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UniProtKB/Swiss-Prot entry P01100


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name FOS_HUMAN
Primary accession number P01100
Secondary accession number P18849
Integrated into Swiss-Prot on July 21, 1986
Sequence was last modified on July 21, 1986 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 102)
Name and origin of the protein
Protein name Proto-oncogene protein c-fos
Synonyms Cellular oncogene fos
G0/G1 switch regulatory protein 7
Gene name
Name: FOS
Synonyms: G0S7
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=6574479 [NCBI, ExPASy, EBI, Israel, Japan]
van Straaten F., Mueller R., Curran T., Van Beveren C., Verma I.M.;
"Complete nucleotide sequence of a human c-onc gene: deduced amino acid sequence of the human c-fos protein.";
Proc. Natl. Acad. Sci. U.S.A. 80:3183-3187(1983).
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 133-200.
PubMed=2516827 [NCBI, ExPASy, EBI, Israel, Japan]
Hai T., Liu F., Coukos W.J., Green M.R.;
"Transcription factor ATF cDNA clones: an extensive family of leucine zipper proteins able to selectively form DNA-binding heterodimers.";
Genes Dev. 3:2083-2090(1989).
[3]
ERRATUM.
Hai T., Liu F., Coukos W.J., Green M.R.;
Genes Dev. 4:682-682(1990).
[4]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Rieder M.J., Livingston R.J., Daniels M.R., Montoya M.A., Chung M.-W., Miyamoto K.E., Nguyen C.P., Nguyen D.A., Poel C.L., Robertson P.D., Schackwitz W.S., Sherwood J.K., Witrak L.A., Nickerson D.A.;
"NIEHS-SNPs, environmental genome project, NIEHS ES15478, Department of Genome Sciences, Seattle, WA (URL: http://egp.gs.washington.edu).";
Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1038/nature01348; PubMed=12508121 [NCBI, ExPASy, EBI, Israel, Japan]
Heilig R., Eckenberg R., Petit J.-L., Fonknechten N., Da Silva C., Cattolico L., Levy M., Barbe V., De Berardinis V., Ureta-Vidal A., Pelletier E., Vico V., Anthouard V., Rowen L., Madan A., Qin S., Sun H., Du H., Pepin K., Artiguenave F., Robert C., Cruaud C., Bruels T., Jaillon O., Friedlander L., Samson G., Brottier P., Cure S., Segurens B., Aniere F., Samain S., Crespeau H., Abbasi N., Aiach N., Boscus D., Dickhoff R., Dors M., Dubois I., Friedman C., Gouyvenoux M., James R., Madan A., Mairey-Estrada B., Mangenot S., Martins N., Menard M., Oztas S., Ratcliffe A., Shaffer T., Trask B., Vacherie B., Bellemere C., Belser C., Besnard-Gonnet M., Bartol-Mavel D., Boutard M., Briez-Silla S., Combette S., Dufosse-Laurent V., Ferron C., Lechaplais C., Louesse C., Muselet D., Magdelenat G., Pateau E., Petit E., Sirvain-Trukniewicz P., Trybou A., Vega-Czarny N., Bataille E., Bluet E., Bordelais I., Dubois M., Dumont C., Guerin T., Haffray S., Hammadi R., Muanga J., Pellouin V., Robert D., Wunderle E., Gauguet G., Roy A., Sainte-Marthe L., Verdier J., Verdier-Discala C., Hillier L.W., Fulton L., McPherson J., Matsuda F., Wilson R., Scarpelli C., Gyapay G., Wincker P., Saurin W., Quetier F., Waterston R., Hood L., Weissenbach J.;
"The DNA sequence and analysis of human chromosome 14.";
Nature 421:601-607(2003).
[6]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Pancreas;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[7]
NUCLEOTIDE SEQUENCE [MRNA] OF 1-6.
PubMed=1658710 [NCBI, ExPASy, EBI, Israel, Japan]
Roux P., Verrier B., Klein B., Niccolino M., Marty L., Alexandre C., Piechaczyk M.;
"Retrovirus-mediated gene transfer of a human c-fos cDNA into mouse bone marrow stromal cells.";
Oncogene 6:2155-2160(1991).
[8]
DNA-BINDING.
PubMed=2511004 [NCBI, ExPASy, EBI, Israel, Japan]
Nakabeppu Y., Nathans D.;
"The basic region of Fos mediates specific DNA binding.";
EMBO J. 8:3833-3841(1989).
[9]
UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-113, AND MASS SPECTROMETRY.
TISSUE=Lung adenocarcinoma;
DOI=10.1021/pr060438j; PubMed=17203973 [NCBI, ExPASy, EBI, Israel, Japan]
Vasilescu J., Zweitzig D.R., Denis N.J., Smith J.C., Ethier M., Haines D.S., Figeys D.;
"The proteomic reactor facilitates the analysis of affinity-purified proteins by mass spectrometry: application for identifying ubiquitinated proteins in human cells.";
J. Proteome Res. 6:298-305(2007).
[10]
X-RAY CRYSTALLOGRAPHY (3.05 ANGSTROMS) OF 139-198 OF COMPLEX WITH JUN.
DOI=10.1038/373257a0; PubMed=7816143 [NCBI, ExPASy, EBI, Israel, Japan]
Glover J.N., Harrison S.C.;
"Crystal structure of the heterodimeric bZIP transcription factor c-Fos-c-Jun bound to DNA.";
Nature 373:257-261(1995).
Comments
  • FUNCTION: Nuclear phosphoprotein which forms a tight but non-covalently linked complex with the JUN/AP-1 transcription factor. In the heterodimer, c-fos and JUN/AP-1 basic regions each seems to interact with symmetrical DNA half sites. Has a critical function in regulating the development of cells destined to form and maintain the skeleton. It is thought to have an important role in signal transduction, cell proliferation and differentiation.
  • SUBUNIT: Heterodimer with JUN. Interacts with DSIPI; this interaction inhibits the binding of active AP1 to its target DNA. Interacts with MAFB (By similarity).
  • INTERACTION:
    O60869-1:EDF1; NbExp=1; IntAct=EBI-852851, EBI-781310;
  • SUBCELLULAR LOCATION: Nucleus.
  • INDUCTION: C-fos expression increases upon a variety of stimuli, including growth factors, cytokines, neurotransmitters, polypeptide hormones, stress and cell injury.
  • SIMILARITY: Belongs to the bZIP family. Fos subfamily.
  • SIMILARITY: Contains 1 bZIP domain.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
V01512; CAA24756.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
K00650; AAA52471.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY212879; AAO21129.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF111167; AAC98315.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC004490; AAH04490.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
S65138; AAB20306.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR A01342; TVHUF1.
E34223; E34223.
RefSeq NP_005243.1; -.
UniGene Hs.25647
3D structure databases
PDB
1A02; X-ray; 2.70 A; F=139-193.[ExPASy / RCSB / EBI]
1FOS; X-ray; 3.05 A; E=139-198, G=140-198.[ExPASy / RCSB / EBI]
1S9K; X-ray; 3.10 A; D=140-192.[ExPASy / RCSB / EBI]
Detailed list of linked structures.
PDBsum 1A02; -.
1FOS; -.
1S9K; -.
DisProt DP00078; -.
ModBase P01100.
Protein-protein interaction databases
DIP DIP:1047N; -.
IntAct P01100; -.
PTM databases
PhosphoSite P01100; -.
Polymorphism databases
NIEHS-SNPs FOS.
Organism-specific databases
H-InvDB HIX0011826; -.
HGNC HGNC:3796; FOS.
GenAtlas FOS.
HPA CAB000461; -.
MIM 164810; gene. [NCBI / EBI]
PharmGKB PA134953249; -.
GeneCards P01100.
Gene expression databases
ArrayExpress P01100; -.
CleanEx HS_FOS; -.
GermOnline ENSG00000170345; Homo sapiens.
Ontologies
GO
GO:0005634; Cellular component: nucleus (traceable author statement from ProtInc).
GO:0005515; Molecular function: protein binding (inferred from physical interaction from IntAct).
GO:0003704; Molecular function: specific RNA polymerase II transcription factor activity (traceable author statement from ProtInc).
GO:0006306; Biological process: DNA methylation (traceable author statement from ProtInc).
GO:0006954; Biological process: inflammatory response (traceable author statement from ProtInc).
GO:0006357; Biological process: regulation of transcription from RNA polymerase II promoter (traceable author statement from ProtInc).
QuickGo view.
Family and domain databases
InterPro IPR011700; bZIP_2.
IPR000837; Leuzip_Fos.
IPR004827; TF_bZIP.
Graphical view of domain structure.
Pfam PF07716; bZIP_2; 1.
Pfam graphical view of domain structure.
PRINTS PR00042; LEUZIPPRFOS.
SMART SM00338; BRLZ; 1.
SMART graphical view of domain structure.
PROSITE PS50217; BZIP; 1.
PS00036; BZIP_BASIC; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS P01100.
Genome annotation databases
Ensembl ENSG00000170345; Homo sapiens. [Contig view]
GeneID 2353; -.
KEGG hsa:2353; -.
Phylogenomic databases
HOGENOM P01100; -.
HOVERGEN P01100; -.
Other
LinkHub P01100; -.
SOURCE FOS; Homo sapiens.
ProtoNet P01100.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; DNA-binding; Nucleus; Phosphoprotein; Proto-oncogene; Ubl conjugation.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   380  380     Proto-oncogene protein c-fos. PRO_0000076465
DOMAIN   165   193  29     Leucine-zipper. 
DNA_BIND   139   160  22     Basic motif. 
CROSSLNK   113   113        Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin). 
CONFLICT   133   144        SPEEEEKRRIRR -> ISRRRREKENPK (in Ref. 2). 
HELIX   141   191  51      
Sequence information
Length: 380 AA [This is the length of the unprocessed precursor] Molecular weight: 40695 Da [This is the MW of the unprocessed precursor] CRC64: 9E3B2969347C90C8 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MMFSGFNADY EASSSRCSSA SPAGDSLSYY HSPADSFSSM GSPVNAQDFC TDLAVSSANF 

        70         80         90        100        110        120 
IPTVTAISTS PDLQWLVQPA LVSSVAPSQT RAPHPFGVPA PSAGAYSRAG VVKTMTGGRA 

       130        140        150        160        170        180 
QSIGRRGKVE QLSPEEEEKR RIRRERNKMA AAKCRNRRRE LTDTLQAETD QLEDEKSALQ 

       190        200        210        220        230        240 
TEIANLLKEK EKLEFILAAH RPACKIPDDL GFPEEMSVAS LDLTGGLPEV ATPESEEAFT 

       250        260        270        280        290        300 
LPLLNDPEPK PSVEPVKSIS SMELKTEPFD DFLFPASSRP SGSETARSVP DMDLSGSFYA 

       310        320        330        340        350        360 
ADWEPLHSGS LGMGPMATEL EPLCTPVVTC TPSCTAYTSS FVFTYPEADS FPSCAAAHRK 

       370        380 
GSSSNEPSSD SLSSPTLLAL 

P01100 in FASTA format

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