[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. STRAIN=S203; Green R.L., Warren G.J.; "Physical and functional repetition in a bacterial ice nucleation gene."; Nature 317:645-648(1985).
[2]	SUBCELLULAR LOCATION. PubMed=2520825 [NCBI, ExPASy, EBI, Israel, Japan] Lindow S.E., Lahue E., Govindarajan A.G., Panopoulos N.J., Gies D.; "Localization of ice nucleation activity and the iceC gene product in Pseudomonas syringae and Escherichia coli."; Mol. Plant Microbe Interact. 2:262-272(1989).
[3]	3D-STRUCTURE MODELING OF 490-535. DOI=10.1006/jmbi.1993.1424; PubMed=8355267 [NCBI, ExPASy, EBI, Israel, Japan] Kajava A.V., Lindow S.E.; "A model of the three-dimensional structure of ice nucleation proteins."; J. Mol. Biol. 232:709-717(1993).
[4]	BIOTECHNOLOGICAL RELEVANCE. PubMed=1760850 [NCBI, ExPASy, EBI, Israel, Japan] Margaritis A., Bassi A.S.; "Principles and biotechnological applications of bacterial ice nucleation."; Crit. Rev. Biotechnol. 11:277-295(1991).

Comments

FUNCTION: Ice nucleation proteins enable bacteria to nucleate crystallization in supercooled water.
SUBCELLULAR LOCATION: Cell outer membrane.
DOMAIN: Contains 122 imperfect repeats of a consensus octapeptide A-G-Y-G-S-T-L-T; further on a 16-residue and a regional 48-residue periodicity is superimposed.
BIOTECHNOLOGY: Used by snowmaking industry to promote ice nucleation for artificial snow and economize water. Sold under the name Snomax by York.
MISCELLANEOUS: A structural model is suggested in which the ice nucleation protein displays a symmetry related to that of ice.
SIMILARITY: Belongs to the bacterial ice nucleation protein family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

X03035; CAA26837.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

A24405; SNPSO.

3D structure databases

PDB

1INA; Model; -; A=490-535.	[ExPASy / RCSB / EBI]
1INB; Model; -; A=490-535.	[ExPASy / RCSB / EBI]

Detailed list of linked structures.

PDBsum

1INA; -.
1INB; -.

ModBase

P06620.

Family and domain databases

InterPro

IPR000258; Ice_nucleatn.
Graphical view of domain structure.

Pfam

PF00818; Ice_nucleation; 61.
Pfam graphical view of domain structure.

PRINTS

PR00327; ICENUCLEATN.

PROSITE

PS00314; ICE_NUCLEATION; 40.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

3D-structure; Cell membrane; Cell outer membrane; Ice nucleation; Membrane; Repeat.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 1200`	`1200`	`Ice nucleation protein.`	`PRO_0000204024`
`REGION`	`176 1151`	`976`	`Octapeptide periodicity.`
`STRAND`	`492 495`	`4`
`STRAND`	`498 501`	`4`
`STRAND`	`503 505`	`3`
`STRAND`	`507 511`	`5`
`STRAND`	`514 518`	`5`
`STRAND`	`520 522`	`3`
`STRAND`	`524 527`	`4`
`STRAND`	`530 533`	`4`

Sequence information

Length: 1200 AA [This is the length of the unprocessed precursor]

Molecular weight: 118588 Da [This is the MW of the unprocessed precursor]

CRC64: E3BEAD4BEA67ABB0 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MNLDKALVLR TCANNMADHC GLIWPASGTV ESRYWQSTRR HENGLVGLLW GAGTSAFLSV 

        70         80         90        100        110        120 
HADARWIVCE VAVADIISLE EPGMVKFPRA EVVHVGDRIS ASHFISARQA DPASTSTSTL 

       130        140        150        160        170        180 
TPMPTAIPTP MPAVASVTLP VAEQARHEVF DVASVSAAAA PVNTLPVTTP QNVQTATYGS 

       190        200        210        220        230        240 
TLSGDNHSRL IAGYGSNETA GNHSDLIAGY GSTGTAGSDS WLVAGYGSTQ TAGGDSALTA 

       250        260        270        280        290        300 
GYGSTQTARE GSNLTAGYGS TGTAGSDSSL IAGYGSTQTS GGDSSLTAGY GSTQTAQEGS 

       310        320        330        340        350        360 
NLTAGYGSTG TAGSDSSLIA GYGSTQTSGG DSSLTAGYGS TQTAQEGSNL TAGYGSTGTA 

       370        380        390        400        410        420 
GVDSSLIAGY GSTQTSGSDS ALTAGYGSTQ TAQEGSNLTA GYGSTGTAGS DSSLIAGYGS 

       430        440        450        460        470        480 
TQTSGSDSSL TAGYGSTQTA QEGSILTAGY GSTGTAGVDS SLIAGYGSTQ TSGSDSALTA 

       490        500        510        520        530        540 
GYGSTQTAQE GSNLTAGYGS TGTAGADSSL IAGYGSTQTS GSESSLTAGY GSTQTAREGS 

       550        560        570        580        590        600 
TLTAGYGSTG TAGADSSLIA GYGSTQTSGS ESSLTAGYGS TQTAQQGSVL TSGYGSTQTA 

       610        620        630        640        650        660 
GAASNLTTGY GSTGTAGHES FIIAGYGSTQ TAGHKSILTA GYGSTQTARD GSDLIAGYGS 

       670        680        690        700        710        720 
TGTAGSGSSL IAGYGSTQTA SYRSMLTAGY GSTQTAREHS DLVTGYGSTS TAGSNSSLIA 

       730        740        750        760        770        780 
GYGSTQTAGF KSILTAGYGS TQTAQERTSL VAGYGSTSTA GYSSSLIAGY GSTQTAGYES 

       790        800        810        820        830        840 
TLTAGYGSTQ TAQENSSLTT GYGSTSTAGY SSSLIAGYGS TQTAGYESTL TAGYGSTQTA 

       850        860        870        880        890        900 
QERSDLVTGY GSTSTAGYAS SLIAGYGSTQ TAGYESTLTA GYGSTQTAQE NSSLTTGYGS 

       910        920        930        940        950        960 
TSTAGFASSL ISGYGSTQTA GYKSTLTAGY GSTQTAEYGS SLTAGYGSTA TAGQDSSLIA 

       970        980        990       1000       1010       1020 
GYGSSLTSGI RSFLTAGYGS TLIAGLRSVL IAGYGSSLTS GVRSTLTAGY GSNQIASYGS 

      1030       1040       1050       1060       1070       1080 
SLIAGHESIQ VAGNKSMLIA GKGSSQTAGF RSTLIAGAGS VQLAGDRSRL IAGADSNQTA 

      1090       1100       1110       1120       1130       1140 
GDRSKLLAGN NSYLTAGDRS KLTGGHDCTL MAGDQSRLTA GKNSVLTAGA RSKLIGSEGS 

      1150       1160       1170       1180       1190       1200 
TLSAGEDSIL IFRLWDGKRY RQLVARTGEN GVEADIPYYV NEDDDIVDKP DEDDDWIEVK

P06620 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools