[1]	NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). TISSUE=Brain; PubMed=10233149 [NCBI, ExPASy, EBI, Israel, Japan] Yuan W., Stromhaug P.E., Dunn W.A. Jr.; "Glucose-induced autophagy of peroxisomes in Pichia pastoris requires a unique E1-like protein."; Mol. Biol. Cell 10:1353-1366(1999).
[2]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). TISSUE=Placenta; DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan] The MGC Project Team; "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."; Genome Res. 14:2121-2127(2004).
[3]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 179-703 (ISOFORM 1). TISSUE=Testis; DOI=10.1186/1471-2164-8-399; PubMed=17974005 [NCBI, ExPASy, EBI, Israel, Japan] Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Blocker H., Heubner D., Hoerlein A., Michel G., Wedler H., Kohrer K., Ottenwalder B., Poustka A., Wiemann S., Schupp I.; "The full-ORF clone resource of the German cDNA consortium."; BMC Genomics 8:399-399(2007).
[4]	TISSUE SPECIFICITY. DOI=10.1006/bbrc.2002.6645; PubMed=11890701 [NCBI, ExPASy, EBI, Israel, Japan] Tanida I., Tanida-Miyake E., Nishitani T., Komatsu M., Yamazaki H., Ueno T., Kominami E.; "Murine Apg12p has a substrate preference for murine Apg7p over three Apg8p homologs."; Biochem. Biophys. Res. Commun. 292:256-262(2002).
[5]	INTERACTION WITH ATG3 AND ATG12. TISSUE=Brain; DOI=10.1074/jbc.M200385200; PubMed=11825910 [NCBI, ExPASy, EBI, Israel, Japan] Tanida I., Tanida-Miyake E., Komatsu M., Ueno T., Kominami E.; "Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p."; J. Biol. Chem. 277:13739-13744(2002).
[6]	IDENTIFICATION [LARGE SCALE ANALYSIS], AND MASS SPECTROMETRY. Colinge J., Superti-Furga G., Bennett K.L.; Submitted (OCT-2008) to UniProtKB.

Comments

FUNCTION: Functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12. Forms intermediate conjugates with GABARAPL1 (GABARAPL2, GABARAP or MAP1ALC3). Formation of the final GABARAPL1-PE conjugate is essential for autophagy (By similarity).
SUBUNIT: Homodimer (By similarity). Interacts with ATG3 and ATG12. The complex, composed of ATG3 and ATG7, plays a role in the conjugation of ATG12 to ATG5.
SUBCELLULAR LOCATION: Cytoplasm (Probable).
ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative splicing.

Name 1

Isoform ID O95352-1

This is the isoform sequence displayed in this entry.

Name 2

Isoform ID O95352-2

Features which should be applied to build the isoform sequence: VSP_013205.
TISSUE SPECIFICITY: Widely expressed, especially in kidney, liver, lymph nodes and bone marrow.
DOMAIN: The C-terminal part of the protein is essential for the dimerization and interaction with ATG3 and ATG12 (By similarity).
SIMILARITY: Belongs to the ATG7 family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AF094516; AAC69630.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BC000091; AAH00091.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AL122075; CAB59250.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

IPI

IPI00007404; -.
IPI00479911; -.

PIR

T34556; T34556.

RefSeq

NP_001129503.2; -.
NP_006386.1; -.

UniGene

Hs.38032

3D structure databases

HSSP

P12282; 1JW9. [HSSP ENTRY / PDB]

ModBase

O95352.

Protein-protein interaction databases

IntAct

O95352; 3.

PTM databases

PhosphoSite

O95352; -.

Organism-specific databases

GC03P011290; -.

HIX0003055; -.

HGNC:16935; ATG7.

CAB018771; -.
HPA007639; -.

MIM

608760; gene. [NCBI / EBI]

PharmGKB

PA134983397; -.

Gene expression databases

O95352; -.

O95352; -.

HS_ATG7; -.

ENSG00000197548; Homo sapiens.

Ontologies

GO:0005737;	Cellular component: cytoplasm (inferred from direct assay from HPA).
GO:0042803;	Molecular function: protein homodimerization activity (inferred from direct assay from UniProtKB).
GO:0004839;	Molecular function: ubiquitin activating enzyme activity (traceable author statement from ProtInc).
GO:0006914;	Biological process: autophagy (inferred from electronic annotation from UniProtKB-KW).
GO:0006944;	Biological process: membrane fusion (traceable author statement from ProtInc).
GO:0019941;	Biological process: modification-dependent protein catabolic process (inferred from electronic annotation from UniProtKB-KW).
GO:0031401;	Biological process: positive regulation of protein modification process (inferred from direct assay from MGI).
GO:0006497;	Biological process: protein amino acid lipidation (inferred from direct assay from MGI).
GO:0015031;	Biological process: protein transport (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR006285; E1-like_Apg7.
IPR016040; NAD(P)-bd_dom.
IPR000594; ThiF_NAD_FAD_bd.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.50.720; NAD(P)-bd; 1.

Pfam

PF00899; ThiF; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR01381; E1_like_apg7; 1.

Proteomic databases

PRIDE

O95352; -.

Genome annotation databases

Ensembl

ENSG00000197548; Homo sapiens. [Contig view]

GeneID

10533; -.

Phylogenomic databases

HOGENOM

O95352; -.

HOVERGEN

O95352; -.

OMA

O95352; DQYEREG.

Other

39961; -.

ATG7; Homo sapiens.

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Alternative splicing; Autophagy; Cytoplasm; Polymorphism; Protein transport; Transport; Ubl conjugation pathway.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 703`	`703`	`Autophagy-related protein 7.`	`PRO_0000212806`
`ACT_SITE`	`572 572`		`Glycyl thioester intermediate (Potential).`
`VAR_SEQ`	`626 652`		`Missing (in isoform 2).`	`VSP_013205`
`VARIANT`	`471 471`	`1`	`V -> A (in dbSNP:rs36117895 [NCBI]).`	`VAR_053014`

Sequence information

Length: 703 AA [This is the length of the unprocessed precursor]

Molecular weight: 77960 Da [This is the MW of the unprocessed precursor]

CRC64: ABBD1A29A6C58356 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAAATGDPGL SKLQFAPFSS ALDVGFWHEL TQKKLNEYRL DEAPKDIKGY YYNGDSAGLP 

        70         80         90        100        110        120 
ARLTLEFSAF DMSAPTPARC CPAIGTLYNT NTLESFKTAD KKLLLEQAAN EIWESIKSGT 

       130        140        150        160        170        180 
ALENPVLLNK FLLLTFADLK KYHFYYWFCY PALCLPESLP LIQGPVGLDQ RFSLKQIEAL 

       190        200        210        220        230        240 
ECAYDNLCQT EGVTALPYFL IKYDENMVLV SLLKHYSDFF QGQRTKITIG VYDPCNLAQY 

       250        260        270        280        290        300 
PGWPLRNFLV LAAHRWSSSF QSVEVVCFRD RTMQGARDVA HSIIFEVKLP EMAFSPDCPK 

       310        320        330        340        350        360 
AVGWEKNQKG GMGPRMVNLS ECMDPKRLAE SSVDLNLKLM CWRLVPTLDL DKVVSVKCLL 

       370        380        390        400        410        420 
LGAGTLGCNV ARTLMGWGVR HITFVDNAKI SYSNPVRQPL YEFEDCLGGG KPKALAAADR 

       430        440        450        460        470        480 
LQKIFPGVNA RGFNMSIPMP GHPVNFSSVT LEQARRDVEQ LEQLIESHDV VFLLMDTRES 

       490        500        510        520        530        540 
RWLPAVIAAS KRKLVINAAL GFDTFVVMRH GLKKPKQQGA GDLCPNHPVA SADLLGSSLF 

       550        560        570        580        590        600 
ANIPGYKLGC YFCNDVVAPG DSTRDRTLDQ QCTVSRPGLA VIAGALAVEL MVSVLQHPEG 

       610        620        630        640        650        660 
GYAIASSSDD RMNEPPTSLG LVPHQIRGFL SRFDNVLPVS LAFDKCTACS SKVLDQYERE 

       670        680        690        700 
GFNFLAKVFN SSHSFLEDLT GLTLLHQETQ AAEIWDMSDD ETI

O95352 in FASTA format

View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools