[1]	NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM MAP2B). STRAIN=Wistar; TISSUE=Brain; DOI=10.1093/nar/18.9.2822; PubMed=2339070 [NCBI, ExPASy, EBI, Israel, Japan] Kindler S., Schwanke B., Schulz B., Garner C.C.; "Complete cDNA sequence encoding rat high and low molecular weight MAP2."; Nucleic Acids Res. 18:2822-2822(1990).
[2]	NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM MAP2B). STRAIN=Wistar; TISSUE=Brain; PubMed=2174050 [NCBI, ExPASy, EBI, Israel, Japan] Kindler S., Schulz B., Goedert M., Garner C.C.; "Molecular structure of microtubule-associated protein 2b and 2c from rat brain."; J. Biol. Chem. 265:19679-19684(1990).
[3]	NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM MAP2C). DOI=10.1093/nar/18.2.361; PubMed=2326166 [NCBI, ExPASy, EBI, Israel, Japan] Doll T., Papandrikopoulou A., Matus A.; "Nucleotide and amino acid sequences of embryonic rat MAP2c."; Nucleic Acids Res. 18:361-361(1990).
[4]	DISCUSSION OF SEQUENCE. DOI=10.1038/340650a0; PubMed=2770869 [NCBI, ExPASy, EBI, Israel, Japan] Papandrikopoulou A., Doll T., Tucker R.P., Garner C.C., Matus A.; "Embryonic MAP2 lacks the cross-linking sidearm sequences and dendritic targeting signal of adult MAP2."; Nature 340:650-652(1989).
[5]	NUCLEOTIDE SEQUENCE [MRNA] OF 1695-1725 (ISOFORM MAP2X). PubMed=8282767 [NCBI, ExPASy, EBI, Israel, Japan] Doll T., Meichsner M., Riederer B.M., Honegger P., Matus A.; "An isoform of microtubule-associated protein 2 (MAP2) containing four repeats of the tubulin-binding motif."; J. Cell Sci. 106:633-640(1993).
[6]	PHOSPHORYLATION. PubMed=11029056 [NCBI, ExPASy, EBI, Israel, Japan] Ozer R.S., Halpain S.; "Phosphorylation-dependent localization of microtubule-associated protein MAP2c to the actin cytoskeleton."; Mol. Biol. Cell 11:3573-3587(2000).
[7]	PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-1619 AND THR-1622, AND MASS SPECTROMETRY. TISSUE=Kidney; DOI=10.1073/pnas.0600895103; PubMed=16641100 [NCBI, ExPASy, EBI, Israel, Japan] Hoffert J.D., Pisitkun T., Wang G., Shen R.-F., Knepper M.A.; "Quantitative phosphoproteomics of vasopressin-sensitive renal cells: regulation of aquaporin-2 phosphorylation at two sites."; Proc. Natl. Acad. Sci. U.S.A. 103:7159-7164(2006).

Comments

FUNCTION: The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton (Probable).

ALTERNATIVE PRODUCTS: 4 named isoforms [FASTA] produced by alternative splicing. Additional isoforms seem to exist.

Name	MAP2x
Isoform ID	P15146-1
This is the isoform sequence displayed in this entry.

Name	MAP2b
Isoform ID	P15146-2
Features which should be applied to build the isoform sequence: VSP_003199.

Name	MAP2c
Isoform ID	P15146-3
Features which should be applied to build the isoform sequence: VSP_003198, VSP_003199.

Name	MAP2d
Isoform ID	P15146-4
Features which should be applied to build the isoform sequence: VSP_003198.

DEVELOPMENTAL STAGE: Isoform MAP2C is expressed during embryonic brain development and until postanatal day 10. Isoform MAP2B is expressed throughout brain development.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR (By similarity). MAP2A/c is phosphorylated.
SIMILARITY: Contains 4 Tau/MAP repeats.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X51842; CAA36135.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
X17682; CAA35667.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
X71487; CAA50588.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

IPI

IPI00206171; -.
IPI00231051; -.
IPI00231052; -.
IPI00231053; -.

PIR

A37981; A37981.
I55502; S33176.

UniGene

Rn.10484

3D structure databases

DP00122; -.

PTM databases

P15146; -.

Organism-specific databases

RGD

3044; Mtap2.

Gene expression databases

ArrayExpress

P15146; -.

GermOnline

ENSRNOG00000011841; Rattus norvegicus.

Ontologies

GO:0005737;	Cellular component: cytoplasm (inferred from electronic annotation from UniProtKB-SubCell).
GO:0030425;	Cellular component: dendrite (inferred from direct assay from RGD).
GO:0005874;	Cellular component: microtubule (inferred from electronic annotation from UniProtKB-KW).
GO:0043234;	Cellular component: protein complex (inferred from direct assay from RGD).
GO:0019718;	Cellular component: rough microsome (inferred from direct assay from RGD).
GO:0019719;	Cellular component: smooth microsome (inferred from direct assay from RGD).
GO:0005516;	Molecular function: calmodulin binding (inferred from electronic annotation from UniProtKB-KW).
GO:0007026;	Biological process: negative regulation of microtubule depolymerization (inferred from electronic annotation from InterPro).
QuickGo view.

Family and domain databases

InterPro

IPR013588; MAP2_projctn.
IPR018459; RII_binding_1.
IPR001084; Tau_tubulin-bd.
Graphical view of domain structure.

Pfam

PF08377; MAP2_projctn; 1.
PF10522; RII_binding_1; 1.
PF00418; Tubulin-binding; 4.
Pfam graphical view of domain structure.

PROSITE

PS00229; TAU_MAP; 3.

Proteomic databases

PRIDE

P15146; -.

Genome annotation databases

Ensembl

ENSRNOG00000011841; Rattus norvegicus. [Contig view]

Phylogenomic databases

HOVERGEN

P15146; -.

Other

ProtoNet

P15146.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Alternative splicing; Calmodulin-binding; Cytoplasm; Cytoskeleton; Microtubule; Phosphoprotein; Repeat.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 1861`	`1861`	`Microtubule-associated protein 2.`	`PRO_0000072749`
`REPEAT`	`1664 1694`	`31`	`Tau/MAP motif 1.`
`REPEAT`	`1695 1725`	`31`	`Tau/MAP motif 2.`
`REPEAT`	`1726 1756`	`31`	`Tau/MAP motif 3.`
`REPEAT`	`1757 1788`	`32`	`Tau/MAP motif 4.`
`REGION`	`1454 1474`	`21`	`Calmodulin-binding (Potential).`
`MOD_RES`	`63 63`		`Phosphoserine (By similarity).`
`MOD_RES`	`610 610`		`Phosphoserine (By similarity).`
`MOD_RES`	`628 628`		`Phosphoserine (By similarity).`
`MOD_RES`	`657 657`		`Phosphoserine (By similarity).`
`MOD_RES`	`732 732`		`Phosphoserine (By similarity).`
`MOD_RES`	`739 739`		`Phosphoserine (By similarity).`
`MOD_RES`	`748 748`		`Phosphotyrosine (By similarity).`
`MOD_RES`	`825 825`		`Phosphoserine (By similarity).`
`MOD_RES`	`837 837`		`Phosphoserine (By similarity).`
`MOD_RES`	`1162 1162`		`Phosphoserine (By similarity).`
`MOD_RES`	`1353 1353`		`Phosphoserine (By similarity).`
`MOD_RES`	`1359 1359`		`Phosphothreonine (By similarity).`
`MOD_RES`	`1427 1427`		`Phosphoserine (By similarity).`
`MOD_RES`	`1446 1446`		`Phosphothreonine (By similarity).`
`MOD_RES`	`1487 1487`		`Phosphoserine (By similarity).`
`MOD_RES`	`1541 1541`		`Phosphoserine (By similarity).`
`MOD_RES`	`1597 1597`		`Phosphoserine (By similarity).`
`MOD_RES`	`1600 1600`		`Phosphothreonine (By similarity).`
`MOD_RES`	`1601 1601`		`Phosphothreonine (By similarity).`
`MOD_RES`	`1608 1608`		`Phosphothreonine (By similarity).`
`MOD_RES`	`1611 1611`		`Phosphothreonine (By similarity).`
`MOD_RES`	`1614 1614`		`Phosphoserine (By similarity).`
`MOD_RES`	`1617 1617`		`Phosphoserine (By similarity).`
`MOD_RES`	`1619 1619`		`Phosphothreonine.`
`MOD_RES`	`1622 1622`		`Phosphothreonine.`
`MOD_RES`	`1652 1652`		`Phosphothreonine (By similarity).`
`MOD_RES`	`1682 1682`		`Phosphoserine; by PKA.`
`MOD_RES`	`1744 1744`		`Phosphoserine; by PKA.`
`MOD_RES`	`1776 1776`		`Phosphoserine; by PKA.`
`MOD_RES`	`1816 1816`		`Phosphoserine (By similarity).`
`MOD_RES`	`1824 1824`		`Phosphoserine (By similarity).`
`MOD_RES`	`1832 1832`		`Phosphoserine (By similarity).`
`MOD_RES`	`1833 1833`		`Phosphoserine (By similarity).`
`MOD_RES`	`1834 1834`		`Phosphoserine (By similarity).`
`MOD_RES`	`1836 1836`		`Phosphoserine (By similarity).`
`VAR_SEQ`	`152 1514`		`Missing (in isoform MAP2c and isoform MAP2d).`	`VSP_003198`
`VAR_SEQ`	`1695 1725`		`Missing (in isoform MAP2b and isoform MAP2c).`	`VSP_003199`

