RNA-directed RNA polymerase
     (EC 2.7.7.48)
mRNA (guanine-N(7)-)-methyltransferase
     (EC 2.1.1.56)
mRNA guanylyltransferase
     (EC 2.7.7.-)

Gene name

Name:

From

Chandipura virus (strain I653514) (CHPV)

[TaxID: 11273]

Taxonomy

Viruses; ssRNA negative-strand viruses; Mononegavirales; Rhabdoviridae; Dimarhabdovirus supergroup; Vesiculovirus.

Virus hosts

Homo sapiens (Human) [TaxID: 9606]
Phlebotominae (sandflies) [TaxID: 7198]

Protein existence

3: Inferred from homology;

References

[1]	NUCLEOTIDE SEQUENCE [GENOMIC RNA]. DOI=10.1007/s00705-004-0452-2; PubMed=15614433 [NCBI, ExPASy, EBI, Israel, Japan] Marriott A.C.; "Complete genome sequences of Chandipura and Isfahan vesiculoviruses."; Arch. Virol. 150:671-680(2005).
[2]	NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 1-11. DOI=10.1016/0042-6822(89)90540-0; PubMed=2741347 [NCBI, ExPASy, EBI, Israel, Japan] Masters P.S., Bhella R.S., Butcher M., Patel B., Ghosh H.P., Banerjee A.K.; "Structure and expression of the glycoprotein gene of Chandipura virus."; Virology 171:285-290(1989).

Comments

FUNCTION: Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently five subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals (By similarity).
CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m₇G(5')pppR-RNA.
SUBUNIT: Interacts with the P protein (By similarity).
SUBCELLULAR LOCATION: Virion (Potential). Cytoplasm (By similarity).
SIMILARITY: Belongs to the rhabdoviridae protein L family.
SIMILARITY: Contains 1 RdRp catalytic domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AJ810083; CAH17543.1; -; Genomic_RNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
J04350; AAA42917.1; -; Genomic_RNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

ModBase

P13179.

Ontologies

GO:0005737;	Cellular component: cytoplasm (inferred from electronic annotation from UniProtKB-KW).
GO:0019012;	Cellular component: virion (inferred from electronic annotation from UniProtKB-KW).
GO:0005524;	Molecular function: ATP binding (inferred from electronic annotation from UniProtKB-KW).
GO:0004482;	Molecular function: mRNA (guanine-N7-)-methyltransferase activity (inferred from electronic annotation from EC).
GO:0003723;	Molecular function: RNA binding (inferred from electronic annotation from InterPro).
GO:0003968;	Molecular function: RNA-directed RNA polymerase activity (inferred from electronic annotation from InterPro).
GO:0006370;	Biological process: mRNA capping (inferred from electronic annotation from UniProtKB-KW).
GO:0006410;	Biological process: transcription, RNA-dependent (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR017234; RNA-dir_RNA_pol_rhabdovir.
IPR014023; RNA_pol_cat.
IPR001016; RNA_pol_L_viral.
Graphical view of domain structure.

Pfam

PF00946; Paramyx_RNA_pol; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF037546; RNA_pol_RhabdoV_sub; 1.

PROSITE

PS50526; RDRP_SSRNA_NEG_NONSEG; 1.
PROSITE graphical view of domain structure (profiles).

ProtoNet

P13179.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

ATP-binding; Cytoplasm; Methyltransferase; mRNA capping; mRNA processing; Multifunctional enzyme; Nucleotide-binding; Nucleotidyltransferase; RNA replication; RNA-directed RNA polymerase; S-adenosyl-L-methionine; Transferase; Virion.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 2092`	`2092`	`Large structural protein.`	`PRO_0000222839`
`DOMAIN`	`588 774`	`187`	`RdRp catalytic.`

Sequence information

Length: 2092 AA [This is the length of the unprocessed precursor]

Molecular weight: 238532 Da [This is the MW of the unprocessed precursor]

CRC64: AE9091D6CFF0B206 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MDLNPVDDAA ELSEENFFSG KLSKECRIRG LNSVDYNLNS PLVSDDLTYL LDKFKGKPVP 

        70         80         90        100        110        120 
IRWKMKKWDS ILDQLRKHDL EYLRPSDLHQ WFAEWMLYSK HGSKQGEDFL KTVDEEASDT 

       130        140        150        160        170        180 
FEVVRSFIRG WTGGEINFVR KSGKHMGYCA ELCQKFLDLH KLTLLGNAAT DNELLQLSKT 

       190        200        210        220        230        240 
FGDDKIYKKR LIKLPSLGRV IFDSGFFIVL DQRVLMDRNF MLMMKDVIIG RMQTVLSMIS 

       250        260        270        280        290        300 
RCDDKFSSKD IDFLLKVYST GDKIIRKLGN DGYELIKTVE PMCNLRLSDL ARRFRPLIPP 

       310        320        330        340        350        360 
FPHFRRHIES TVDELSAKTP LIRELFSLID TSPNVDSTLV VYGSFRHWGH PFINYFEGLE 

       370        380        390        400        410        420 
KLHKQVTMEK EIDTNYSEAL ASDLARIVLT KEFNEKKQWA VDYHRVPTNH PFKNHIRDNT 

       430        440        450        460        470        480 
WPTAAVIQDF GDHWHELPLI QCFDIPDLID PSIIYSDKSH SMNRSEVLNH VRTKPHTPIP 

       490        500        510        520        530        540 
SKKVLESMID KPATNWLEFL EEIDKNGLSD EDLVIGLKGK ERELKIAGRF FSLMSWKLRE 

       550        560        570        580        590        600 
YFVVTEYLIK THFVPLFHGL TMADDMTAVI KKMLESSSGQ GLTNYESVCI ANHIDYEKWN 

       610        620        630        640        650        660 
NHQRKLSNGP VFKVMGQFLG YPNLIYRTHE FFEKSLIYYN ERPDLMKVKN GILENSTHQR 

       670        680        690        700        710        720 
VCWNGQAGGL EGLRQKGWSI LNLLVIQREA KIRNTAVKVL AQGDNQVICT QYKTKQYRND 

       730        740        750        760        770        780 
IELRQALNQM AANNDVIMKA IESGTNKLGL LINQDETMQS ADYLNYGKVP IFRGVIRGLE 

       790        800        810        820        830        840 
TKRWSRVTCV TNDQLPTCAN LMSSVSTNAL TVAHFDVHPL NAMIQFNFFG NFARLLLIMH 

       850        860        870        880        890        900 
DPAIRQSLNQ LKGPNINVHS YGFKVAMLYL DPSIGGVCGT ALSRFLIRSF PDPVTESLSF 

       910        920        930        940        950        960 
WKLIHHSTSD IRLKNLSEQF GNPKIAVFRE SHIEKLLEDP TSLNISMGMS AANLLKTEIK 

       970        980        990       1000       1010       1020 
KNLLQKKSSI GNQIVKDAVY YIHSEDEKLR TFLWSITPLF PRFLSEFKAG TFMGVASSIV 

      1030       1040       1050       1060       1070       1080 
SLFQNSRTIR NVFRDYMSQT IDDLIVKSEL TSLEHLSNYT DRKGSGGIWG CSAEQADKLR 

      1090       1100       1110       1120       1130       1140 
RMSWKRPVLG TTVPHPLEMH GRGTLKSPLS KCCKESRMDY ISVHIPEGLN KVLDGRGSLP 

      1150       1160       1170       1180       1190       1200 
AYLGSKTSES TSILQPWEKE SKIPIIRRAT RLRDAIHWFV DPDSNLARSI LNNIESLTGE 

      1210       1220       1230       1240       1250       1260 
KWEGALKGYK RTGSALHRFS TSRVSHGGFS SQSPACLTRM MATTDTMRDY AQLNYDFMFQ 

      1270       1280       1290       1300       1310       1320 
ASLLYSQMTS SVILMGTTVS NTIHFHVTCR KCIREITEPM LESPREYRGK DVHLVLAKWK 

      1330       1340       1350       1360       1370       1380 
NSSNGWGETL QLLKPVEGDW DTIPPVEKSY HVGRILGFLY GDLKSQNSSR ADDSSIFPLS 

      1390       1400       1410       1420       1430       1440 
IQMRLRGRGF LRGILDGLVR ASACQVIHRR SVALLSKPAN AIYGGLIYLI DKISASTSFT 

      1450       1460       1470       1480       1490       1500 
TLCRDGPIRE ELSSIPHKIP TSYPTSNSDM GLHIRNYLKF QCKTVELGKY QSDIKDLWLF 

      1510       1520       1530       1540       1550       1560 
SDVMTSNIAG PFALSTKILK CLYKPALSQK DRNNIRKISN FSKMMRSQLS WDPTSSEFIT 

      1570       1580       1590       1600       1610       1620 
SQILVCNEEI RHACKFGIPK LSLKFDDPVW GPEDYGLIWS IPVDYSSQSV PKNLKPCPRI 

      1630       1640       1650       1660       1670       1680 
QNPSISGFRL GQLPTGAHYK LRSILRKKNI HYRDALCGGD GSGGMTAAVL RYNLKARAIF 

      1690       1700       1710       1720       1730       1740 
NSILDFDGST MKGASPDPPS ALETVVNGRT RCVNAESCWE NPSDLSEQRT WDYFKFLKTH 

      1750       1760       1770       1780       1790       1800 
HGLKIDLIVL DMEVRDFAIS ASIEKCVRHN VSSILEEDGV LIYKTYGSTI AAESSNAVVN 

      1810       1820       1830       1840       1850       1860 
IGVLFESVEL IQTEYSSTST SEVYMYCRKI KKFVDAPHPD WISLDYYWSK LLCFRSYKEE 

      1870       1880       1890       1900       1910       1920 
FFRSYEVSRK ESLKGIPNSF IPDPLVNLET LLQIAGVPSG ISHQLAIDIK ESQLTQITAA 

      1930       1940       1950       1960       1970       1980 
MVLCGMIANY TLDVTKKRDS YNPPSDGRLI RMSAALVGIS FWISVKYYDK ELNFELEQIL 

      1990       2000       2010       2020       2030       2040 
SRSFPIRWML SHNYLFWTTK KGFRNAKDVR LSGNMANIGN WIRCMELLHL PPGSLSKDEV 

      2050       2060       2070       2080       2090 
TTTCGKYIRN LKYSVILQQT GIIDLWKSRV ASDDRSLMEV KTEFIESEHW VD

P13179 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools