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[1]
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NUCLEOTIDE SEQUENCE [GENOMIC RNA].
DOI=10.1038/313277a0; PubMed=2578615 [NCBI, ExPASy, EBI, Israel, Japan]
Ratner L.,
Haseltine W.A.,
Patarca R.,
Livak K.J.,
Starcich B.R.,
Josephs S.F.,
Doran E.R.,
Rafalski J.A.,
Whitehorn E.A.,
Baumeister K.,
Ivanoff L.,
Petteway S.R. Jr.,
Pearson M.L.,
Lautenberger J.A.,
Papas T.S.,
Ghrayeb J.,
Chang N.T.,
Gallo R.C.,
Wong-Staal F.;
"Complete nucleotide sequence of the AIDS virus, HTLV-III.";
Nature 313:277-284(1985).
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[2]
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REVIEW.
DOI=10.1016/S0005-2736(03)00163-9; PubMed=12873766 [NCBI, ExPASy, EBI, Israel, Japan]
Scarlata S.,
Carter C.;
"Role of HIV-1 Gag domains in viral assembly.";
Biochim. Biophys. Acta 1614:62-72(2003).
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- FUNCTION: Matrix protein p17 targets Gag and Gag-Pol polyproteins to the plasma membrane via a multipartite membrane binding signal, that includes its myristoylated N-terminus. Also mediates nuclear localization of the preintegration complex. Implicated in the release from host cell mediated by Vpu.
- FUNCTION: Capsid protein p24 forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.
- FUNCTION: Nucleocapsid protein p7 encapsulates and protects viral dimeric unspliced (genomic) RNA. Binds these RNAs through its zinc fingers.
- FUNCTION: p6-gag plays a role in budding of the assembled particle by interacting with the host class E VPS proteins TSG101 and PDCD6IP/AIP1 (By similarity).
- SUBUNIT: Matrix protein p17 is a trimer. Interacts with gp120. p6-gag interacts with human TSG101 and PDCD6IP/AIP1 (By similarity).
- SUBCELLULAR LOCATION: Matrix protein p17: Virion (Potential). Host nucleus (By similarity). Host cytoplasm (By similarity). Host cell membrane; Lipid-anchor (Potential). Note=Following virus entry, the nuclear localization signal (NLS) of the matrix protein participates with Vpr to the nuclear localization of the viral genome. During virus production, the nuclear export activity of the matrix protein counteracts the NLS to maintain the Gag and Gag-Pol polyproteins in the cytoplasm, thereby directing unspliced RNA to the plasma membrane (By similarity).
- SUBCELLULAR LOCATION: Capsid protein p24: Virion (Potential).
- SUBCELLULAR LOCATION: Nucleocapsid protein p7: Virion (Potential).
- ALTERNATIVE PRODUCTS:
2 named isoforms [FASTA] produced by ribosomal frameshifting. Translation results in the formation of the Gag polyprotein most of the time. Ribosomal frameshifting at the gag-pol genes boundary occurs at low frequency and produces the Gag-Pol polyprotein. This strategy of translation probably allows the virus to modulate the quantity of each viral protein. Maintenance of a correct Gag to Gag-Pol ratio is essential for RNA dimerization and viral infectivity.
| Name | Gag polyprotein |
| Isoform ID | P04593-1 |
| Note: Produced by conventional translation. |
| This is the isoform sequence displayed in this entry. |
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| Name | Gag-Pol polyprotein |
| Isoform ID | P04587-1 |
| Note: Produced by -1 ribosomal frameshifting. |
| This isoform is stored in UniProtKB/Swiss-Prot entry P04587. |
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- DOMAIN: Late-budding domains (L domains) are short sequence motifs essential for viral particle release. They can occur individually or in close proximity within structural proteins. They interacts with sorting cellular proteins of the multivesicular body (MVB) pathway. Most of these proteins are class E vacuolar protein sorting factors belonging to ESCRT-I, ESCRT-II or ESCRT-III complexes. p6-gag contains two L domains: a PTAP/PSAP motif, which interacts with the UEV domain of TSG101 and a LXXLF motif which interacts with PDCD6IP/AIP1 (By similarity).
- PTM: Capsid protein p24 is phosphorylated.
- PTM: Specific enzymatic cleavages by the viral protease yield mature proteins. The polyprotein is cleaved during and after budding, this process is termed maturation (By similarity).
- PTM: Nucleocapsid protein p7 is methylated by host PRMT6, impairing its function by reducing RNA annealing and the initiation of reverse transcription (By similarity).
- MISCELLANEOUS: HIV-1 lineages are divided in three main groups, M (for Major), O (for Outlier), and N (for New, or Non-M, Non-O). The vast majority of strains found worldwide belong to the group M. Group O seems to be endemic to and largely confined to Cameroon and neighboring countries in West Central Africa, where these viruses represent a small minority of HIV-1 strains. The group N is represented by a limited number of isolates from Cameroonian persons. The group M is further subdivided in 9 clades or subtypes (A to D, F to H, J and K).
- SIMILARITY: Belongs to the primate lentivirus group gag polyprotein family.
- SIMILARITY: Contains 2 CCHC-type zinc fingers.
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Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms.
Distributed under the Creative Commons Attribution-NoDerivs License.
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| Length: 512 AA [This is the length of the unprocessed precursor] |
Molecular weight: 57238 Da [This is the MW of the unprocessed precursor] |
CRC64: 243A82D3CBBE7A9C [This is a checksum on the sequence] |
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10 20 30 40 50 60
MGARASVLSG GELDRWEKIR LRPGGKKKYK LKHIVWASRE LERFAVNPGL LETSEGCRQI
70 80 90 100 110 120
LGQLQPSLQT GSEELRSLYN TVATLYCVHQ RIEIKDTKEA LDKIEEEQNK SKKKAQQAAA
130 140 150 160 170 180
DTGHSSQVSQ NYPIVQNIQG QMVHQAISPR TLNAWVKVVE EKAFSPEVIP MFSALSEGAT
190 200 210 220 230 240
PQDLNTMLNT VGGHQAAMQM LKETINEEAA EWDRVHPVHA GPIAPGQMRE PRGSDIAGTT
250 260 270 280 290 300
STLQEQIGWM TNNPPIPVGE IYKRWIILGL NKIVRMYSPT SILDIRQGPK EPFRDYVDRF
310 320 330 340 350 360
YKTLRAEQAS QEVKNWMTET LLVQNANPDC KTILKALGPA ATLEEMMTAC QGVGGPGHKA
370 380 390 400 410 420
RVLAEAMSQV TNSTTIMMQR GNFRNQRKIV KCFNCGKEGH IARNCKAPRK KGCWKCGKEG
430 440 450 460 470 480
HQMKDCTERQ ANFLGKIWPS YKGRPGNFLQ SRPEPTAPPF LQSRPEPTAP PEESFRSGVE
490 500 510
TTTPPQKQEP IDKELYPLTS LRSLFGNDPS SQ
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P04593 in FASTA format |
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