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UniProtKB/Swiss-Prot entry P02526


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name CRGB_BOVIN
Primary accession number P02526
Secondary accession number A2TJU8
Integrated into Swiss-Prot on July 21, 1986
Sequence was last modified on January 23, 2007 (Sequence version 2)
Annotations were last modified on    November 4, 2008 (Entry version 87)
Name and origin of the protein
Protein name Gamma-crystallin B
Synonyms Gamma-B-crystallin
Gamma-crystallin II
Gene name
Name: CRYGB
From
Bos taurus (Bovine) [TaxID: 9913] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia; Pecora; Bovidae; Bovinae; Bos.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA].
DOI=10.1016/0006-291X(87)90729-7; PubMed=3606621 [NCBI, ExPASy, EBI, Israel, Japan]
Hay R.E., Woods W.D., Church R.L., Petrash J.M.;
"cDNA clones encoding bovine gamma-crystallins.";
Biochem. Biophys. Res. Commun. 146:332-338(1987).
[2]
NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Lens;
PubMed=6092016 [NCBI, ExPASy, EBI, Israel, Japan]
Bhat S.P., Spector A.;
"Complete nucleotide sequence of a cDNA derived from calf lens gamma-crystallin mRNA: presence of Alu I-like DNA sequences.";
DNA 3:287-295(1984).
[3]
NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Lens;
Wistow G.;
Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases.
[4]
PROTEIN SEQUENCE OF 2-26.
DOI=10.1007/BF01025415; PubMed=3255364 [NCBI, ExPASy, EBI, Israel, Japan]
Chiou S.H., Azari P., Himmel M.E.;
"Physicochemical characterization of gamma-crystallins from bovine lens -- hydrodynamic and biochemical properties.";
J. Protein Chem. 7:67-80(1988).
[5]
PROTEIN SEQUENCE OF 2-26, AND DISULFIDE BOND.
DOI=10.1016/0003-9861(88)90413-4; PubMed=3364984 [NCBI, ExPASy, EBI, Israel, Japan]
McDermott M.J., Gawinowicz-Kolks M.A., Chiesa R., Spector A.;
"The disulfide content of calf gamma-crystallin.";
Arch. Biochem. Biophys. 262:609-619(1988).
[6]
GLYCATION AT LYS-3, AND MASS SPECTROMETRY.
DOI=10.1006/abio.1996.0501; PubMed=8954545 [NCBI, ExPASy, EBI, Israel, Japan]
Smith J.B., Hanson S.R., Cerny R.L., Zhao H.R., Abraham E.C.;
"Identification of the glycation site of lens gamma B-crystallin by fast atom bombardment tandem mass spectrometry.";
Anal. Biochem. 243:186-189(1996).
[7]
X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS), AND SEQUENCE REVISION.
DOI=10.1038/289771a0; PubMed=7464942 [NCBI, ExPASy, EBI, Israel, Japan]
Blundell T.L., Lindley P., Miller L., Moss D., Slingsby C., Tickle I., Turnell B., Wistow G.;
"The molecular structure and stability of the eye lens: X-ray analysis of gamma-crystallin II.";
Nature 289:771-777(1981).
[8]
X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS).
DOI=10.1016/S0022-2836(83)80232-0; PubMed=6631960 [NCBI, ExPASy, EBI, Israel, Japan]
Wistow G., Turnell B., Summers L., Slingsby C., Moss D., Miller L., Lindley P., Blundell T.L.;
"X-ray analysis of the eye lens protein gamma-II crystallin at 1.9-A resolution.";
J. Mol. Biol. 170:175-202(1983).
[9]
X-RAY CRYSTALLOGRAPHY (1.47 ANGSTROMS).
DOI=10.1107/S0907444992007601; PubMed=15299528 [NCBI, ExPASy, EBI, Israel, Japan]
Najmudin S., Nalini V., Dreissen H.P.C., Slingsby C., Blundell T.L., Moss D.S., Lindley P.F.;
"Structure of the bovine eye lens protein gammaB (gammaII)-crystallin at 1.47 A.";
Acta Crystallogr. D 49:223-233(1993).
[10]
X-RAY CRYSTALLOGRAPHY (1.2 ANGSTROMS).
DOI=10.1107/S0907444995014302; PubMed=15299624 [NCBI, ExPASy, EBI, Israel, Japan]
Kumaraswamy V.S., Lindley P.F., Slingsby C., Glover I.D.;
"An eye lens protein-water structure: 1.2-A resolution structure of gammaB-crystallin at 150 K.";
Acta Crystallogr. D 52:611-622(1996).
[11]
X-RAY CRYSTALLOGRAPHY (1.85 ANGSTROMS).
PubMed=9541393 [NCBI, ExPASy, EBI, Israel, Japan]
Palme S., Jaenicke R., Slingsby C.;
"X-ray structures of three interface mutants of gammaB-crystallin from bovine eye lens.";
Protein Sci. 7:611-618(1998).
[12]
X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF MUTANT ALA-57.
DOI=10.1006/jmbi.1998.1850; PubMed=9642083 [NCBI, ExPASy, EBI, Israel, Japan]
Palme S., Jaenicke R., Slingsby C.;
"Unusual domain pairing in a mutant of bovine lens gammaB-crystallin.";
J. Mol. Biol. 279:1053-1059(1998).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
X01036; CAA25518.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
M16894; AAA30476.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
EF208024; ABM97504.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A29655; CYBOG.
RefSeq NP_001013612.1; -.
UniGene Bt.399
3D structure databases
PDB
1AMM; X-ray; 1.20 A; A=1-175.[ExPASy / RCSB / EBI]
1DSL; X-ray; 1.55 A; A=88-175.[ExPASy / RCSB / EBI]
1GAM; X-ray; 2.60 A; A/B=88-173.[ExPASy / RCSB / EBI]
1GCS; X-ray; 2.00 A; A=1-175.[ExPASy / RCSB / EBI]
1I5I; X-ray; 2.40 A; A=1-175.[ExPASy / RCSB / EBI]
4GCR; X-ray; 1.47 A; A=1-175.[ExPASy / RCSB / EBI]
Detailed list of linked structures.
PDBsum 1AMM; -.
1DSL; -.
1GAM; -.
1GCS; -.
1I5I; -.
4GCR; -.
ModBase P02526.
Ontologies
GO
GO:0005212; Molecular function: structural constituent of eye lens (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR001064; Crystallin.
Graphical view of domain structure.
Gene3D G3DSA:2.60.20.10; Crystallin; 2.
Pfam PF00030; Crystall; 2.
Pfam graphical view of domain structure.
PRINTS PR01367; BGCRYSTALLIN.
SMART SM00247; XTALbg; 2.
SMART graphical view of domain structure.
PROSITE PS50915; CRYSTALLIN_BETA_GAMMA; 4.
PROSITE graphical view of domain structure (profiles).
ProtoNet P02526.
Genome annotation databases
Ensembl ENSBTAG00000021770; Bos taurus. [Contig view]
GeneID 281720; -.
KEGG bta:281720; -.
Phylogenomic databases
HOVERGEN P02526; -.
Other
LinkHub P02526; -.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Direct protein sequencing; Eye lens protein; Glycation; Glycoprotein; Oxidation; Phosphoprotein; Repeat.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
INIT_MET   1     1        Removed. 
CHAIN   2   175  174     Gamma-crystallin B. PRO_0000057581
DOMAIN   2    40  39     Beta/gamma crystallin 'Greek key' 1. 
DOMAIN   41    83  43     Beta/gamma crystallin 'Greek key' 2. 
DOMAIN   89   129  41     Beta/gamma crystallin 'Greek key' 3. 
DOMAIN   130   172  43     Beta/gamma crystallin 'Greek key' 4. 
REGION   84    88  5     Connecting peptide. 
SITE   69    69  1     Susceptible to oxidation (By similarity). 
SITE   70    70  1     Susceptible to oxidation (By similarity). 
MOD_RES   63    63        Phosphotyrosine (By similarity). 
MOD_RES   66    66        Phosphotyrosine (By similarity). 
CARBOHYD   3     3        N-linked (Glc) (glycation); in vitro. 
DISULFID   19    23         
CONFLICT   120   120        T -> S (in Ref. 2; CAA25518). 
STRAND   3     9  7      
HELIX   10    12  3      
STRAND   13    19  7      
TURN   27    29  3      
STRAND   34    41  8      
STRAND   43    48  6      
TURN   49    51  3      
STRAND   52    58  7      
STRAND   60    65  6      
HELIX   66    69  4      
STRAND   72    74  3      
STRAND   78    81  4      
STRAND   90    96  7      
TURN   97    99  3      
STRAND   100   106  7      
HELIX   113   117  5      
STRAND   124   130  7      
STRAND   132   137  6      
TURN   138   140  3      
STRAND   141   147  7      
STRAND   149   152  4      
HELIX   155   158  4      
STRAND   167   170  4      
Sequence information
Length: 175 AA [This is the length of the unprocessed precursor] Molecular weight: 21097 Da [This is the MW of the unprocessed precursor] CRC64: 0CF283DA837CC593 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MGKITFYEDR GFQGHCYECS SDCPNLQPYF SRCNSIRVDS GCWMLYERPN YQGHQYFLRR 

        70         80         90        100        110        120 
GDYPDYQQWM GFNDSIRSCR LIPQHTGTFR MRIYERDDFR GQMSEITDDC PSLQDRFHLT 

       130        140        150        160        170 
EVHSLNVLEG SWVLYEMPSY RGRQYLLRPG EYRRYLDWGA MNAKVGSLRR VMDFY 

P02526 in FASTA format

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