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UniProtKB/Swiss-Prot entry O95166


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name GBRAP_HUMAN
Primary accession number O95166
Secondary accession numbers None
Integrated into Swiss-Prot on March 1, 2004
Sequence was last modified on May 1, 1999 (Sequence version 1)
Annotations were last modified on    June 16, 2009 (Entry version 69)
Name and origin of the protein
Protein name Gamma-aminobutyric acid receptor-associated protein
Synonyms GABA(A) receptor-associated protein
MM46
Gene name
Name: GABARAP
Synonyms: FLC3B
ORFNames: HT004
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND INTERACTION WITH GABRG2 AND BETA-TUBULIN.
TISSUE=Brain;
DOI=10.1038/16264; PubMed=9892355 [NCBI, ExPASy, EBI, Israel, Japan]
Wang H., Bedford F.K., Brandon N.J., Moss S.J., Olsen R.W.;
"GABA(A)-receptor-associated protein links GABA(A) receptors and the cytoskeleton.";
Nature 397:69-72(1999).
[2]
NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, AND INTERACTION WITH ULK1.
TISSUE=Frontal cortex;
DOI=10.1016/S0169-328X(00)00218-7; PubMed=11146101 [NCBI, ExPASy, EBI, Israel, Japan]
Okazaki N., Yan J., Yuasa S., Ueno T., Kominami E., Masuho Y., Koga H., Muramatsu M.-A.;
"Interaction of the Unc-51-like kinase and microtubule-associated protein light chain 3 related proteins in the brain: possible role of vesicular transport in axonal elongation.";
Brain Res. Mol. Brain Res. 85:1-12(2000).
[3]
NUCLEOTIDE SEQUENCE [MRNA].
Iijima M., Mitsui Y.;
Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases.
[4]
NUCLEOTIDE SEQUENCE [MRNA].
Ye M., Fu G., Wu J., Zhou J., Zhang Q., Shen Y., Kan L., He K., Gu B., Chen S., Mao M., Chen Z.;
Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases.
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Hypothalamus;
DOI=10.1073/pnas.160270997; PubMed=10931946 [NCBI, ExPASy, EBI, Israel, Japan]
Hu R.-M., Han Z.-G., Song H.-D., Peng Y.-D., Huang Q.-H., Ren S.-X., Gu Y.-J., Huang C.-H., Li Y.-B., Jiang C.-L., Fu G., Zhang Q.-H., Gu B.-W., Dai M., Mao Y.-F., Gao G.-F., Rong R., Ye M., Zhou J., Xu S.-H., Gu J., Shi J.-X., Jin W.-R., Zhang C.-K., Wu T.-M., Huang G.-Y., Chen Z., Chen M.-D., Chen J.-L.;
"Gene expression profiling in the human hypothalamus-pituitary-adrenal axis and full-length cDNA cloning.";
Proc. Natl. Acad. Sci. U.S.A. 97:9543-9548(2000).
[6]
IDENTIFICATION [LARGE SCALE ANALYSIS], AND MASS SPECTROMETRY.
Colinge J., Superti-Furga G., Bennett K.L.;
Submitted (OCT-2008) to UniProtKB.
[7]
X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS), AND INTERACTION WITH GABRG2; TUBULIN ALPHA AND TUBULIN BETA.
DOI=10.1074/jbc.M109753200; PubMed=11729197 [NCBI, ExPASy, EBI, Israel, Japan]
Knight D., Harris R., McAlister M.S.B., Phelan J.P., Geddes S., Moss S.J., Driscoll P.C., Keep N.H.;
"The X-ray crystal structure and putative ligand-derived peptide binding properties of gamma-aminobutyric acid receptor type A receptor-associated protein.";
J. Biol. Chem. 277:5556-5561(2002).
[8]
STRUCTURE BY NMR.
DOI=10.1074/jbc.C200050200; PubMed=11875056 [NCBI, ExPASy, EBI, Israel, Japan]
Stangler T., Mayr L.M., Willbold D.;
"Solution structure of human GABA(A) receptor-associated protein GABARAP: implications for biolgoical funcrion and its regulation.";
J. Biol. Chem. 277:13363-13366(2002).
[9]
STRUCTURE BY NMR.
DOI=10.1023/A:1013884402033; PubMed=11885988 [NCBI, ExPASy, EBI, Israel, Japan]
Kouno T., Miura K., Kanematsu T., Shirakawa M., Hirata M., Kawano K.;
"1H, 13C and '5N resonance assignments of GABARAP, GABAA receptor associated protein.";
J. Biomol. NMR 22:97-98(2002).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AF161586; AAD47641.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AB030711; BAB21549.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF044671; AAD02337.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF067171; AAD32455.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF183425; AAG09694.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
IPI IPI00027253; -.
RefSeq NP_009209.1; -.
UniGene Hs.647421
3D structure databases
PDB
1GNU; X-ray; 1.75 A; A=1-117.[ExPASy / RCSB / EBI]
1KLV; NMR; -; A=1-117.[ExPASy / RCSB / EBI]
1KM7; NMR; -; A=1-117.[ExPASy / RCSB / EBI]
1KOT; NMR; -; A=1-117.[ExPASy / RCSB / EBI]
3D32; X-ray; 1.30 A; A/B=1-117.[ExPASy / RCSB / EBI]
3DOW; X-ray; 2.30 A; A=1-117.[ExPASy / RCSB / EBI]
Detailed list of linked structures.
PDBsum 1GNU; -.
1KLV; -.
1KM7; -.
1KOT; -.
3D32; -.
3DOW; -.
ModBase O95166.
Protein-protein interaction databases
IntAct O95166; 13.
Protein family/group databases
TCDB 1.A.9.5.2; neurotransmitter receptor, cys loop, ligand-gated ion channel (LIC) family.
PTM databases
PhosphoSite O95166; -.
Organism-specific databases
GeneCards GC17M007084; -.
H-InvDB HIX0013108; -.
HGNC HGNC:4067; GABARAP.
GenAtlas GABARAP.
MIM 605125; gene. [NCBI / EBI]
PharmGKB PA28480; -.
Gene expression databases
ArrayExpress O95166; -.
Bgee O95166; -.
CleanEx HS_GABARAP; -.
GermOnline ENSG00000170296; Homo sapiens.
Ontologies
GO
GO:0000421; Cellular component: autophagic vacuole membrane (inferred from direct assay from UniProtKB).
GO:0000139; Cellular component: Golgi membrane (inferred from electronic annotation from UniProtKB-SubCell).
GO:0005874; Cellular component: microtubule (inferred from electronic annotation from UniProtKB-KW).
GO:0005886; Cellular component: plasma membrane (traceable author statement from ProtInc).
GO:0048487; Molecular function: beta-tubulin binding (inferred from direct assay from UniProtKB).
GO:0050811; Molecular function: GABA receptor binding (inferred from physical interaction from UniProtKB).
GO:0006605; Biological process: protein targeting (traceable author statement from ProtInc).
GO:0007268; Biological process: synaptic transmission (traceable author statement from ProtInc).
QuickGo view.
Family and domain databases
InterPro IPR004241; MAP1_LC3.
Graphical view of domain structure.
PANTHER PTHR10969; MAP1_LC3; 1.
Pfam PF02991; MAP1_LC3; 1.
Pfam graphical view of domain structure.
Genome annotation databases
Ensembl ENSG00000170296; Homo sapiens. [Contig view]
GeneID 11337; -.
KEGG hsa:11337; -.
Phylogenomic databases
HOGENOM O95166; -.
HOVERGEN O95166; -.
OMA O95166; TTMGQLY.
Other
NextBio 43075; -.
PMAP-CutDB O95166; -.
SOURCE GABARAP; Homo sapiens.
ProtoNet O95166.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Cytoplasm; Cytoskeleton; Golgi apparatus; Membrane; Microtubule; Protein transport; Transport.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   117  117     Gamma-aminobutyric acid receptor-associated protein. PRO_0000212363
REGION   1    22  22     Interaction with beta-tubulin. 
REGION   36   117  82     Interaction with GPHN (By similarity). 
REGION   36    68  33     Interaction with GABRG2 (Potential). 
HELIX   4     8  5      
HELIX   11    24  14      
STRAND   28    35  8      
STRAND   48    52  5      
HELIX   57    67  11      
STRAND   77    80  4      
HELIX   91    98  8      
STRAND   105   111  7      
Sequence information
Length: 117 AA [This is the length of the unprocessed precursor] Molecular weight: 13918 Da [This is the MW of the unprocessed precursor] CRC64: BC0B84B8A51C1E32 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MKFVYKEEHP FEKRRSEGEK IRKKYPDRVP VIVEKAPKAR IGDLDKKKYL VPSDLTVGQF 

        70         80         90        100        110 
YFLIRKRIHL RAEDALFFFV NNVIPPTSAT MGQLYQEHHE EDFFLYIAYS DESVYGL 

O95166 in FASTA format

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