[1]	NUCLEOTIDE SEQUENCE [MRNA]. TISSUE=Submandibular gland; DOI=10.1038/302538a0; PubMed=6336309 [NCBI, ExPASy, EBI, Israel, Japan] Scott J., Selby M.J., Urdea M.S., Quiroga M., Bell G.I., Rutter W.J.; "Isolation and nucleotide sequence of a cDNA encoding the precursor of mouse nerve growth factor."; Nature 302:538-540(1983).
[2]	NUCLEOTIDE SEQUENCE [MRNA]. DOI=10.1038/303821a0; PubMed=6688123 [NCBI, ExPASy, EBI, Israel, Japan] Ullrich A., Gray A., Berman C., Dull T.J.; "Human beta-nerve growth factor gene sequence highly homologous to that of mouse."; Nature 303:821-825(1983).
[3]	NUCLEOTIDE SEQUENCE [MRNA]. PubMed=6327169 [NCBI, ExPASy, EBI, Israel, Japan] Ullrich A., Gray A., Berman C., Coussens L., Dull T.J.; "Sequence homology of human and mouse beta-NGF subunit genes."; Cold Spring Harb. Symp. Quant. Biol. 48:435-442(1983).
[4]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. STRAIN=C57BL/6; TISSUE=Submandibular gland; PubMed=3670305 [NCBI, ExPASy, EBI, Israel, Japan] Selby M.J., Edwards R., Sharp F., Rutter W.J.; "Mouse nerve growth factor gene: structure and expression."; Mol. Cell. Biol. 7:3057-3064(1987).
[5]	NUCLEOTIDE SEQUENCE [MRNA]. TISSUE=Skeletal muscle; DOI=10.1016/0197-0186(92)90155-K; PubMed=1284621 [NCBI, ExPASy, EBI, Israel, Japan] Yamamoto T., Yamakuni T., Okabe N., Amano T.; "Production and secretion of nerve growth factor by clonal striated muscle cell line, G8-1."; Neurochem. Int. 21:251-258(1992).
[6]	PROTEIN SEQUENCE OF 122-239. DOI=10.1021/bi00725a018; PubMed=4566923 [NCBI, ExPASy, EBI, Israel, Japan] Angeletti R.H., Hermodson M.A., Bradshaw R.A.; "Amino acid sequences of mouse 2.5S nerve growth factor. II. Isolation and characterization of the thermolytic and peptic peptides and the complete covalent structure."; Biochemistry 12:100-115(1973).
[7]	X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS). DOI=10.1038/354411a0; PubMed=1956407 [NCBI, ExPASy, EBI, Israel, Japan] McDonald N.Q., Lapatto R., Murray-Rust J., Gunning J., Wlodawer A., Blundell T.L.; "New protein fold revealed by a 2.3-A resolution crystal structure of nerve growth factor."; Nature 354:411-414(1991).
[8]	X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS). DOI=10.1006/jmbi.1994.1380; PubMed=8201620 [NCBI, ExPASy, EBI, Israel, Japan] Holland D.R., Cousens L.S., Meng W., Matthews B.W.; "Nerve growth factor in different crystal forms displays structural flexibility and reveals zinc binding sites."; J. Mol. Biol. 239:385-400(1994).
[9]	X-RAY CRYSTALLOGRAPHY (3.15 ANGSTROMS) OF 7S COMPLEX. STRAIN=Swiss Webster; TISSUE=Submandibular gland; DOI=10.1016/S0969-2126(97)00280-3; PubMed=9351801 [NCBI, ExPASy, EBI, Israel, Japan] Bax B., Blundell T.L., Murray-Rust J., McDonald N.Q.; "Structure of mouse 7S NGF: a complex of nerve growth factor with four binding proteins."; Structure 5:1275-1285(1997).

Comments

FUNCTION: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. It stimulates division and differentiation of sympathetic and embryonic sensory neurons.
SUBUNIT: Homodimer.
SUBCELLULAR LOCATION: Secreted.
SIMILARITY: Belongs to the NGF-beta family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

M35075; AAA39818.1; ALT_INIT; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
V00836; CAA24221.1; ALT_INIT; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
K01759; AAA39820.1; ALT_INIT; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
M14805; AAA39821.1; ALT_INIT; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
M17298; AAA37687.1; ALT_INIT; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
M17296; AAA37687.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
M17297; AAA37687.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
S62089; AAB26820.2; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_001106168.1; -.

UniGene

Mm.1259

3D structure databases

PDB

1BET; X-ray; 2.30 A; A=131-237.	[ExPASy / RCSB / EBI]
1BTG; X-ray; 2.50 A; A/B/C=130-239.	[ExPASy / RCSB / EBI]
1SGF; X-ray; 3.15 A; B/Y=122-239.	[ExPASy / RCSB / EBI]

Detailed list of linked structures.

PDBsum

1BET; -.
1BTG; -.
1SGF; -.

ModBase

P01139.

Organism-specific databases

MGI

MGI:97321; Ngf.

Gene expression databases

ArrayExpress

P01139; -.

CleanEx

MM_NGF; -.

GermOnline

ENSMUSG00000027859; Mus musculus.

Ontologies

GO:0005788;	Cellular component: endoplasmic reticulum lumen (inferred from experiment from Reactome).
GO:0005576;	Cellular component: extracellular region (inferred from experiment from Reactome).
GO:0005057;	Molecular function: receptor signaling protein activity (inferred from direct assay from MGI).
GO:0007422;	Biological process: peripheral nervous system development (inferred from mutant phenotype from MGI).
GO:0045773;	Biological process: positive regulation of axon extension (inferred from mutant phenotype from MGI).
GO:0045664;	Biological process: regulation of neuron differentiation (inferred from direct assay from MGI).
GO:0046928;	Biological process: regulation of neurotransmitter secretion (inferred from genetic interaction from MGI).
GO:0019233;	Biological process: sensory perception of pain (inferred from mutant phenotype from MGI).
QuickGo view.

Family and domain databases

InterPro

IPR002072; NGF.
Graphical view of domain structure.

PANTHER

PTHR11589; NGF; 1.

Pfam

PF00243; NGF; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF001789; NGF; 1.

PRINTS

PR00268; NGF.

ProDom

PD002052; NGF; 2.
[Domain structure / List of seq. sharing at least 1 domain]

SMART

SM00140; NGF; 1.
SMART graphical view of domain structure.

PROSITE

PS00248; NGF_1; 1.
PS50270; NGF_2; 1.
PROSITE graphical view of domain structure (profiles).

Other

Genome annotation databases

Ensembl

ENSMUSG00000027859; Mus musculus. [Contig view]

GeneID

18049; -.

KEGG

mmu:18049; -.

Phylogenomic databases

HOVERGEN

P01139; -.

Other

P01139; -.

Ngf; Mus musculus.

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

3D-structure; Cleavage on pair of basic residues; Direct protein sequencing; Glycoprotein; Growth factor; Phosphoprotein; Secreted; Signal.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`SIGNAL`	`1 18`	`18`	`Potential.`
`PROPEP`	`19 121`	`103`		`PRO_0000019601`
`CHAIN`	`122 241`	`120`	`Beta-nerve growth factor.`	`PRO_0000019602`
`MOD_RES`	`84 84`		`Phosphoserine (By similarity).`
`CARBOHYD`	`69 69`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`114 114`		`N-linked (GlcNAc...) (Potential).`
`DISULFID`	`136 201`
`DISULFID`	`179 229`
`DISULFID`	`189 231`
`CONFLICT`	`233 241`		`LSRKATRRG -> CSAGRLQEEADLPAAPFPTCPLHTLLGPSLPQPVNYFKL (in Ref. 5; AAB26820).`
`STRAND`	`133 136`	`4`
`STRAND`	`138 144`	`7`
`STRAND`	`148 151`	`4`
`STRAND`	`156 159`	`4`
`STRAND`	`161 165`	`5`
`STRAND`	`168 171`	`4`
`STRAND`	`174 180`	`7`
`TURN`	`194 196`	`3`
`STRAND`	`197 213`	`17`
`STRAND`	`218 235`	`18`

Sequence information

Length: 241 AA [This is the length of the unprocessed precursor]

Molecular weight: 27077 Da [This is the MW of the unprocessed precursor]

CRC64: 164465E1DC550081 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSMLFYTLIT AFLIGVQAEP YTDSNVPEGD SVPEAHWTKL QHSLDTALRR ARSAPTAPIA 

        70         80         90        100        110        120 
ARVTGQTRNI TVDPRLFKKR RLHSPRVLFS TQPPPTSSDT LDLDFQAHGT IPFNRTHRSK 

       130        140        150        160        170        180 
RSSTHPVFHM GEFSVCDSVS VWVGDKTTAT DIKGKEVTVL AEVNINNSVF RQYFFETKCR 

       190        200        210        220        230        240 
ASNPVESGCR GIDSKHWNSY CTTTHTFVKA LTTDEKQAAW RFIRIDTACV CVLSRKATRR 


G

P01139 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools