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UniProtKB/Swiss-Prot entry P12576

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name L_MEASE
Primary accession number P12576
Secondary accession numbers None
Integrated into Swiss-Prot on October 1, 1989
Sequence was last modified on October 1, 1989 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 54)
Name and origin of the protein
Protein name Large structural protein
Synonyms Protein L
Transcriptase
Replicase
Includes RNA-directed RNA polymerase
     (EC 2.7.7.48)
mRNA (guanine-N(7)-)-methyltransferase
     (EC 2.1.1.56)
mRNA guanylyltransferase
     (EC 2.7.7.-)
Gene name
Name: L
From
Measles virus (strain Edmonston) (Subacute sclerose panencephalitis virus) [TaxID: 11235] 
Taxonomy Viruses; ssRNA negative-strand viruses; Mononegavirales; Paramyxoviridae; Paramyxovirinae; Morbillivirus.
Virus host Homo sapiens (Human) [TaxID: 9606]
Protein existence 2: Evidence at transcript level;
References
[1]
 NUCLEOTIDE SEQUENCE [MRNA].
DOI=10.1016/0042-6822(88)90563-6; PubMed=2835864 [NCBI, ExPASy, EBI, Israel, Japan]
Blumberg B.M., Crowley J.C., Silverman J.I., Menonna J., Cook S.D., Dowling P.C.;
"Measles virus L protein evidences elements of ancestral RNA polymerase.";
Virology 164:487-497(1988).
[2]
 NUCLEOTIDE SEQUENCE.
DOI=10.1016/0042-6822(89)90554-0; PubMed=2596022 [NCBI, ExPASy, EBI, Israel, Japan]
Cattaneo R., Schmid A., Spielhofer P., Kaelin K., Baczko K., Meulen V., Pardowitz J., Flanagan S., Rima B.K., Udem S.A.;
"Mutated and hypermutated genes of persistent measles viruses which caused lethal human brain diseases.";
Virology 173:415-425(1989).
Comments
  • FUNCTION: Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently the subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The transcriptase stutters on a specific sequence, resulting on a cotranscriptional editing of the phosphoprotein (P) mRNA. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals (By similarity).
  • CATALYTIC ACTIVITY: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
  • CATALYTIC ACTIVITY: S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
  • SUBUNIT: Interacts with the P protein (By similarity).
  • SUBCELLULAR LOCATION: Virion (Potential). Cytoplasm (By similarity).
  • SIMILARITY: Belongs to the paramyxoviruses L protein family.
  • SIMILARITY: Contains 1 RdRp catalytic domain.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
M20865; AAA46430.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
K01711; AAA75501.1; -; Genomic_RNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A28919; ZLNZMV.
3D structure databases
ModBase P12576.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from electronic annotation from UniProtKB-KW).
GO:0019012; Cellular component: virion (inferred from electronic annotation from UniProtKB-KW).
GO:0005524; Molecular function: ATP binding (inferred from electronic annotation from UniProtKB-KW).
GO:0004482; Molecular function: mRNA (guanine-N7-)-methyltransferase activity (inferred from electronic annotation from EC).
GO:0003723; Molecular function: RNA binding (inferred from electronic annotation from InterPro).
GO:0003968; Molecular function: RNA-directed RNA polymerase activity (inferred from electronic annotation from InterPro).
GO:0006370; Biological process: mRNA capping (inferred from electronic annotation from UniProtKB-KW).
GO:0006410; Biological process: transcription, RNA-dependent (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR016269; RNA-dir_RNA_pol_paramyxovir.
IPR014023; RNA_pol_cat.
IPR001016; RNA_pol_L_viral.
Graphical view of domain structure.
Pfam PF00946; Paramyx_RNA_pol; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF000830; RNA_pol_ParamyxoV; 1.
PROSITE PS50526; RDRP_SSRNA_NEG_NONSEG; 1.
PROSITE graphical view of domain structure (profiles).
ProtoNet P12576.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
ATP-binding; Cytoplasm; Methyltransferase; mRNA capping; mRNA processing; Multifunctional enzyme; Nucleotide-binding; Nucleotidyltransferase; RNA replication; RNA-directed RNA polymerase; S-adenosyl-L-methionine; Transferase; Virion.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom    To Length Description FTId
CHAIN   1   2183  2183     Large structural protein. PRO_0000142729
DOMAIN   656    840  185     RdRp catalytic. 
NP_BIND   1785   1794  10     ATP (Potential). 
Sequence information
Length: 2183 AA [This is the length of the unprocessed precursor] Molecular weight: 247648 Da [This is the MW of the unprocessed precursor] CRC64: 1B0B03CA2E2B6EA5 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MDSLSVNQIL YPEVHLDSPI VTNKIVAILE YARVPHAYSL EDPTLCQNIK HRLKNGFSNQ 

        70         80         90        100        110        120 
MIINNVEVGN VIKSKLRSYP AHSHIPYPNC NQDLFNIEDK ESTRKIRELL KKGNSLYSKV 

       130        140        150        160        170        180 
SDKVFQCLRD TNSRLGLGSE LREDIKEKVI NLGVYMHSSQ WFEPFLFWFT VKTEMRSVIK 

       190        200        210        220        230        240 
SQTHTCHRRR HTPVFFTGSS VELLISRDLV AIISKESQHV YYLTFELVLM YCDVIEGRLM 

       250        260        270        280        290        300 
TETAMTIDAR YTELLGRVRY MWKLIDGFFP ALGNPTYQIV AMLEPLSLAY LQLRDITVEL 

       310        320        330        340        350        360 
RGAFLNHCFT EIHDVLDQNG FSDEGTYHEL IEALDYIFIT DDIHLTGEIF SFFRSFGHPR 

       370        380        390        400        410        420 
LEAVTAAENV RKYMNQPKVI VYETLMKGHA IFCGIIINGY RDRHGGSWPP LTLPLHAADT 

       430        440        450        460        470        480 
IRNAQASGEG LTHEQCVDNW KSFAGVKFGC FMPLSLDSDL TMYLKDKALA ALQREWDSVY 

       490        500        510        520        530        540 
PKEFLRYDPP KGTGSRRLVD VFLNDSSFDP YDVIMYVVSG AYLHDPEFNL SYSLQEKEIK 

       550        560        570        580        590        600 
ETGRLFAKMT YKMRACQVIA ENLISNGIGK YFKDNGMAKD EQDLTKALHT LAVSGVPKDL 

       610        620        630        640        650        660 
KESHRGGPVL KTYSRSPVHT STRNVRAAKG FIGFPQVIRQ DQDTDHPENM EAYETVSAFI 

       670        680        690        700        710        720 
TTDLKKYCLN WRYETISLFA QRLNEIYGLP SFFQWLHKRL ETSVLYVSDP HCPPDLDAHI 

       730        740        750        760        770        780 
PLYKVPNDQI FIKYPMGGIE GYCQKLWTIS TIPYLYLAAY ESGVRIASLV QGDNQTIAVT 

       790        800        810        820        830        840 
KRVPSTWPYN LKKREAARVT RDYFVILRQR LHDIGHHLKA NETIVSSHFF VYSKGIYYDG 

       850        860        870        880        890        900 
LLVSQSLKSI ARCVFWSETI VDETRAACSN IATTMAKSIE RGYDRYLAYS LNFLKVIQQI 

       910        920        930        940        950        960 
LISLGFTINS TMTRDVVIPL LTNNDLLIRM ALLPAPIGGM NYLNMSRLFV RNIGDPVTSS 

       970        980        990       1000       1010       1020 
IADLKRMILA SLMPEETLHQ VMTQQPGDSS FLDWASDPYS ANLVCVQSIT RLLKNITARF 

