ID   RIP6_SAPOF              Reviewed;         299 AA.
AC   P20656; Q41392;
DT   01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 2.
DT   22-JUL-2008, entry version 68.
DE   RecName: Full=Ribosome-inactivating protein saporin-6;
DE            Short=SAP-6;
DE            Short=SO-6;
DE            EC=3.2.2.22;
DE   AltName: Full=rRNA N-glycosidase;
DE   Flags: Precursor;
GN   Name=SAP6;
OS   Saponaria officinalis (Common soapwort).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   Caryophyllales; Caryophyllaceae; Saponaria.
OX   NCBI_TaxID=3572;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 1-283, AND PROTEIN SEQUENCE OF 25-115;
RP   206-213 AND 234-277.
RC   TISSUE=Leaf;
RX   MEDLINE=89338421; PubMed=2547612;
RA   Benatti L., Saccardo M.B., Dani M., Nitti G., Sassano M.,
RA   Lorenzetti R., Lappi D.A., Soria M.;
RT   "Nucleotide sequence of cDNA coding for saporin-6, a type-1 ribosome-
RT   inactivating protein from Saponaria officinalis.";
RL   Eur. J. Biochem. 183:465-470(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 25-277.
RX   MEDLINE=93203250; PubMed=8454624;
RA   Barthelemy I., Martineau D., Ong M., Matsunami R., Ling N.,
RA   Benatti L., Cavallaro U., Soria M., Lappi D.A.;
RT   "The expression of saporin, a ribosome-inactivating protein from the
RT   plant Saponaria officinalis, in Escherichia coli.";
RL   J. Biol. Chem. 268:6541-6548(1993).
RN   [3]
RP   NUCLEOTIDE SEQUENCE OF 275-299.
RC   TISSUE=Leaf;
RX   MEDLINE=92038053; PubMed=1936274; DOI=10.1016/0014-5793(91)81303-P;
RA   Benatti L., Nitti G., Solinas M., Valsasina B., Vitale A.,
RA   Ceriotti A., Soria M.R.;
RT   "A Saporin-6 cDNA containing a precursor sequence coding for a
RT   carboxyl-terminal extension.";
RL   FEBS Lett. 291:285-288(1991).
RN   [4]
RP   PROTEIN SEQUENCE OF 25-61.
RX   MEDLINE=85251709; PubMed=3925952; DOI=10.1016/0006-291X(85)91981-3;
RA   Lappi D.A., Esch F.S., Barbieri L., Stirpe F., Soria M.;
RT   "Characterization of a Saponaria officinalis seed ribosome-
RT   inactivating protein: immunoreactivity and sequence homologies.";
RL   Biochem. Biophys. Res. Commun. 129:934-942(1985).
RN   [5]
RP   PROTEIN SEQUENCE OF 25-72 AND 114-154.
RX   MEDLINE=90220515; PubMed=2325629; DOI=10.1007/BF00280379;
RA   Fordham-Skelton A.P., Yarwood A., Croy R.R.D.;
RT   "Synthesis of saporin gene probes from partial protein sequence data:
RT   use of inosine-oligonucleotides, genomic DNA and the polymerase chain
RT   reaction.";
RL   Mol. Gen. Genet. 221:134-138(1990).
RN   [6]
RP   X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 25-277, AND ACTIVE SITE.
RX   PubMed=10745075;
RA   Savino C., Federici L., Ippoliti R., Lendaro E., Tsernoglou D.;
RT   "The crystal structure of saporin SO6 from Saponaria officinalis and
RT   its interaction with the ribosome.";
RL   FEBS Lett. 470:239-243(2000).
CC   -!- FUNCTION: Ribosome-inactivating protein of type 1, inhibits
CC       protein synthesis in animal cells. Useful as immunotoxin for
CC       pharmacological applications.
CC   -!- CATALYTIC ACTIVITY: Endohydrolysis of the N-glycosidic bond at one
CC       specific adenosine on the 28S rRNA.
CC   -!- TISSUE SPECIFICITY: Seeds and leaves of the plant.
CC   -!- SIMILARITY: Belongs to the ribosome-inactivating protein family.
CC       Type 1 RIP subfamily.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; X15655; CAA33685.1; -; mRNA.
DR   EMBL; S57638; AAB25863.1; -; mRNA.
DR   EMBL; X69135; CAA48889.1; -; Genomic_DNA.
DR   EMBL; X64917; CAA46110.1; -; mRNA.
DR   PIR; S05205; S05205.
DR   PIR; S16487; S16487.
DR   PIR; S18307; S18307.
DR   PIR; S29931; S29931.
DR   PIR; S38527; S38527.
DR   PDB; 1QI7; X-ray; 2.00 A; A=25-277.
DR   PDBsum; 1QI7; -.
DR   SMR; P20656; 25-277.
DR   InterPro; IPR001574; Ribosome_inactivat_prot.
DR   InterPro; IPR016138; Ribosome_inactivat_prot_sub1.
DR   InterPro; IPR016139; Ribosome_inactivat_prot_sub2.
DR   Gene3D; G3DSA:3.40.420.10; Ribosome_inactivat_prot_sub1; 1.
DR   Gene3D; G3DSA:4.10.470.10; Ribosome_inactivat_prot_sub2; 1.
DR   Pfam; PF00161; RIP; 1.
DR   PRINTS; PR00396; SHIGARICIN.
DR   PROSITE; PS00275; SHIGA_RICIN; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; Glycoprotein; Hydrolase;
KW   Plant defense; Protein synthesis inhibitor; Signal; Toxin.
FT   SIGNAL        1     24
FT   CHAIN        25    277       Ribosome-inactivating protein saporin-6.
FT                                /FTId=PRO_0000030776.
FT   PROPEP      278    299       Potential.
FT                                /FTId=PRO_0000030777.
FT   ACT_SITE    200    200
FT   CARBOHYD    283    283       N-linked (GlcNAc...) (Potential).
FT   VARIANT      72     72       E -> D.
FT   VARIANT     115    115       R -> K.
FT   CONFLICT     60     60       G -> S (in Ref. 4; AA sequence).
FT   CONFLICT    123    123       S -> L (in Ref. 2 and 5).
FT   CONFLICT    212    212       I -> T (in Ref. 1; AA sequence).
SQ   SEQUENCE   299 AA;  33607 MW;  4BC312958BB4E79B CRC64;
     MKIYVVATIA WILLQFSAWT TTDAVTSITL DLVNPTAGQY SSFVDKIRNN VKDPNLKYGG
     TDIAVIGPPS KEKFLRINFQ SSRGTVSLGL KRDNLYVVAY LAMDNTNVNR AYYFRSEITS
     AESTALFPEA TTANQKALEY TEDYQSIEKN AQITQGDQSR KELGLGIDLL STSMEAVNKK
     ARVVKDEARF LLIAIQMTAE AARFRYIQNL VIKNFPNKFN SENKVIQFEV NWKKISTAIY
     GDAKNGVFNK DYDFGFGKVR QVKDLQMGLL MYLGKPKSSN EANSTVRHYG PLKPTLLIT
//