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UniProtKB/Swiss-Prot entry P24658

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name L_CDVO
Primary accession number P24658
Secondary accession numbers None
Integrated into Swiss-Prot on March 1, 1992
Sequence was last modified on May 30, 2000 (Sequence version 3)
Annotations were last modified on    November 4, 2008 (Entry version 52)
Name and origin of the protein
Protein name Large structural protein
Synonyms Protein L
Transcriptase
Replicase
Includes RNA-directed RNA polymerase
     (EC 2.7.7.48)
mRNA (guanine-N(7)-)-methyltransferase
     (EC 2.1.1.56)
mRNA guanylyltransferase
     (EC 2.7.7.-)
Gene name
Name: L
From
Canine distemper virus (strain Onderstepoort) (CDV) [TaxID: 11233] 
Taxonomy Viruses; ssRNA negative-strand viruses; Mononegavirales; Paramyxoviridae; Paramyxovirinae; Morbillivirus.
Virus hosts Ailuropoda melanoleuca (Giant panda) [TaxID: 9646]
Ailurus fulgens (Lesser panda) (Red panda) [TaxID: 9649]
Canis familiaris (Dog) [TaxID: 9615]
Mustela [TaxID: 9665]
Panthera leo (Lion) [TaxID: 9689]
Procyon lotor (Raccoon) [TaxID: 9654]
Zalophus californianus (California sealion) [TaxID: 9704]
Protein existence 3: Inferred from homology;
References
[1]
 NUCLEOTIDE SEQUENCE [GENOMIC RNA].
DOI=10.1006/viro.1993.1102; PubMed=8438585 [NCBI, ExPASy, EBI, Israel, Japan]
Sidhu M.S., Menonna J.P., Cook S.D., Dowling P.C., Udem S.A.;
"Canine distemper virus L gene: sequence and comparison with related viruses.";
Virology 193:50-65(1993).
[2]
 SEQUENCE REVISION.
Sidhu M.S.;
Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases.
[3]
 NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 1-63.
PubMed=1993883 [NCBI, ExPASy, EBI, Israel, Japan]
Curran M.D., Clarke D.K., Rima B.K.;
"The nucleotide sequence of the gene encoding the attachment protein H of canine distemper virus.";
J. Gen. Virol. 72:443-447(1991).
Comments
  • FUNCTION: Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently the subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The transcriptase stutters on a specific sequence, resulting on a cotranscriptional editing of the phosphoprotein (P) mRNA. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals (By similarity).
  • CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
  • CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
  • SUBUNIT: Interacts with the P protein (By similarity).
  • SUBCELLULAR LOCATION: Virion (Potential). Cytoplasm (By similarity).
  • SIMILARITY: Belongs to the paramyxoviruses L protein family.
  • SIMILARITY: Contains 1 RdRp catalytic domain.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AF014953; AAC26996.1; -; Genomic_RNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
D00758; BAA00655.1; -; Genomic_RNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A45389; A45389.
RefSeq NP_047207.1; -.
3D structure databases
ModBase P24658.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from electronic annotation from UniProtKB-KW).
GO:0019012; Cellular component: virion (inferred from electronic annotation from UniProtKB-KW).
GO:0005524; Molecular function: ATP binding (inferred from electronic annotation from UniProtKB-KW).
GO:0004482; Molecular function: mRNA (guanine-N7-)-methyltransferase activity (inferred from electronic annotation from EC).
GO:0006370; Biological process: mRNA capping (inferred from electronic annotation from UniProtKB-KW).
GO:0006410; Biological process: transcription, RNA-dependent (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR016269; RNA-dir_RNA_pol_paramyxovir.
IPR014023; RNA_pol_cat.
IPR001016; RNA_pol_L_viral.
Graphical view of domain structure.
Pfam PF00946; Paramyx_RNA_pol; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF000830; RNA_pol_ParamyxoV; 1.
PROSITE PS50526; RDRP_SSRNA_NEG_NONSEG; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS P24658.
ProtoNet P24658.
Genome annotation databases
GeneID 1489795; -.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
ATP-binding; Cytoplasm; Methyltransferase; mRNA capping; mRNA processing; Multifunctional enzyme; Nucleotide-binding; Nucleotidyltransferase; RNA replication; RNA-directed RNA polymerase; S-adenosyl-L-methionine; Transferase; Virion.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom    To Length Description FTId
CHAIN   1   2184  2184     Large structural protein. PRO_0000142726
DOMAIN   656    840  185     RdRp catalytic. 
NP_BIND   1785   1794  10     ATP (Potential). 
CONFLICT   44     44        T -> R (in Ref. 3; BAA00655). 
Sequence information
Length: 2184 AA [This is the length of the unprocessed precursor] Molecular weight: 248190 Da [This is the MW of the unprocessed precursor] CRC64: BEF45756BBF0D37D [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MDSVSVNQIL YPEVHLDSPI VTNKLVSILE YARIRHNYQL LDTTLVRNIK ERISEGFSNQ 

        70         80         90        100        110        120 
MIINCIEIGS IINQTLLSYP KHNHVIYPNC NKLLFHAQDR VISLRLRNIF KRGNSIYSKI 

       130        140        150        160        170        180 
TDGVKKCLND INLNIGLGGA LDKTIGTKID EAGIIMQSSQ WFEPFLLWFT IKTEMRSVIK 

       190        200        210        220        230        240 
SSTHNCRKRR QNPVFVKGES LNVLVSRDLV CIIDLTSHIV YYLTFEMVLM YCDVIEGRLM 

       250        260        270        280        290        300 
TDTAMAIDQR YSTLHVRIRY LWDLIDGFFP DLGNSTYQLV ALLEPLSLAY LQLKDITFSL 

       310        320        330        340        350        360 
RGAFLSHCFA EIQEILQDNG FYTEETFQTL TQALDFVFIT EDIHITGEIF SFFRSFGHPR 

       370        380        390        400        410        420 
LEAITAAENV RKHMNQPKVV SYETMMKGHA IFCGIIINGY RDRHGGTWPP MDLPVHASPI 

       430        440        450        460        470        480 
IRNAHASGEG ITYSQCIENW KSFAGIRFKC FMPLSLDSDL TMYLKDKALA ALRKEWDSVY 

       490        500        510        520        530        540 
PKEFLRYNPP RSTESRRLVN VFLEDSQFDP YNMIMYVISG QYLEDPDFNL SYSLKEKEIK 

       550        560        570        580        590        600 
EVGRLFAKMT YKMRACQVIA ENLISNGIGK YFKDNGMAKD EHDLTKSLHT LAVSGVPKDK 

       610        620        630        640        650        660 
KDSHRGLTNQ RKSLKPAPYR GTRHSVSSPS SRYIDPNPNF CTSRREDNDI EIYETVSAFI 

       670        680        690        700        710        720 
TTDLKKYCLN WRYETISIFA QRLNEIYGLP SFFQWLHRRL EQSILYVSDP HCPPDLDRHV 

       730        740        750        760        770        780 
DLNTAPNSQI FIKYPMGGVE GYCQKLWTIS TIPYLYLAAH ESGVRIASLV QGDNQTIAVT 

       790        800        810        820        830        840 
KRVPSTWSYA LKKSEASRVT TEYFIALRQR LHDVGHHLKA NETIISSHFF VYSKGIYYDG 

       850        860        870        880        890        900 
MLISQSLKSI ARCVFWSETI VDETRAACSN ISTTLAKAIE KGFDRYLAYT LNILKIIQQV 

       910        920        930        940        950        960 
LISLGFTINS AMTRDVIEPL LQDHCLLTKM AILPAPIGGF NYLNMSRLFV RNIGDPVTSS 

       970        980        990       1000       1010       1020 
IADLKRMIRS GLLGVEILHQ VMTQYPGDSS YLDWASDPYS ANLPCVQSIT RLLKNITARH 

      1030       1040       1050       1060       1070       1080 
VLINSPNPML RGLFHDESQD EDEALAAFLM DRKIIIPRAA HEILDNTITG AREAIAGMLD 

      1090       1100       1110       1120       1130       1140 
TTKGLIRASM KRGGLTPRII TRLSTYDYEQ FRAGIRLFSG KGHDQLIDQD SCSVQLARAL 

      1150       1160       1170       1180       1190       1200 
RNHMWAKLAK GRPIYGLEVP DILESMKGYM IRRHESCLLC ASGSHNYGWF FIPANCQLDS 

      1210       1220       1230       1240       1250       1260 
ITEGTSALRV PYIGSTTEER TDMKLAFVKS PSRSLKSAVR IATVYSWAYG DDDESWQEAW 

      1270       1280       1290       1300       1310       1320 
TLAKQRADIS LEELRMITPI STSTNLAHRL RDKSTQVKYS GTSLIRVARY ATISNDNLSF 

      1330       1340       1350       1360       1370       1380 
IIDDKKVDTN FIYQQGMLLG LGILEHLFRL SSTTGDSNTV LHLHVETDCC VIPMSDHPRV 

      1390       1400       1410       1420       1430       1440 
PGLRKVVIPR NICTNPLIYD SNPIIEKDAV RLYNQSHRKH IVEFVTWTTG QLYHVLAKST 

      1450       1460       1470       1480       1490       1500 
AMSMVEMITK FEKDHLNEVT ALIGDDDINS FITEFLLVEP RLFTVYLGQC AAINWGFEIH 

      1510       1520       1530       1540       1550       1560 
YHRPSGKYQM GELLFSFLSR MSKGVFKILA NALSHPKVYR RFWDSGMIEP VHGPSLDSQN 

      1570       1580       1590       1600       1610       1620 
LHITVCNLIY NCYMIYLDLL LNDELDDFSF ILCESDEDVI PERFDNIQAR HLCILSDLYC 

      1630       1640       1650       1660       1670       1680 
NPRDCPQIRG LTPTQKCAVL SGYLKSKALE SHVGLTWNDK PILIDQYSCS LTYLRRGSIK 

      1690       1700       1710       1720       1730       1740 
QIRLRVDPGF ITDAVGCLER RPLRNNSTSK ASELTSGFDP PKDDLAKLLS QLSTRTHNLP 

      1750       1760       1770       1780       1790       1800 
ITGLGVRNYE VHSFRRIGIN STACYKAVEI ASVIKNEFTS EEHGLFLGEG SGAMLTVYKE 

      1810       1820       1830       1840       1850       1860 
LLRLSRCYYN SGVSVESRTG QREISPYPSE VSLVEHQLGL DKLVTVLFNG RPEVTWVGSV 

      1870       1880       1890       1900       1910       1920 
DCYKYILSQI SASSLGLIHS DIESLPDKDI IEKLEELSAI LSMTLILGKV GSVLVIKIMP 

      1930       1940       1950       1960       1970       1980 
VSGDWVQGFI LYALPHFLRS FIVYPRYSNF VSTEAYLVFT GLRAGRLINP EGIKQQILRV 

      1990       2000       2010       2020       2030       2040 
GIRTSPGLVG HILSSKQTAC VQSLHGPPFH AKSFNPHLQG LTSIEKVLIN CGLTINGLKV 

      2050       2060       2070       2080       2090       2100 
CKNLLHHDIS SGEEGLKGSI TILYRELARF KDNHQSSHGM FHAYPVLIAS QERELVSIIA 

      2110       2120       2130       2140       2150       2160 
KKYCGYILLY SGDLYEITRI VRNLKANHII FDLHRNLFMD NLSRSDRSLI LTTIPKKNWL 

      2170       2180 
FQLETKEIKE WFKLLGYSAL IRNH
P24658 in FASTA format

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