      1030       1040       1050       1060       1070       1080 
VLIHSPNPML KGLFHDDSKE EDEGLAAFLM DRHIIVPRAA HEILDHSVTG ARESIAGMLD 

      1090       1100       1110       1120       1130       1140 
TTKGLIRASM RKGGLTSRVI TRLSNYDYEQ FRAGMVLLTG RKRNVLIDKE SCSVQLARAL 

      1150       1160       1170       1180       1190       1200 
RSHMWARLAR GRPIYGLEVP DVLESMRGHL IRRHETCVIC ECGSVNYGWF FVPSGCQLDD 

      1210       1220       1230       1240       1250       1260 
IDKETSSLRV PYIGSTTDER TDMKLAFVRA PSRSLRSAVR IATVYSWAYG DDDSSWNEAW 

      1270       1280       1290       1300       1310       1320 
LLARQRANVS LEELRVITPI STSTNLAHRL RDRSTQVKYS GTSLVRVARY TTISNDNLSF 

      1330       1340       1350       1360       1370       1380 
VISDKKVDTN FIYQQGMLLG LGVLETLFRL EKDTGSSNTV LHLHVETDCC VIPMIDHPRI 

      1390       1400       1410       1420       1430       1440 
PSSRKLELRA ELCTNPLIYD NAPLIDRDAT RLYTQSHRRH LVEFVTWSTP QLYHILAKST 

      1450       1460       1470       1480       1490       1500 
ALSMIDLVTK FEKDHMNEIS ALIGDDDINS FITEFLLIEP RLFTIYLGQC AAINWAFDVH 

      1510       1520       1530       1540       1550       1560 
YHRPSGKYQM GELLSSFLSR MSKGVFKVLV NALSHPKIYK KFWHCGIIEP IHGPSLDAQN 

      1570       1580       1590       1600       1610       1620 
LHTTVCNMVY TCYMTYLDLL LNEELEEFTF LLCESDEDVV PDRFDNIQAK HLCVLADLYC 

      1630       1640       1650       1660       1670       1680 
QPGTCPPIQG LRPVEKCAVL TDHIKAEAML SPAGSSWNIN PIIVDHYSCS LTYLRRGSIK 

      1690       1700       1710       1720       1730       1740 
QIRLRVDPGF IFDALAEVNV SQPKIGSNNI SNMSIKAFRP PHDDVAKLLK DINTSKHNLP 

      1750       1760       1770       1780       1790       1800 
ISGGNLANYE IHAFRRIGLN SSACYKAVEI STLIRRCLEP GEDGLFLGEG SGSMLITYKE 

      1810       1820       1830       1840       1850       1860 
ILKLSKCFYN SGVSANSRSG QRELAPYPSE VGLVEHRMGV GNIVKVLFNG RPEVTWVGSV 

      1870       1880       1890       1900       1910       1920 
DCFNFIVSNI PTSSVGFIHS DIETLPDKDT IEKLEELAAI LSMALLLGKI GSILVIKLMP 

      1930       1940       1950       1960       1970       1980 
FSGDFVQGFI SYVGSHYREV NLVYPRYSNF ISTESYLVMT DLKANRLMNP EKIKQQIIES 

      1990       2000       2010       2020       2030       2040 
SVRTSPGLIG HILSIKQLSC IQAIVGDAVS RGDINPTLKK LTPIEQVLIN CGLAINGPKL 

      2050       2060       2070       2080       2090       2100 
CKELIHHDVA SGQDGLLNSI LILYRELARF KDNQRSQQGM FHAYPVLVSS RQRELISRIT 

      2110       2120       2130       2140       2150       2160 
RKFWGHILLY SGNRKLINKF IQNLKSGYLI LDLHQNIFVK NLSKSEKQII MTGGLKREWV 

      2170       2180 
FKVTVKETKE WYKLVGYSAL IKD
P12576 in FASTA format

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