biol. 121:513-519(1993).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
DOI=10.1083/jcb.121.3.503; PubMed=8486732 [NCBI, ExPASy, EBI, Israel, Japan]
Goh P.-Y., Kilmartin J.V.;
"NDC10: a gene involved in chromosome segregation in Saccharomyces cerevisiae.";
J. Cell Biol. 121:503-512(1993).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 96604 / S288c / FY1679;
PubMed=9169869 [NCBI, ExPASy, EBI, Israel, Japan]
Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
Nature 387:81-84(1997).
[4]
INTERACTION WITH CTF19.
PubMed=10323865 [NCBI, ExPASy, EBI, Israel, Japan]
Ortiz J., Stemmann O., Rank S., Lechner J.;
"A putative protein complex consisting of Ctf19, Mcm21, and Okp1 represents a missing link in the budding yeast kinetochore.";
Genes Dev. 13:1140-1155(1999).
[5]
SUBCELLULAR LOCATION.
DOI=10.1083/jcb.146.2.415; PubMed=10427094 [NCBI, ExPASy, EBI, Israel, Japan]
Zeng X., Kahana J.A., Silver P.A., Morphew M.K., McIntosh J.R., Fitch I.T., Carbon J., Saunders W.S.;
"Slk19p is a centromere protein that functions to stabilize mitotic spindles.";
J. Cell Biol. 146:415-425(1999).
[6]
LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
DOI=10.1038/nature02046; PubMed=14562106 [NCBI, ExPASy, EBI, Israel, Japan]
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.;
"Global analysis of protein expression in yeast.";
Nature 425:737-741(2003).
[7]
ASSEMBLY OF THE CBF3 COMPLEX, AND INTERACTION WITH CBF3D AND SGT1.
DOI=10.1091/mbc.E03-12-0887; PubMed=15090617 [NCBI, ExPASy, EBI, Israel, Japan]
Rodrigo-Brenni M.C., Thomas S., Bouck D.C., Kaplan K.B.;
"Sgt1p and Skp1p modulate the assembly and turnover of CBF3 complexes required for proper kinetochore function.";
Mol. Biol. Cell 15:3366-3378(2004).
[8]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-566; TYR-837 AND TYR-890, AND MASS SPECTROMETRY.
DOI=10.1074/mcp.M700468-MCP200; PubMed=18407956 [NCBI, ExPASy, EBI, Israel, Japan]
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
"A multidimensional chromatography technology for in-depth phosphoproteome analysis.";
Mol. Cell. Proteomics 7:1389-1396(2008).
Comments
  • FUNCTION: Acts as a component of the centromere DNA-binding protein complex CBF3, which is essential for chromosome segregation and movement of centromeres along microtubules. CBF3 is required for the recruitment of other kinetochore complexes to CEN DNA. It plays a role in the attachment of chromosomes to the spindle and binds selectively to a highly conserved DNA sequence called CDEIII, found in centromers and in several promoters.
  • SUBUNIT: Component of the CBF3 copmplex, which is formed of CBF3A/CBF2, CBF3B/CEP3, CBF3C/CTF13 and CBF3D. CBF2 strongly interacts with BIR1. Interacts with the central kinetochore protein CTF19.
  • INTERACTION:
    P53267:DAM1; NbExp=1; IntAct=EBI-4069, EBI-23268;
  • SUBCELLULAR LOCATION: Nucleus. Centromere. Nucleus, spindle pole body. Note=Spindle midzone during anaphase B.
  • MISCELLANEOUS: Present with 1350 molecules/cell in log phase SD medium.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
Z21627; CAA79753.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X69300; CAA49158.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
Z72925; CAA97153.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR S64449; S64449.
RefSeq NP_011656.1; -.
3D structure databases
ModBase P32504.
Protein-protein interaction databases
DIP DIP:5955N; -.
IntAct P32504; 6.
Organism-specific databases
CYGD YGR140w; -.
SGD S000003372; CBF2.
Yeast-GFP YGR140W.
Gene expression databases
ArrayExpress P32504; -.
GermOnline YGR140W; Saccharomyces cerevisiae.
Ontologies
GO
GO:0031518; Cellular component: CBF3 complex (inferred from direct assay from SGD).
GO:0000778; Cellular component: condensed nuclear chromosome kinetochore (inferred from direct assay from SGD).
GO:0051233; Cellular component: spindle midzone (inferred from direct assay from SGD).
GO:0005816; Cellular component: spindle pole body (inferred from electronic annotation from UniProtKB-SubCell).
GO:0019237; Molecular function: centromeric DNA binding (inferred from direct assay from SGD).
GO:0008301; Molecular function: DNA bending activity (inferred from direct assay from SGD).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
GO:0007059; Biological process: chromosome segregation (inferred from mutant phenotype from SGD).
GO:0000022; Biological process: mitotic spindle elongation (inferred from mutant phenotype from SGD).
GO:0000921; Biological process: septin ring assembly (inferred from mutant phenotype from SGD).
QuickGo view.
Proteomic databases
PeptideAtlas P32504; -.
Genome annotation databases
Ensembl YGR140W; Saccharomyces cerevisiae. [Contig view]
GeneID 853041; -.
GenomeReviews Y13135_GR; YGR140W.
KEGG sce:YGR140W; -.
NMPDR fig|4932.3.peg.2775; -.
Phylogenomic databases
HOGENOM P32504; -.
OMA P32504; CVHSMNK.
Other
NextBio 972944; -.
ProtoNet P32504.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Centromere; Chromosomal protein; Complete proteome; Direct protein sequencing; DNA-binding; Nucleus; Phosphoprotein.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   956  956     Centromere DNA-binding protein complex CBF3 subunit A. PRO_0000089369
MOD_RES   566   566        Phosphoserine. 
MOD_RES   837   837        Phosphotyrosine. 
MOD_RES   890   890        Phosphotyrosine. 
CONFLICT   774   774        K -> Q (in Ref. 1; CAA79753). 
Sequence information
Length: 956 AA [This is the length of the unprocessed precursor] Molecular weight: 111917 Da [This is the MW of the unprocessed precursor] CRC64: C93FB7E033931E3D [This is a checksum on the sequence]
        10         20         30         40         50         60 
MRSSILFLLK LMKIMDVQQQ QEAMSSEDRF QELVDSLKPR TAHQYKTYYT KYIQWCQLNQ 

        70         80         90        100        110        120 
IIPTPEDNSV NSVPYKDLPI SAELIHWFLL DTLITDDKPG EKREETEDLD EEEENSFKIA 

       130        140        150        160        170        180 
TLKKIIGSLN FLSKLCKVHE NPNANIDTKY LESVTKLHTH WIDSQKAITT NETNNTNTQV 

       190        200        210        220        230        240 
LCPPLLKVSL NLWNPETNHL SEKFFKTCSE KLRFLVDFQL RSYLNLSFEE RSKIRFGSLK 

       250        260        270        280        290        300 
LGKRDRDAII YHKVTHSAEK KDTPGHHQLL ALLPQDCPFI CPQTTLAAYL YLRFYGIPSV 

       310        320        330        340        350        360 
SKGDGFPNLN ADENGSLLQD IPILRGKSLT TYPREETFSN YYTTVFRYCH LPYKRREYFN 

       370        380        390        400        410        420 
KCNLVYPTWD EDTFRTFFNE ENHGNWLEQP EAFAFPDKIP FDFKKIMNFK SPYTSYSTNA 

       430        440        450        460        470        480 
KKDPFPPPKD LLVQIFPEID EYKRHDYEGL SQNSRDFLDL MEVLRERFLS NLPWIYKFFP 

       490        500        510        520        530        540 
NHDIFQDPIF GNSDFQSYFN DKTIHSKGSP ILSFDILPGF NKIYKNKTNF YSLLIERPSQ 

       550        560        570        580        590        600 
LTFASSHNPD THPTQKQESE GPLQMSQLDT TQLNELLKQQ SFEYVQFQTL SNFQILLSVF 

       610        620        630        640        650        660 
NKIFEKLEMK KSSRGYILHQ LNLFKITLDE RIKKSKIDDA DKFIRDNQPI KKEENIVNED 

       670        680        690        700        710        720 
GPNTSRRTKR PKQIRLLSIA DSSDESSTED SNVFKKDGES IEDGAYGENE DENDSEMQEQ 

       730        740        750        760        770        780 
LKSMINELIN SKISTFLRDQ MDQFELKINA LLDKILEEKV TRIIEQKLGS HTGKFSTLKR 

       790        800        810        820        830        840 
PQLYMTEEHN VGFDMEVPKK LRTSGKYAET VKDNDDHQAM STTASPSPEQ DQEAKSYTDE 

       850        860        870        880        890        900 
QEFMLDKSID SIEGIILEWF TPNAKYANQC VHSMNKSGNK SWRANCEALY KERKSIVEFY 

       910        920        930        940        950 
IYLVNHESLD RYKAVDICEK LRDQNEGSFS RLAKFLRKWR HDHQNSFDGL LVYLSN 

P32504 in FASTA format

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