[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. DOI=10.1038/308613a0; PubMed=6546784 [NCBI, ExPASy, EBI, Israel, Japan] Chin D.J., Gil G., Russell D.W., Liscum L., Luskey K.L., Basu S.K., Okayama H., Berg P., Goldstein J.L., Brown M.S.; "Nucleotide sequence of 3-hydroxy-3-methyl-glutaryl coenzyme A reductase, a glycoprotein of endoplasmic reticulum."; Nature 308:613-617(1984).
[2]	GENE STRUCTURE. DOI=10.1016/0092-8674(84)90549-X; PubMed=6088070 [NCBI, ExPASy, EBI, Israel, Japan] Reynolds G.A., Basu S.K., Osborne T.F., Chin D.J., Gil G., Brown M.S., Goldstein J.L., Luskey K.L.; "HMG CoA reductase: a negatively regulated gene with unusual promoter and 5' untranslated regions."; Cell 38:275-285(1984).
[3]	DOMAINS. PubMed=3965461 [NCBI, ExPASy, EBI, Israel, Japan] Liscum L., Finer-Moore J., Stroud R.M., Luskey K.L., Brown M.S., Goldstein J.L.; "Domain structure of 3-hydroxy-3-methylglutaryl coenzyme A reductase, a glycoprotein of the endoplasmic reticulum."; J. Biol. Chem. 260:522-530(1985).

Comments

FUNCTION: This transmembrane glycoprotein is involved in the control of cholesterol biosynthesis. It is the rate-limiting enzyme of sterol biosynthesis.
CATALYTIC ACTIVITY: (R)-mevalonate + CoA + 2 NADP⁺ = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH.
PATHWAY: Metabolic intermediate biosynthesis; mevalonic acid biosynthesis; (R)-mevalonic acid from acetyl-CoA: step 3/3 .
SUBUNIT: Homodimer (By similarity).
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein.
SIMILARITY: Belongs to the HMG-CoA reductase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

L00183; AAA36989.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
L00166; AAA36989.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
L00169; AAA36989.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
L00170; AAA36989.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
L00171; AAA36989.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
L00173; AAA36989.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
L00176; AAA36989.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
L00177; AAA36989.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
L00178; AAA36989.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
L00179; AAA36989.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
L00180; AAA36989.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
L00181; AAA36989.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
L00182; AAA36989.1; JOINED; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
X00494; CAA25189.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

A93328; RDHYE.

3D structure databases

HSSP

P04035; 1HWJ. [HSSP ENTRY / PDB]

SMR

P00347; 461-869.

ModBase

P00347.

Ontologies

GO:0005789;	Cellular component: endoplasmic reticulum membrane (inferred from electronic annotation from InterPro).
GO:0016021;	Cellular component: integral to membrane (inferred from electronic annotation from InterPro).
GO:0004420;	Molecular function: hydroxymethylglutaryl-CoA reductase (NADPH) activity (inferred from electronic annotation from InterPro).
GO:0050661;	Molecular function: NADP binding (inferred from electronic annotation from InterPro).
GO:0006695;	Biological process: cholesterol biosynthetic process (inferred from electronic annotation from UniProtKB-KW).
GO:0015936;	Biological process: coenzyme A metabolic process (inferred from electronic annotation from InterPro).
GO:0008299;	Biological process: isoprenoid biosynthetic process (inferred from electronic annotation from InterPro).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR002202; HMG_CoA_Rdtase_cat.
IPR004554; HMG_CoA_Rdtase_I_cat.
IPR004816; HMG_CoA_Rdtase_I_metazoan.
IPR000731; SSD_5TM.
Graphical view of domain structure.

Gene3D

G3DSA:3.90.770.10; HMG-CoA_red; 1.

PANTHER

PTHR10572; HMG-CoA_red; 1.

Pfam

PF00368; HMG-CoA_red; 1.
Pfam graphical view of domain structure.

PRINTS

PR00071; HMGCOARDTASE.

TIGRFAMs

TIGR00920; 2A060605; 1.
TIGR00533; HMG_CoA_R_NADP; 1.

PROSITE

PS00066; HMG_COA_REDUCTASE_1; 1.
PS00318; HMG_COA_REDUCTASE_2; 1.
PS01192; HMG_COA_REDUCTASE_3; 1.
PS50065; HMG_COA_REDUCTASE_4; 1.
PS50156; SSD; 1.
PROSITE graphical view of domain structure (profiles).

ProtoNet

P00347.

Phylogenomic databases

HOVERGEN

P00347; -.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cholesterol biosynthesis; Endoplasmic reticulum; Glycoprotein; Lipid synthesis; Membrane; NADP; Oxidoreductase; Steroid biosynthesis; Sterol biosynthesis; Transmembrane.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 887`	`887`	`3-hydroxy-3-methylglutaryl-coenzyme A reductase.`	`PRO_0000114418`
`TRANSMEM`	`10 39`	`30`
`TRANSMEM`	`57 78`	`22`
`TRANSMEM`	`90 114`	`25`
`TRANSMEM`	`124 149`	`26`
`TRANSMEM`	`160 187`	`28`
`TRANSMEM`	`192 220`	`29`
`TRANSMEM`	`315 339`	`25`
`REGION`	`340 449`	`110`	`Linker.`
`REGION`	`450 887`	`438`	`Catalytic.`
`ACT_SITE`	`558 558`		`Charge relay system (By similarity).`
`ACT_SITE`	`690 690`		`Charge relay system (By similarity).`
`ACT_SITE`	`766 766`		`Charge relay system (By similarity).`
`ACT_SITE`	`865 865`		`Proton donor (By similarity).`
`CARBOHYD`	`281 281`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`517 517`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`869 869`		`N-linked (GlcNAc...) (Potential).`
`VARIANT`	`733 733`	`1`	`N -> C.`

Sequence information

Length: 887 AA [This is the length of the unprocessed precursor]

Molecular weight: 97081 Da [This is the MW of the unprocessed precursor]

CRC64: 4331E53ADA250E6A [This is a checksum on the sequence]

        10         20         30         40         50         60 
MLSRLFRMHG LFVASHPWEV IVGTVTLTIC MMSMNMFTGN NKICGWNYEC PKFEEDVLSS 

        70         80         90        100        110        120 
DIIILTITRC IAILYIYFQF QNLRQLGSKY ILGIAGLFTI FSSFVFSTVV IHFLDKELTG 

       130        140        150        160        170        180 
LNEALPFFLL LIDLSRASAL AKFALSSNSQ DEVRENIARG MAILGPTFTL DALVECLVIG 

       190        200        210        220        230        240 
VGTMSGVRQL EIMCCFGCMS VLANYFVFMT FFPACVSLVL ELSRESREGR PIWQLSHFAR 

       250        260        270        280        290        300 
VLEEEENKPN PVTQRVKMIM SLGLVLVHAH SRWIADPSPQ NSTTEHSKVS LGLDEDVSKR 

       310        320        330        340        350        360 
IEPSVSLWQF YLSKMISMDI EQVVTLSLAF LLAVKYIFFE QAETESTLSL KNPITSPVVT 

       370        380        390        400        410        420 
PKKAPDNCCR REPLLVRRSE KLSSVEEEPG VSQDRKVEVI KPLVVETESA SRATFVLGAS 

       430        440        450        460        470        480 
GTSPPVAART QELEIELPSE PRPNEECLQI LESAEKGAKF LSDAEIIQLV NAKHIPAYKL 

       490        500        510        520        530        540 
ETLMETHERG VSIRRQLLST KLPEPSSLQY LPYRDYNYSL VMGACCENVI GYMPIPVGVA 

       550        560        570        580        590        600 
GPLCLDGKEY QVPMATTEGC LVASTNRGCR AIGLGGGASS RVLADGMTRG PVVRLPRACD 

       610        620        630        640        650        660 
SAEVKAWLET PEGFAVIKDA FDSTSRFARL QKLHVTMAGR NLYIRFQSKT GDAMGMNMIS 

       670        680        690        700        710        720 
KGTEKALLKL QEFFPEMQIL AVSGNYCTDK KPAAINWIEG RGKTVVCEAV IPAKVVREVL 

       730        740        750        760        770        780 
KTTTEAMIDV NINKNLVGSA MAGSIGGYNA HAANIVTAIY IACGQDAAQN VGSSNCITLM 

       790        800        810        820        830        840 
EASGPTNEDL YISCTMPSIE IGTVGGGTNL LPQQACLQML GVQGACKDNP GENARQLARI 

       850        860        870        880 
VCGTVMAGEL SLMAALAAGH LVRSHMVHNR SKINLQDLQG TCTKKSA

P00347 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools