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UniProtKB/Swiss-Prot entry P11442

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name CLH_RAT
Primary accession number P11442
Secondary accession numbers None
Integrated into Swiss-Prot on October 1, 1989
Sequence was last modified on January 23, 2007 (Sequence version 3)
Annotations were last modified on    July 22, 2008 (Entry version 71)
Name and origin of the protein
Protein name Clathrin heavy chain 1
Synonyms None
Gene name
Name: Cltc
From
Rattus norvegicus (Rat) [TaxID: 10116] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [MRNA].
PubMed=3480512 [NCBI, ExPASy, EBI, Israel, Japan]
Kirchhausen T., Harrison S.C., Chow E.P., Mattaliano R.J., Ramachandran K.L., Smart J., Brosius J.;
"Clathrin heavy chain: molecular cloning and complete primary structure.";
Proc. Natl. Acad. Sci. U.S.A. 84:8805-8809(1987).
[2]
 PROTEIN SEQUENCE OF 2-8; 87-96; 177-205; 270-278; 355-382; 838-851; 882-892; 896-903; 1011-1034; 1095-1101; 1123-1130; 1216-1226; 1398-1406; 1435-1441; 1444-1453; 1502-1508; 1536-1545 AND 1610-1620, CLEAVAGE OF INITIATOR METHIONINE, ACETYLATION AT ALA-2, AND MASS SPECTROMETRY.
TISSUE=Pheochromocytoma;
Bienvenut W.V., von Kriegsheim A.F., Kolch W.;
Submitted (AUG-2006) to UniProtKB.
[3]
 X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 1-493.
DOI=10.1016/S0092-8674(00)81623-2; PubMed=9827808 [NCBI, ExPASy, EBI, Israel, Japan]
Ter Haar E., Musacchio A., Harrison S.C., Kirchhausen T.;
"Atomic structure of clathrin: a beta propeller terminal domain joins an alpha zigzag linker.";
Cell 95:563-573(1998).
Comments
  • FUNCTION: Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network.
  • SUBUNIT: Clathrin triskelions, composed of 3 heavy chains and 3 light chains, are the basic subunits of the clathrin coat. In the presence of light chains, hub assembly is influenced by both the pH and the concentration of calcium.
  • SUBCELLULAR LOCATION: Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Membrane, coated pit; Peripheral membrane protein; Cytoplasmic side. Melanosome (By similarity). Note=Cytoplasmic face of coated pits and vesicles.
  • PTM: The N-terminus is blocked.
  • SIMILARITY: Belongs to the clathrin heavy chain family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
J03583; AAA40874.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A39941; LRRTH.
RefSeq NP_062172.1; -.
UniGene Rn.3589
3D structure databases
PDB
1BPO; X-ray; 2.60 A; A/B/C=1-495.[ExPASy / RCSB / EBI]
1C9I; X-ray; 2.90 A; A/B=1-360.[ExPASy / RCSB / EBI]
1C9L; X-ray; 2.90 A; A/B=4-360.[ExPASy / RCSB / EBI]
Detailed list of linked structures.
PDBsum 1BPO; -.
1C9I; -.
1C9L; -.
ModBase P11442.
Protein-protein interaction databases
IntAct P11442; -.
PTM databases
PhosphoSite P11442; -.
Organism-specific databases
RGD 2364; Cltc.
Gene expression databases
ArrayExpress P11442; -.
GermOnline ENSRNOG00000004291; Rattus norvegicus.
Ontologies
GO
GO:0030669; Cellular component: clathrin-coated endocytic vesicle membrane (inferred from experiment from Reactome).
QuickGo view.
Family and domain databases
InterPro IPR000547; Clathrin_H-chain/VPS_repeat.
IPR016025; Clathrin_H-chain_link/propller.
IPR015348; Clathrin_H-chain_linker_core.
IPR001473; Clathrin_H-chain_propeller_N.
IPR016341; Clathrin_heavy_chain.
IPR011990; TPR-like_helical.
Graphical view of domain structure.
Gene3D G3DSA:2.130.10.110; Clathrin_H-chain_link/propller; 1.
G3DSA:1.25.40.10; TPR-like_helical; 1.
Pfam PF00637; Clathrin; 7.
PF09268; Clathrin-link; 1.
PF01394; Clathrin_propel; 7.
Pfam graphical view of domain structure.
PIRSF PIRSF002290; Clathrin_H_chain; 1.
SMART SM00299; CLH; 7.
SMART graphical view of domain structure.
BLOCKS P11442.
Genome annotation databases
Ensembl ENSRNOG00000004291; Rattus norvegicus. [Contig view]
GeneID 54241; -.
KEGG rno:54241; -.
Phylogenomic databases
HOVERGEN P11442; -.
Other
ProtoNet P11442.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Acetylation; Coated pit; Cytoplasmic vesicle; Direct protein sequencing; Membrane; Phosphoprotein.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom    To Length Description FTId
INIT_MET   1      1        Removed. 
CHAIN   2   1675  1674     Clathrin heavy chain 1. PRO_0000205780
REGION   2    479  478     Globular terminal domain. 
REGION   449    465  17     Binding site for the uncoating ATPase, involved in lattice disassembly (Potential). 
REGION   480    523  44     Flexible linker. 
REGION   524   1675  1152     Heavy chain arm. 
REGION   524    634  111     Distal segment. 
REGION   639   1675  1037     Proximal segment. 
REGION   1213   1522  310     Involved in binding clathrin light chain (By similarity). 
REGION   1550   1675  126     Trimerization (By similarity). 
MOD_RES   2      2        N-acetylalanine. 
MOD_RES   184    184        Phosphotyrosine (By similarity). 
MOD_RES   394    394        Phosphothreonine (By similarity). 
MOD_RES   634    634        Phosphotyrosine (By similarity). 
MOD_RES   899    899        Phosphotyrosine (By similarity). 
MOD_RES   1206   1206        Phosphotyrosine (By similarity). 
MOD_RES   1477   1477        Phosphotyrosine (By similarity). 
MOD_RES   1487   1487        Phosphotyrosine (By similarity). 
MOD_RES   1494   1494        Phosphoserine (By similarity). 
STRAND   7     14  8      
HELIX   15     18  4      
TURN   27     29  3      
STRAND   30     34  5      
STRAND   37     42  6      
STRAND   49     54  6      
STRAND   62     65  4      
STRAND   71     73  3      
STRAND   75     78  4      
STRAND   80     84  5      
STRAND   87     92  6      
TURN   93     96  4      
STRAND   97    103  7      
STRAND   110    115  6      
STRAND   118    122  5      
STRAND   124    135  12      
STRAND   139    143  5      
HELIX   146    148  3      
STRAND   152    158  7      
STRAND   162    173  12      
STRAND   176    186  11      
STRAND   191    194  4      
STRAND   197    205  9      
STRAND   210    220  11      
STRAND   227    232  6      
STRAND   246    249  4      
STRAND   261    267  7      
TURN   268    271  4      
STRAND   272    277  6      
STRAND   280    286  7      
TURN   287    289  3      
STRAND   292    297  6      
STRAND   303    309  7      
TURN   310    313  4      
STRAND   314    319  6      
STRAND   323    329  7      
TURN   331    333  3      
HELIX   334    340  7      
HELIX   345    354  10      
HELIX   361    373  13      
HELIX   377    386  10      
HELIX   388    390  3      
HELIX   395    401  7      
HELIX   413    424  12      
HELIX   429    441  13      
HELIX   445    454  10      
HELIX   461    470  10      
HELIX   473    482  10      
Sequence information
Length: 1675 AA [This is the length of the unprocessed precursor] Molecular weight: 191599 Da [This is the MW of the unprocessed precursor] CRC64: C10F54C7ED8C5A61 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MAQILPIRFQ EHLQLQNLGI NPANIGFSTL TMESDKFICI REKVGEQAQV VIIDMNDPSN 

        70         80         90        100        110        120 
PIRRPISADS AIMNPASKVI ALKAGKTLQI FNIEMKSKMK AHTMTDDVTF WKWISLNTVA 

       130        140        150        160        170        180 
LVTDNAVYHW SMEGESQPVK MFDRHSSLAG CQIINYRTDA KQKWLLLTGI SAQQNRVVGA 

       190        200        210        220        230        240 
MQLYSVDRKV SQPIEGHAAS FAQFKMEGNA EESTLFCFAV RGQAGGKLHI IEVGTPPTGN 

       250        260        270        280        290        300 
QPFPKKAVDV FFPPEAQNDF PVAMQISEKH DVVFLITKYG YIHLYDLETG TCIYMNRISG 

       310        320        330        340        350        360 
ETIFVTAPHE ATAGIIGVNR KGQVLSVCVE EENIIPYITN VLQNPDLALR MAVRNNLAGA 

       370        380        390        400        410        420 
EELFARKFNA LFAQGNYSEA AKVAANAPKG ILRTPDTIRR FQSVPAQPGQ TSPLLQYFGI 

       430        440        450        460        470        480 
LLDQGQLNKY ESLELCRPVL QQGRKQLLEK WLKEDKLECS EELGDLVKSV DPTLALSVYL 

       490        500        510        520        530        540 
RANVPNKVIQ CFAETGQVQK IVLYAKKVGY TPDWIFLLRN VMRISPDQGQ QFAQMLVQDE 

       550        560        570        580        590        600 
EPLADITQIV DVFMEYNLIQ QCTAFLLDAL KNNRPSEGPL QTRLLEMNLM HAPQVADAIL 

       610        620        630        640        650        660 
GNQMFTHYDR AHIAQLCEKA GLLQRALEHF TDLYDIKRAV VHTHLLNPEW LVNYFGSLSV 

       670        680        690        700        710        720 
EDSLECLRAM LSANIRQNLQ IWVQVASKYH EQLSTQSLIE LFESFKSFEG LFYFLGSIVN 

       730        740        750        760        770        780 
FSQDPDVHFK YIQAACKTGQ IKEVERICRE SNCYDPERVK NFLKEAKLTD QLPLIIVCDR 

       790        800        810        820        830        840 
FDFVHDLVLY LYRNSLQKYI EIYVQKVNPS RLPVVIGGLL DVDCSEDVIK NLILVVRGQF 

       850        860        870        880        890        900 
STDELVAEVE KRNRLKLLLP WLEARIHEGC EEPATHNALA KIYIDSNNNP ERFLRENPYY 

       910        920        930        940        950        960 
DSRVVGKYCE KRDPHLACVA YERGQCDLEL INVCNENSLF KSLSRYLVRR KDPELWGSVL 

       970        980        990       1000       1010       1020 
LESNPYRRPL IDQVVQTALS ETQDPEEVSV TVKAFMTADL PNELIELLEK IVLDNSVFSE 

      1030       1040       1050       1060       1070       1080 
HRNLQNLLIL TAIKADRTRV MEYINRLDNY DAPDIANIAI SNELFEEAFA IFRKFDVNTS 

      1090       1100       1110       1120       1130       1140 
AVQVLIEHIG NLDRAYEFAE RCNEPAVWSQ LAKAQLQKGM VKEAIDSYIK ADDPSSYMEV 

      1150       1160       1170       1180       1190       1200 
VQAANTSGNW EELVKYLQMA RKKARESYVE TELIFALAKT NRLAELEEFI NGPNNAHIQQ 

      1210       1220       1230       1240       1250       1260 
VGDRCYDEKM YDAAKLLYNN VSNFGRLAST LVHLGEYQAA VDGARKANST RTWKEVCFAC 

      1270       1280       1290       1300       1310       1320 
VDGKEFRLAQ MCGLHIVVHA DELEELINYY QDRGYFEELI TMLEAALGLE RAHMGMFTEL 

      1330       1340       1350       1360       1370       1380 
AILYSKFKPQ KMREHLELFW SRVNIPKVLR AAEQAHLWAE LVFLYDKYEE YDNAIITMMN 

      1390       1400       1410       1420       1430       1440 
HPTDAWKEGQ FKDIITKVAN VELYYKAIQF YLEFKPLLLN DLLMVLSPRL AHTRAVNYFS 

      1450       1460       1470       1480       1490       1500 
KVKQLPLVKP YLRSVQNHNN KSVNESLNNL FITEEDYQAL RTSIDAYDNF DNISLAQRLE 

      1510       1520       1530       1540       1550       1560 
KHELIEFRRI AAYLFKGNNR WKQSVELCKK DSLYKDAMQY ASESKDTELA EELLQWFLQE 

      1570       1580       1590       1600       1610       1620 
EKRECFGACL FTCYDLLRPD VVLETAWRHN IMDFAMPYFI QVMKEYLTKV DKLDASESLR 

      1630       1640       1650       1660       1670 
KEEEQATETQ PIVYGQPQLM LTAGPSVAVP PQAPFGYGYT APPYGQPQPG FGYSM
P11442 in FASTA format

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