Sequence information

Length: 1861 AA [This is the length of the unprocessed precursor]

Molecular weight: 202411 Da [This is the MW of the unprocessed precursor]

CRC64: 42DCF116D21EF54E [This is a checksum on the sequence]

        10         20         30         40         50         60 
MADERKDEGK APHWTSASLT EAAAHPHSPE MKDQGGSGEG LSRSANGFPY REEEEGAFGE 

        70         80         90        100        110        120 
HGSQGTYSDT KENGINGELT SADRETAEEV SARIVQVVTA EAVAVLKGEQ EKEAQHKDQP 

       130        140        150        160        170        180 
AALPLAAEET VNLPPSPPPS PASEQTAALE EDLLTASKME FPEQQKLPSS FAEPLDKEET 

       190        200        210        220        230        240 
EFKMQSKPGE DFEHAALVPQ PDTSKTPQDK KDPQDMEGEK SPASPFAQTF GTNLEDIKQI 

       250        260        270        280        290        300 
TEPSITVPSI GLSAEPLAPK DQKDWFIEMP VESKKDEWGL AAPISPGPLT PMREKDVLED 

       310        320        330        340        350        360 
IPRWEGKQFD SPMPSPFHGG SFTLPLDTVK DERVTEGSQP FAPVFFQSDD KMSLQDTSGS 

       370        380        390        400        410        420 
ATSKESSKDE EPQKDKADKV ADVPVSEATT VLGDVHSPAV EGFVGENISG EEKGTTDQEK 

       430        440        450        460        470        480 
KETSTPSVQE PTLTETEPQT KLEETSKVSI EETVAKEEES LKLKDDKAGV IQTSTEQSFS 

       490        500        510        520        530        540 
KEDQKGQEQT IEALKQDSFP ISLEQAVTDA AMATKTLEKV TSEPEAVSEK REIQGLFEED 

       550        560        570        580        590        600 
IADKSKLEGA GSATVAEVEM PFYEDKSGMS KYFETSALKE DVTRSTGLGS DYYELSDSRG 

       610        620        630        640        650        660 
NAQESLDTVS PKNQQDEKEL LAKASQPSPP AHEAGYSTLA QSYTSDHPSE LPEEPSSPQE 

       670        680        690        700        710        720 
RMFTIDPKVY GEKRDLHSKN KDDLTLSRSL GLGGRSAIEQ RSMSINLPMS CLDSIALGFN 

       730        740        750        760        770        780 
FGRGHDLSPL ASDILTNTSG SMDEGDDYLP PTTPAVEKIP CFPIESKEEE DKTEQAKVTG 

       790        800        810        820        830        840 
GQTTQVETSS ESPFPAKEYY KNGTVMAPDL PEMLDLAGTR SRLASVSADA EVARRKSVPS 

       850        860        870        880        890        900 
EAVVAESSTG LPPVADDSQP VKPDSQLEDM GYCVFNKYTV PLPSPVQDSE NLSGESGSFY 

       910        920        930        940        950        960 
EGTDDKVRRD LATDLSLIEV KLAAAGRVKD EFTAEKEASP PSSADKSGLS REFDQDRKAN 

       970        980        990       1000       1010       1020 
DKLDTVLEKS EEHVDSKEHA KESEEVGDKV ELFGLGVTYE QTSAKELITT KETAPERAEK 

      1030       1040       1050       1060       1070       1080 
GLSSVPEVAE VETTTKADQG LDVAAKKDDQ SPLDIKVSDF GQMASGMSVD AGKTIELKFE 

      1090       1100       1110       1120       1130       1140 
VDQQLTLSSE APQETDSFMG IESSHVKDGA KVSETEVKEK VAKPDLVHQE AVDKEESYES 

      1150       1160       1170       1180       1190       1200 
SGEHESLTME SLKPDEGKKE TSPETSLIQD EVALKLSVEI PCPPPVSEAD SSIDEKAEVQ 

      1210       1220       1230       1240       1250       1260 
MEFIQLPKEE STETPDIPAI PSDVTQPQPE AVVSEPAEVR GEEEEIEAEG EYDKLLFRSD 

      1270       1280       1290       1300       1310       1320 
TLQITDLLVP GSREEFVETC PGEHKGVVES VVTIEDDFIT VVQTTTDEGE LGSHSVRFAA 

      1330       1340       1350       1360       1370       1380 
PVQPEEERRP YPHDEELEVL MAAEAQAEPK DGSPDAPATP EKEEVPFSEY KTETYDDYKD 

      1390       1400       1410       1420       1430       1440 
ETTIDDSIMD ADSLWVDTQD DDRSILTEQL ETIPKEERAE KEARRPSLEK HRKEKPFKTG 

      1450       1460       1470       1480       1490       1500 
RGRISTPERR EVAKKEPSTV SRDEVRRKKA VYKKAELAKE SEVQAHSPSR KLILKPAIKY 

      1510       1520       1530       1540       1550       1560 
TRPTHLSCVK RKTTATSGES AQAPSAFKQA KDKVTDGITK SPEKRSSLPR PSSILPPRRG 

      1570       1580       1590       1600       1610       1620 
VSGDREENSF SLNSSISSAR RTTRSEPIRR AGKSGTSTPT TPGSTAITPG TPPSYSSRTP 

      1630       1640       1650       1660       1670       1680 
GTPGTPSYPR TPGTPKSGIL VPSEKKVAII RTPPKSPATP KQLRLINQPL PDLKNVKSKI 

      1690       1700       1710       1720       1730       1740 
GSTDNIKYQP KGGQVRILNK KMDFSKVQSR CGSKDNIKHS AGGGNVQIVT KKIDLSHVTS 

      1750       1760       1770       1780       1790       1800 
KCGSLKNIRH RPGGGRVKIE SVKLDFKEKA QAKVGSLDNA HHVPGGGNVK IDSQKLNFRE 

      1810       1820       1830       1840       1850       1860 
HAKARVDHGA EIITQSPSRS SVASPRRLSN VSSSGSINLL ESPQLATLAE DVTAALAKQG 


L

P15146 in FASTA format

View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools