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UniProtKB/Swiss-Prot entry P07825


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name SYPH_RAT
Primary accession number P07825
Secondary accession number Q499R3
Integrated into Swiss-Prot on August 1, 1988
Sequence was last modified on August 1, 1988 (Sequence version 1)
Annotations were last modified on    June 16, 2009 (Entry version 81)
Name and origin of the protein
Protein name Synaptophysin
Synonym Major synaptic vesicle protein p38
Gene name
Name: Syp
From
Rattus norvegicus (Rat) [TaxID: 10116] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi; Muroidea; Muridae; Murinae; Rattus.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA].
PubMed=3123215 [NCBI, ExPASy, EBI, Israel, Japan]
Leube R.E., Kaiser P., Seiter A., Zimbelmann R., Franke W.W., Rehm H., Knaus P., Prior P., Betz H., Reinke H., Beyreuther K., Wiedenmann B.;
"Synaptophysin: molecular organization and mRNA expression as determined from cloned cDNA.";
EMBO J. 6:3261-3268(1987).
[2]
NUCLEOTIDE SEQUENCE [MRNA].
DOI=10.1093/nar/15.22.9607; PubMed=3120152 [NCBI, ExPASy, EBI, Israel, Japan]
Suedhof T.C., Lottspeich F., Greengard P., Mehl E., Jahn R.;
"The cDNA and derived amino acid sequences for rat and human synaptophysin.";
Nucleic Acids Res. 15:9607-9607(1987).
[3]
NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
DOI=10.1126/science.3120313; PubMed=3120313 [NCBI, ExPASy, EBI, Israel, Japan]
Suedhof T.C., Lottspeich F., Greengard P., Mehl E., Jahn R.;
"A synaptic vesicle protein with a novel cytoplasmic domain and four transmembrane regions.";
Science 238:1142-1144(1987).
[4]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
DOI=10.1016/0378-1119(91)90127-W; PubMed=1902431 [NCBI, ExPASy, EBI, Israel, Japan]
Bargou R.C.E.F., Leube R.E.;
"The synaptophysin-encoding gene in rat and man is specifically transcribed in neuroendocrine cells.";
Gene 99:197-204(1991).
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Prostate;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[6]
NUCLEOTIDE SEQUENCE [MRNA] OF 13-307.
TISSUE=Brain;
DOI=10.1083/jcb.105.6.2447; PubMed=3121632 [NCBI, ExPASy, EBI, Israel, Japan]
Buckley K.M., Floor E., Kelly R.B.;
"Cloning and sequence analysis of cDNA encoding p38, a major synaptic vesicle protein.";
J. Cell Biol. 105:2447-2456(1987).
[7]
INTERACTION WITH SIAH1 AND SIAH2, AND DEGRADATION.
STRAIN=Sprague-Dawley;
DOI=10.1074/jbc.M107857200; PubMed=11786535 [NCBI, ExPASy, EBI, Israel, Japan]
Wheeler T.C., Chin L.-S., Li Y., Roudabush F.L., Li L.;
"Regulation of synaptophysin degradation by mammalian homologues of Seven in Absentia.";
J. Biol. Chem. 277:10273-10282(2002).
Comments
  • FUNCTION: Possibly involved in structural functions as organizing other membrane components or in targeting the vesicles to the plasma membrane.
  • SUBUNIT: Homohexamer or homotetramer.
  • SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Multi-pass membrane protein. Cell junction, synapse, synaptosome.
  • TISSUE SPECIFICITY: Characteristic of a type of small (30-80 nm) neurosecretory vesicles, including presynaptic vesicles, but also vesicles of various neuroendocrine cells of both neuronal and epithelial phenotype.
  • DOMAIN: The calcium-binding activity is thought to be localized in the cytoplasmic tail of the protein.
  • PTM: Ubiquitinated; mediated by SIAH1 or SIAH2 and leading to its subsequent proteasomal degradation.
  • SIMILARITY: Belongs to the synaptophysin/synaptobrevin family.
  • SIMILARITY: Contains 1 MARVEL domain.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X06177; CAA29543.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X06388; CAA29685.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M58396; AAA42196.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M58402; AAA42196.1; JOINED; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M58401; AAA42196.1; JOINED; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M58399; AAA42196.1; JOINED; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC099798; AAH99798.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
X06655; CAA29854.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
IPI IPI00211360; -.
PIR JU0464; B27287.
RefSeq NP_036796.1; -.
UniGene Rn.11067
3D structure databases
ModBase P07825.
Protein-protein interaction databases
IntAct P07825; 4.
PTM databases
PhosphoSite P07825; -.
Organism-specific databases
RGD 3802; Syp.
Gene expression databases
ArrayExpress P07825; -.
GermOnline ENSRNOG00000010223; Rattus norvegicus.
Ontologies
GO
GO:0030054; Cellular component: cell junction (inferred from electronic annotation from UniProtKB-SubCell).
GO:0030285; Cellular component: integral to synaptic vesicle membrane (traceable author statement from RGD).
GO:0043234; Cellular component: protein complex (inferred from direct assay from RGD).
GO:0030141; Cellular component: secretory granule (inferred from electronic annotation from UniProtKB-SubCell).
GO:0019717; Cellular component: synaptosome (inferred from direct assay from RGD).
GO:0005509; Molecular function: calcium ion binding (inferred from electronic annotation from UniProtKB-KW).
GO:0032403; Molecular function: protein complex binding (inferred from physical interaction from RGD).
GO:0017075; Molecular function: syntaxin-1 binding (inferred from direct assay from MGI).
GO:0005215; Molecular function: transporter activity (inferred from electronic annotation from InterPro).
GO:0048168; Biological process: regulation of neuronal synaptic plasticity (non-traceable author statement from RGD).
GO:0006810; Biological process: transport (inferred from electronic annotation from InterPro).
QuickGo view.
Family and domain databases
InterPro IPR008253; Marvel.
IPR001285; Synaptophysin/porin.
IPR015457; Synaptophysin_.
Graphical view of domain structure.
PANTHER PTHR10306; Synaptophysin; 1.
PTHR10306:SF2; Synaptophysin_; 1.
Pfam PF01284; MARVEL; 1.
Pfam graphical view of domain structure.
PRINTS PR00220; SYNAPTOPHYSN.
PROSITE PS51225; MARVEL; 1.
PS00604; SYNAPTOP; 1.
PROSITE graphical view of domain structure (profiles).
Proteomic databases
PRIDE P07825; -.
Genome annotation databases
Ensembl ENSRNOG00000010223; Rattus norvegicus. [Contig view]
GeneID 24804; -.
KEGG rno:24804; -.
Phylogenomic databases
HOVERGEN P07825; -.
Other
NextBio 604472; -.
ProtoNet P07825.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Calcium; Cell junction; Cytoplasmic vesicle; Direct protein sequencing; Glycoprotein; Membrane; Phosphoprotein; Repeat; Synapse; Synaptosome; Transmembrane; Ubl conjugation.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   307  307     Synaptophysin. PRO_0000179163
TOPO_DOM   1    19  19     Cytoplasmic (Potential). 
TRANSMEM   20    43  24     Potential. 
TOPO_DOM   44   101  58     Vesicular (Potential). 
TRANSMEM   102   125  24     Potential. 
TOPO_DOM   126   132  7     Cytoplasmic (Potential). 
TRANSMEM   133   156  24     Potential. 
TOPO_DOM   157   194  38     Vesicular (Potential). 
TRANSMEM   195   218  24     Potential. 
TOPO_DOM   219   307  89     Cytoplasmic (Potential). 
DOMAIN   15   222  208     MARVEL. 
REGION   249   298  50     Repeats, Gly-rich. 
MOD_RES   75    75        Phosphotyrosine (By similarity). 
MOD_RES   221   221        Phosphothreonine (Potential). 
MOD_RES   273   273        Phosphotyrosine (Potential). 
MOD_RES   289   289        Phosphotyrosine (Potential). 
CARBOHYD   53    53        N-linked (GlcNAc...) (Potential). 
Sequence information
Length: 307 AA [This is the length of the unprocessed precursor] Molecular weight: 33311 Da [This is the MW of the unprocessed precursor] CRC64: A245E4F70BF0EAF0 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MDVVNQLVAG GQFRVVKEPL GFVKVLQWVF AIFAFATCGS YTGELRLSVE CANKTESALN 

        70         80         90        100        110        120 
IEVEFEYPFR LHQVYFDAPS CVKGGTTKIF LVGDYSSSAE FFVTVAVFAF LYSMGALATY 

       130        140        150        160        170        180 
IFLQNKYREN NKGPMMDFLA TAVFAFMWLV SSSAWAKGLS DVKMATDPEN IIKEMPMCRQ 

       190        200        210        220        230        240 
TGNTCKELRD PVTSGLNTSV VFGFLNLVLW VGNLWFVFKE TGWAAPFMRA PPGAPEKQPA 

       250        260        270        280        290        300 
PGDAYGDAGY GQGPGGYGPQ DSYGPQGGYQ PDYGQPASGG GGYGPQGDYG QQGYGQQGAP 


TSFSNQM 

P07825 in FASTA format

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