ID   ATG12_HUMAN             Reviewed;         140 AA.
AC   O94817; Q6PJV2;
DT   16-NOV-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   25-NOV-2008, entry version 62.
DE   RecName: Full=Autophagy-related protein 12;
DE   AltName: Full=APG12-like;
GN   Name=ATG12; Synonyms=APG12, APG12L;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), CONJUGATION TO ATG5, AND
RP   TISSUE SPECIFICITY.
RX   MEDLINE=99069367; PubMed=9852036; DOI=10.1074/jbc.273.51.33889;
RA   Mizushima N., Sugita H., Yoshimori T., Ohsumi Y.;
RT   "A new protein conjugation system in human. The counterpart of the
RT   yeast Apg12p conjugation system essential for autophagy.";
RL   J. Biol. Chem. 273:33889-33892(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC   TISSUE=Brain, and Ovary;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Required for autophagy.
CC   -!- SUBUNIT: Conjugated to ATG5.
CC   -!- INTERACTION:
CC       Q9H0Y0:ATG10; NbExp=1; IntAct=EBI-746742, EBI-1048913;
CC       Q676U5:ATG16L1; NbExp=1; IntAct=EBI-746742, EBI-535909;
CC       Q9H1Y0:ATG5; NbExp=1; IntAct=EBI-746742, EBI-1047414;
CC       Q9H2U1:DHX36; NbExp=1; IntAct=EBI-746742, EBI-1047643;
CC       Q01804:OTUD4; NbExp=1; IntAct=EBI-746742, EBI-1054396;
CC       Q15459:SF3A1; NbExp=1; IntAct=EBI-746742, EBI-1054743;
CC       O75533:SF3B1; NbExp=1; IntAct=EBI-746742, EBI-876542;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=O94817-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=O94817-4; Sequence=VSP_018104, VSP_018105;
CC         Note=No experimental confirmation available;
CC   -!- TISSUE SPECIFICITY: Ubiquitous.
CC   -!- SIMILARITY: Belongs to the ATG12 family.
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DR   EMBL; AB017507; BAA36493.1; -; mRNA.
DR   EMBL; BC011033; AAH11033.1; -; mRNA.
DR   EMBL; BC012266; AAH12266.2; ALT_INIT; mRNA.
DR   RefSeq; NP_004698.2; -.
DR   UniGene; Hs.264482; -.
DR   IntAct; O94817; -.
DR   Ensembl; ENSG00000145782; Homo sapiens.
DR   GeneID; 9140; -.
DR   KEGG; hsa:9140; -.
DR   H-InvDB; HIX0005105; -.
DR   HGNC; HGNC:588; ATG12.
DR   HPA; CAB013272; -.
DR   MIM; 609608; gene.
DR   PharmGKB; PA24879; -.
DR   HOGENOM; O94817; -.
DR   HOVERGEN; O94817; -.
DR   LinkHub; O94817; -.
DR   NextBio; 34279; -.
DR   ArrayExpress; O94817; -.
DR   CleanEx; HS_ATG12; -.
DR   GermOnline; ENSG00000145782; Homo sapiens.
DR   GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR   GO; GO:0043231; C:intracellular membrane-bounded organelle; ISS:UniProtKB.
DR   GO; GO:0005515; F:protein binding; IPI:UniProtKB.
DR   GO; GO:0000045; P:autophagosome formation; ISS:UniProtKB.
DR   GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR007242; Autophagy_prot_12.
DR   PANTHER; PTHR13385; APG12; 1.
DR   Pfam; PF04110; APG12; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; Autophagy; Cytoplasm; Ubl conjugation pathway.
FT   CHAIN         1    140       Autophagy-related protein 12.
FT                                /FTId=PRO_0000212471.
FT   CROSSLNK    140    140       Glycyl lysine isopeptide (Gly-Lys)
FT                                (interchain with K-130 in ATG5).
FT   VAR_SEQ      56     74       DILLKAVGDTPIMKTKKWA -> YLCESVLCSFPRPRSWNS
FT                                L (in isoform 2).
FT                                /FTId=VSP_018104.
FT   VAR_SEQ      75    140       Missing (in isoform 2).
FT                                /FTId=VSP_018105.
SQ   SEQUENCE   140 AA;  15113 MW;  109258521C2D194F CRC64;
     MAEEPQSVLQ LPTSIAAGGE GLTDVSPETT TPEPPSSAAV SPGTEEPAGD TKKKIDILLK
     AVGDTPIMKT KKWAVERTRT IQGLIDFIKK FLKLVASEQL FIYVNQSFAP SPDQEVGTLY
     ECFGSDGKLV LHYCKSQAWG
//