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UniProtKB/Swiss-Prot entry P15209


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name NTRK2_MOUSE
Primary accession number P15209
Secondary accession numbers Q3TUF9 Q80WU0 Q91XJ9
Integrated into Swiss-Prot on April 1, 1990
Sequence was last modified on April 1, 1990 (Sequence version 1)
Annotations were last modified on    June 16, 2009 (Entry version 113)
Name and origin of the protein
Protein name BDNF/NT-3 growth factors receptor [Precursor]
Synonyms EC 2.7.10.1
Neurotrophic tyrosine kinase receptor type 2
TrkB tyrosine kinase
GP145-TrkB/GP95-TrkB
Trk-B
Gene name
Name: Ntrk2
Synonyms: Trkb
From
Mus musculus (Mouse) [TaxID: 10090] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Mus.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM GP145-TRKB).
TISSUE=Brain;
PubMed=2555172 [NCBI, ExPASy, EBI, Israel, Japan]
Klein R., Parada L.F., Coulier F., Barbacid M.;
"trkB, a novel tyrosine protein kinase receptor expressed during mouse neural development.";
EMBO J. 8:3701-3709(1989).
[2]
NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS GP145-TRKB AND GP95-TRKB).
TISSUE=Brain;
DOI=10.1016/0092-8674(90)90476-U; PubMed=2160854 [NCBI, ExPASy, EBI, Israel, Japan]
Klein R., Conway D., Parada L.F., Barbacid M.;
"The trkB tyrosine protein kinase gene codes for a second neurogenic receptor that lacks the catalytic kinase domain.";
Cell 61:647-656(1990).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS GP145-TRKB AND GP95-TRKB).
STRAIN=C57BL/6J, and NOD;
TISSUE=Thymus;
DOI=10.1126/science.1112014; PubMed=16141072 [NCBI, ExPASy, EBI, Israel, Japan]
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J., Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
"The transcriptional landscape of the mammalian genome.";
Science 309:1559-1563(2005).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM GP95-TRKB).
STRAIN=C57BL/6;
TISSUE=Brain;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[5]
PARTIAL NUCLEOTIDE SEQUENCE (ISOFORMS L1 AND L10).
TISSUE=Trigeminal ganglion;
DOI=10.1074/jbc.272.20.13019; PubMed=9148911 [NCBI, ExPASy, EBI, Israel, Japan]
Ninkina N., Grashchuck M., Buchman V.L., Davies A.M.;
"TrkB variants with deletions in the leucine-rich motifs of the extracellular domain.";
J. Biol. Chem. 272:13019-13025(1997).
[6]
FUNCTION.
DOI=10.1016/0092-8674(91)90396-G; PubMed=1645620 [NCBI, ExPASy, EBI, Israel, Japan]
Soppet D., Escandon E., Maragos J., Middlemas D.S., Reid S.W., Blair J., Burton L.E., Stanton B.R., Kaplan D.R., Hunter T., Nicolics K., Parada L.F.;
"The neurotrophic factors brain-derived neurotrophic factor and neurotrophin-3 are ligands for the trkB tyrosine kinase receptor.";
Cell 65:895-903(1991).
[7]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-515, AND MASS SPECTROMETRY.
TISSUE=Brain;
DOI=10.1021/pr0701254; PubMed=18034455 [NCBI, ExPASy, EBI, Israel, Japan]
Ballif B.A., Carey G.R., Sunyaev S.R., Gygi S.P.;
"Large-scale identification and evolution indexing of tyrosine phosphorylation sites from murine brain.";
J. Proteome Res. 7:311-318(2008).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M33385; AAA40482.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X17647; CAA35636.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK018789; BAB31412.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK147391; BAE27880.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK160789; BAE36012.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC052014; AAH52014.2; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
IPI IPI00128360; -.
IPI00229333; -.
IPI00229334; -.
IPI00270098; -.
PIR A35104; A35104.
S06943; S06943.
RefSeq NP_001020245.1; -.
NP_032771.1; -.
UniGene Mm.130054
3D structure databases
HSSP Q16620; 1WWB. [HSSP ENTRY / PDB]
ModBase P15209.
Protein-protein interaction databases
DIP DIP:5722N; -.
IntAct P15209; 1.
PTM databases
PhosphoSite P15209; -.
Enzyme and pathway databases
BRENDA 2.7.10.1; 244.
Organism-specific databases
MGI MGI:97384; Ntrk2.
Gene expression databases
ArrayExpress P15209; -.
Bgee P15209; -.
CleanEx MM_NTRK2; -.
GermOnline ENSMUSG00000055254; Mus musculus.
Ontologies
GO
GO:0005829; Cellular component: cytosol (inferred from direct assay from MGI).
GO:0016021; Cellular component: integral to membrane (inferred from electronic annotation from UniProtKB-KW).
GO:0005886; Cellular component: plasma membrane (inferred from direct assay from MGI).
GO:0043195; Cellular component: terminal button (inferred from direct assay from MGI).
GO:0005524; Molecular function: ATP binding (inferred from electronic annotation from UniProtKB-KW).
GO:0004714; Molecular function: transmembrane receptor protein tyrosine kinase activity (inferred from electronic annotation from EC).
GO:0007631; Biological process: feeding behavior (inferred from mutant phenotype from MGI).
GO:0014047; Biological process: glutamate secretion (inferred from physical interaction from MGI).
GO:0042490; Biological process: mechanoreceptor differentiation (inferred from mutant phenotype from MGI).
GO:0019222; Biological process: regulation of metabolic process (inferred from mutant phenotype from MGI).
GO:0046548; Biological process: retinal rod cell development (inferred from mutant phenotype from MGI).
GO:0001570; Biological process: vasculogenesis (inferred from mutant phenotype from MGI).
QuickGo view.
Family and domain databases
InterPro IPR007110; Ig-like.
IPR013783; Ig-like_fold.
IPR013098; Ig_I-set.
IPR003598; Ig_sub2.
IPR000372; Leu-rich_rpt_Cys-rich-reg_N.
IPR000483; LRR_C.
IPR000719; Prot_kinase_core.
IPR017441; Protein_kinase_ATP_BS.
IPR002011; Recept_tyr_kinase-II_CS.
IPR001245; Tyr_pkinase.
IPR008266; Tyr_pkinase_AS.
Graphical view of domain structure.
Gene3D G3DSA:2.60.40.10; Ig-like_fold; 1.
Pfam PF07679; I-set; 1.
PF01462; LRRNT; 1.
PF07714; Pkinase_Tyr; 1.
Pfam graphical view of domain structure.
PRINTS PR00109; TYRKINASE.
ProDom PD000001; Prot_kinase; 1.
[Domain structure / List of seq. sharing at least 1 domain]
SMART SM00408; IGc2; 1.
SM00082; LRRCT; 1.
SM00013; LRRNT; 1.
SM00219; TyrKc; 1.
SMART graphical view of domain structure.
PROSITE PS50835; IG_LIKE; 1.
PS00107; PROTEIN_KINASE_ATP; 1.
PS50011; PROTEIN_KINASE_DOM; 1.
PS00109; PROTEIN_KINASE_TYR; 1.
PS00239; RECEPTOR_TYR_KIN_II; 1.
PROSITE graphical view of domain structure (profiles).
Proteomic databases
PRIDE P15209; -.
Genome annotation databases
Ensembl ENSMUSG00000055254; Mus musculus. [Contig view]
GeneID 18212; -.
KEGG mmu:18212; -.
Phylogenomic databases
HOGENOM P15209; -.
HOVERGEN P15209; -.
OMA P15209; DKAGREH.
Other
NextBio 293608; -.
SOURCE Ntrk2; Mus musculus.
ProtoNet P15209.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Alternative splicing; ATP-binding; Developmental protein; Differentiation; Disulfide bond; Glycoprotein; Immunoglobulin domain; Kinase; Leucine-rich repeat; Membrane; Neurogenesis; Nucleotide-binding; Phosphoprotein; Receptor; Repeat; Signal; Transferase; Transmembrane; Tyrosine-protein kinase.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
SIGNAL   1    31  31      
CHAIN   32   821  790     BDNF/NT-3 growth factors receptor. PRO_0000016728
TOPO_DOM   32   429  398     Extracellular (Potential). 
TRANSMEM   430   453  24     Potential. 
TOPO_DOM   454   821  368     Cytoplasmic (Potential). 
REPEAT   72    93  22     LRR 1. 
REPEAT   96   117  22     LRR 2. 
DOMAIN   197   282  86     Ig-like C2-type 1. 
DOMAIN   301   365  65     Ig-like C2-type 2. 
DOMAIN   537   806  270     Protein kinase. 
NP_BIND   543   551  9     ATP (By similarity). 
ACT_SITE   675   675        Proton acceptor (By similarity). 
BINDING   571   571        ATP (By similarity). 
SITE   515   515  1     Interaction with SHC1 (By similarity). 
SITE   816   816  1     Interaction with PLC-gamma-1 (By similarity). 
MOD_RES   515   515        Phosphotyrosine; by autocatalysis (By similarity). 
MOD_RES   701   701        Phosphotyrosine; by autocatalysis (By similarity). 
MOD_RES   705   705        Phosphotyrosine; by autocatalysis (By similarity). 
MOD_RES   706   706        Phosphotyrosine; by autocatalysis (By similarity). 
MOD_RES   816   816        Phosphotyrosine; by autocatalysis (By similarity). 
CARBOHYD   67    67        N-linked (GlcNAc...) (Potential). 
CARBOHYD   95    95        N-linked (GlcNAc...) (Potential). 
CARBOHYD   121   121        N-linked (GlcNAc...) (Potential). 
CARBOHYD   178   178        N-linked (GlcNAc...) (Potential). 
CARBOHYD   205   205        N-linked (GlcNAc...) (Potential). 
CARBOHYD   241   241        N-linked (GlcNAc...) (Potential). 
CARBOHYD   254   254        N-linked (GlcNAc...) (Potential). 
CARBOHYD   280   280        N-linked (GlcNAc...) (Potential). 
CARBOHYD   325   325        N-linked (GlcNAc...) (Potential). 
CARBOHYD   338   338        N-linked (GlcNAc...) (Potential). 
CARBOHYD   411   411        N-linked (GlcNAc...) (Potential). 
DISULFID   32    38        By similarity. 
DISULFID   36    45        By similarity. 
DISULFID   152   176        By similarity. 
DISULFID   154   194        By similarity. 
DISULFID   218   266        By similarity. 
DISULFID   302   345        By similarity. 
VAR_SEQ   71    71        I -> M (in isoform L10). VSP_002905
VAR_SEQ   72   143        Missing (in isoform L10). VSP_002906
VAR_SEQ   72   120        Missing (in isoform L1). VSP_002907
VAR_SEQ   466   476        PASVISNDDDS -> FVLFHKIPLDG (in isoform GP95-TRKB). VSP_002908
VAR_SEQ   477   821        Missing (in isoform GP95-TRKB). VSP_002909
Sequence information
Length: 821 AA [This is the length of the unprocessed precursor] Molecular weight: 92133 Da [This is the MW of the unprocessed precursor] CRC64: 50E08D5FF86D8F30 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MSPWLKWHGP AMARLWGLCL LVLGFWRASL ACPTSCKCSS ARIWCTEPSP GIVAFPRLEP 

        70         80         90        100        110        120 
NSVDPENITE ILIANQKRLE IINEDDVEAY VGLRNLTIVD SGLKFVAYKA FLKNSNLRHI 

       130        140        150        160        170        180 
NFTRNKLTSL SRRHFRHLDL SDLILTGNPF TCSCDIMWLK TLQETKSSPD TQDLYCLNES 

       190        200        210        220        230        240 
SKNMPLANLQ IPNCGLPSAR LAAPNLTVEE GKSVTLSCSV GGDPLPTLYW DVGNLVSKHM 

       250        260        270        280        290        300 
NETSHTQGSL RITNISSDDS GKQISCVAEN LVGEDQDSVN LTVHFAPTIT FLESPTSDHH 

       310        320        330        340        350        360 
WCIPFTVRGN PKPALQWFYN GAILNESKYI CTKIHVTNHT EYHGCLQLDN PTHMNNGDYT 

       370        380        390        400        410        420 
LMAKNEYGKD ERQISAHFMG RPGVDYETNP NYPEVLYEDW TTPTDIGDTT NKSNEIPSTD 

       430        440        450        460        470        480 
VADQSNREHL SVYAVVVIAS VVGFCLLVML LLLKLARHSK FGMKGPASVI SNDDDSASPL 

       490        500        510        520        530        540 
HHISNGSNTP SSSEGGPDAV IIGMTKIPVI ENPQYFGITN SQLKPDTFVQ HIKRHNIVLK 

       550        560        570        580        590        600 
RELGEGAFGK VFLAECYNLC PEQDKILVAV KTLKDASDNA RKDFHREAEL LTNLQHEHIV 

       610        620        630        640        650        660 
KFYGVCVEGD PLIMVFEYMK HGDLNKFLRA HGPDAVLMAE GNPPTELTQS QMLHIAQQIA 

       670        680        690        700        710        720 
AGMVYLASQH FVHRDLATRN CLVGENLLVK IGDFGMSRDV YSTDYYRVGG HTMLPIRWMP 

       730        740        750        760        770        780 
PESIMYRKFT TESDVWSLGV VLWEIFTYGK QPWYQLSNNE VIECITQGRV LQRPRTCPQE 

       790        800        810        820 
VYELMLGCWQ REPHTRKNIK SIHTLLQNLA KASPVYLDIL G 

P15209 in FASTA format

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