ID   NEU1_RAT                Reviewed;         125 AA.
AC   P01179; Q53WU2;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 2.
DT   25-NOV-2008, entry version 76.
DE   RecName: Full=Oxytocin-neurophysin 1;
DE            Short=OT-NPI;
DE   Contains:
DE     RecName: Full=Oxytocin;
DE     AltName: Full=Ocytocin;
DE   Contains:
DE     RecName: Full=Neurophysin 1;
DE   Flags: Precursor;
GN   Name=Oxt; Synonyms=Ot;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Sciurognathi;
OC   Muroidea; Muridae; Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=84193944; PubMed=6326097;
RA   Ivell R., Richter D.;
RT   "Structure and comparison of the oxytocin and vasopressin genes from
RT   rat.";
RL   Proc. Natl. Acad. Sci. U.S.A. 81:2006-2010(1984).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   MEDLINE=87026235; PubMed=3768139;
RA   Rehbein M., Hillers M., Mohr E., Ivell R., Morley S., Schmale H.,
RA   Richter D.;
RT   "The neurohypophyseal hormones vasopressin and oxytocin. Precursor
RT   structure, synthesis and regulation.";
RL   Biol. Chem. Hoppe-Seyler 367:695-704(1986).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   MEDLINE=89078601; PubMed=3203740; DOI=10.1016/0014-5793(88)81003-2;
RA   Mohr E., Bahnsen U., Kiessling C., Richter D.;
RT   "Expression of the vasopressin and oxytocin genes in rats occurs in
RT   mutually exclusive sets of hypothalamic neurons.";
RL   FEBS Lett. 242:144-148(1988).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Wistar; TISSUE=Liver;
RX   MEDLINE=91166731; PubMed=1706187;
RA   Schmitz E., Mohr E., Richter D.;
RT   "Rat vasopressin and oxytocin genes are linked by a long interspersed
RT   repeated DNA element (LINE): sequence and transcriptional analysis of
RT   LINE.";
RL   DNA Cell Biol. 10:81-91(1991).
RN   [5]
RP   PROTEIN SEQUENCE OF 32-125.
RX   MEDLINE=82134797; PubMed=7036996; DOI=10.1016/0006-291X(81)90493-9;
RA   Chauvet M.-T., Chauvet J., Acher R.;
RT   "Identification of rat neurophysins: complete amino acid sequences of
RT   MSEL- and VLDV-neurophysins.";
RL   Biochem. Biophys. Res. Commun. 103:595-603(1981).
RN   [6]
RP   PROTEIN SEQUENCE OF 32-100.
RX   MEDLINE=81212775; PubMed=7238864; DOI=10.1016/0014-5793(81)81028-9;
RA   North W.G., Mitchell T.I.;
RT   "Evolution of neurophysin proteins: partial amino acid sequences of
RT   rat neurophysins.";
RL   FEBS Lett. 126:41-44(1981).
RN   [7]
RP   PROTEIN SEQUENCE OF 32-64.
RX   MEDLINE=77246828; PubMed=891987; DOI=10.1016/0014-5793(77)80478-X;
RA   Schlesinger D.H., Pickering B.T., Watkins W.B., Peek J.C., Moore L.G.,
RA   Audhya T.K., Walter R.;
RT   "A comparative study of partial neurophysin protein sequences of cod,
RT   guinea pig, rat and sheep.";
RL   FEBS Lett. 80:371-373(1977).
RN   [8]
RP   PROTEIN SEQUENCE OF 20-28.
RX   MEDLINE=72215060; PubMed=5150741;
RA   Chauvet J., Chauvet M.-T., Acher R.;
RT   "Evolution of neurohypophyseal hormones: isolation of active
RT   principles from rabbits and rats.";
RL   Biochimie 53:1099-1104(1971).
CC   -!- FUNCTION: Neurophysin 1 specifically binds oxytocin.
CC   -!- FUNCTION: Oxytocin causes contraction of the smooth muscle of the
CC       uterus and of the mammary gland.
CC   -!- SIMILARITY: Belongs to the vasopressin/oxytocin family.
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DR   EMBL; K01701; AAA98733.1; -; Genomic_DNA.
DR   EMBL; M25649; AAA41773.1; -; mRNA.
DR   EMBL; X12792; CAA31281.1; -; Genomic_DNA.
DR   EMBL; X59496; CAA42085.1; -; Genomic_DNA.
DR   PIR; A21183; NFRT1.
DR   RefSeq; NP_037128.1; -.
DR   UniGene; Rn.48915; -.
DR   Ensembl; ENSRNOG00000021225; Rattus norvegicus.
DR   GeneID; 25504; -.
DR   KEGG; rno:25504; -.
DR   RGD; 3238; Oxt.
DR   HOVERGEN; P01179; -.
DR   LinkHub; P01179; -.
DR   NextBio; 606917; -.
DR   ArrayExpress; P01179; -.
DR   GermOnline; ENSRNOG00000021225; Rattus norvegicus.
DR   GO; GO:0005576; C:extracellular region; IEA:InterPro.
DR   GO; GO:0005185; F:neurohypophyseal hormone activity; IEA:InterPro.
DR   InterPro; IPR000981; Neurhyp_horm.
DR   InterPro; IPR016321; Nonapeptide_hormone_prcur.
DR   Gene3D; G3DSA:2.60.9.10; Neurhyp_horm; 1.
DR   Pfam; PF00220; Hormone_4; 1.
DR   Pfam; PF00184; Hormone_5; 1.
DR   PIRSF; PIRSF001815; Nonapeptide_hormone_precursor; 1.
DR   PRINTS; PR00831; NEUROPHYSIN.
DR   ProDom; PD001676; Neurhyp_horm; 1.
DR   SMART; SM00003; NH; 1.
DR   PROSITE; PS00264; NEUROHYPOPHYS_HORM; 1.
PE   1: Evidence at protein level;
KW   Amidation; Cleavage on pair of basic residues;
KW   Direct protein sequencing; Hormone; Signal.
FT   SIGNAL        1     19
FT   PEPTIDE      20     28       Oxytocin.
FT                                /FTId=PRO_0000020503.
FT   CHAIN        32    125       Neurophysin 1.
FT                                /FTId=PRO_0000020504.
FT   MOD_RES      28     28       Glycine amide.
FT   DISULFID     20     25
FT   DISULFID     41     85       By similarity.
FT   DISULFID     44     58       By similarity.
FT   DISULFID     52     75       By similarity.
FT   DISULFID     59     65       By similarity.
FT   DISULFID     92    104       By similarity.
FT   DISULFID     98    116       By similarity.
FT   DISULFID    105    110       By similarity.
FT   CONFLICT    100    100       T -> A (in Ref. 6; AA sequence).
FT   CONFLICT    124    125       ER -> Q (in Ref. 5; AA sequence).
SQ   SEQUENCE   125 AA;  12823 MW;  DC98F90BADC5551F CRC64;
     MACPSLACCL LGLLALTSAC YIQNCPLGGK RAALDLDMRK CLPCGPGGKG RCFGPSICCA
     DELGCFVGTA EALRCQEENY LPSPCQSGQK PCGSGGRCAT AGICCSPDGC RTDPACDPES
     AFSER
//