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UniProtKB/Swiss-Prot entry O95881


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name TXD12_HUMAN
Primary accession number O95881
Secondary accession numbers Q5T1T4 Q96H50
Integrated into Swiss-Prot on April 11, 2003
Sequence was last modified on May 1, 1999 (Sequence version 1)
Annotations were last modified on    November 25, 2008 (Entry version 71)
Name and origin of the protein
Protein name Thioredoxin domain-containing protein 12 [Precursor]
Synonyms EC 1.8.4.2
Thioredoxin-like protein p19
Endoplasmic reticulum protein ERp19
ERp18
hTLP19
Gene name
Name: TXNDC12
Synonyms: TLP19
ORFNames: UNQ713/PRO1376
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
DOI=10.1016/S0378-1119(03)00732-7; PubMed=14557066 [NCBI, ExPASy, EBI, Israel, Japan]
Liu F., Rong Y.P., Zeng L.C., Zhang X., Han Z.G.;
"Isolation and characterization of a novel human thioredoxin-like gene hTLP19 encoding a secretory protein.";
Gene 315:71-78(2003).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Brain;
Mei G., Yu W., Gibbs R.A.;
Submitted (FEB-1999) to the EMBL/GenBank/DDBJ databases.
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
DOI=10.1101/gr.1293003; PubMed=12975309 [NCBI, ExPASy, EBI, Israel, Japan]
Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.;
"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.";
Genome Res. 13:2265-2270(2003).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1038/nature04727; PubMed=16710414 [NCBI, ExPASy, EBI, Israel, Japan]
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K., Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.;
"The DNA sequence and biological annotation of human chromosome 1.";
Nature 441:315-321(2006).
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Colon, Kidney, and Ovary;
DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan]
The MGC Project Team;
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).";
Genome Res. 14:2121-2127(2004).
[6]
PROTEIN SEQUENCE OF 27-41.
DOI=10.1110/ps.04682504; PubMed=15340161 [NCBI, ExPASy, EBI, Israel, Japan]
Zhang Z., Henzel W.J.;
"Signal peptide prediction based on analysis of experimentally verified cleavage sites.";
Protein Sci. 13:2819-2824(2004).
[7]
FUNCTION, SUBCELLULAR LOCATION, DISULFIDE BOND, AND MUTAGENESIS OF CYS-66 AND CYS-69.
DOI=10.1074/jbc.M304598200; PubMed=12761212 [NCBI, ExPASy, EBI, Israel, Japan]
Alanen H.I., Williamson R.A., Howard M.J., Lappi A.-K., Jaentti H.P., Rautio S.M., Kellokumpu S., Ruddock L.W.;
"Functional characterization of ERp18, a new endoplasmic reticulum-located thioredoxin superfamily member.";
J. Biol. Chem. 278:28912-28920(2003).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AF543416; AAN34781.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF131758; AAD20035.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY358982; AAQ89341.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AL445685; CAI17031.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC001493; AAH01493.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC008953; AAH08953.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
BC008913; AAH08913.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_056997.1; -.
UniGene Hs.476033
3D structure databases
PDB
1SEN; X-ray; 1.20 A; A=23-172.[ExPASy / RCSB / EBI]
PDBsum 1SEN; -.
SMR O95881; 30-163.
ModBase O95881.
PTM databases
PhosphoSite O95881; -.
Organism-specific databases
H-InvDB HIX0000579; -.
HIX0029114; -.
HGNC HGNC:24626; TXNDC12.
GenAtlas TXNDC12.
MIM 609448; gene. [NCBI / EBI]
PharmGKB PA142670665; -.
GeneCards O95881.
Gene expression databases
ArrayExpress O95881; -.
CleanEx HS_TXNDC12; -.
GermOnline ENSG00000117862; Homo sapiens.
Ontologies
GO
GO:0005783; Cellular component: endoplasmic reticulum (inferred from electronic annotation from UniProtKB-KW).
GO:0005788; Cellular component: endoplasmic reticulum lumen (inferred from electronic annotation from UniProtKB-SubCell).
GO:0019153; Molecular function: protein-disulfide reductase (glutathione) activity (inferred from electronic annotation from EC).
GO:0045454; Biological process: cell redox homeostasis (inferred from electronic annotation from InterPro).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR006662; Thioredoxin-like.
IPR012335; Thioredoxin_fold.
Graphical view of domain structure.
Gene3D G3DSA:3.40.30.10; Thioredoxin_fold; 1.
PROSITE PS00194; THIOREDOXIN_1; 1.
PS51352; THIOREDOXIN_2; 1.
PROSITE graphical view of domain structure (profiles).
ProtoNet O95881.
Proteomic databases
PeptideAtlas O95881; -.
Genome annotation databases
Ensembl ENSG00000117862; Homo sapiens. [Contig view]
GeneID 51060; -.
KEGG hsa:51060; -.
Phylogenomic databases
HOGENOM O95881; -.
HOVERGEN O95881; -.
Other
DrugBank DB00143; Glutathione.
NextBio 53641; -.
SOURCE TXNDC12; Homo sapiens.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Direct protein sequencing; Endoplasmic reticulum; Oxidoreductase; Redox-active center; Signal.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
SIGNAL   1    26  26      
CHAIN   27   172  146     Thioredoxin domain-containing protein 12. PRO_0000034189
MOTIF   169   172  4     Prevents secretion from ER (Potential). 
DISULFID   66    69        Redox-active. 
MUTAGEN   66    66        C->S: Loss of oxidase activity. 
MUTAGEN   69    69        C->S: Loss of oxidase activity. 
CONFLICT   102   102        D -> H (in Ref. 5; AAH08913). 
HELIX   43    53  11      
STRAND   57    62  6      
HELIX   67    77  11      
HELIX   80    86  7      
STRAND   89    95  7      
HELIX   96    98  3      
HELIX   103   105  3      
STRAND   112   118  7      
HELIX   144   158  15      
HELIX   159   161  3      
Sequence information
Length: 172 AA [This is the length of the unprocessed precursor] Molecular weight: 19206 Da [This is the MW of the unprocessed precursor] CRC64: 3092E9515A7C4094 [This is a checksum on the sequence]
        10         20         30         40         50         60 
METRPRLGAT CLLGFSFLLL VISSDGHNGL GKGFGDHIHW RTLEDGKKEA AASGLPLMVI 

        70         80         90        100        110        120 
IHKSWCGACK ALKPKFAEST EISELSHNFV MVNLEDEEEP KDEDFSPDGG YIPRILFLDP 

       130        140        150        160        170 
SGKVHPEIIN ENGNPSYKYF YVSAEQVVQG MKEAQERLTG DAFRKKHLED EL 

O95881 in FASTA format

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BLAST logo BLAST submission on ExPASy/SIB
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Tools Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
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